Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com Olympus Myco Grow Bags   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1
Invisibleshr00med
Stranger
Registered: 03/10/21
Posts: 29
Saving genetics
    #27662516 - 02/17/22 11:33 AM (2 years, 2 months ago)

First, I'd like to clarify that a generation is essentially a new growth, from the spores of the growth before it, correct? And if so, there's no limit to how many generations a strain can produce?

I'm also curious as to how you can save specific mycelial genetics and know what fruits are generally going to grow from it?
For example, I fruit a tub from spore. I then clone the biggest/ fastest growing one. That clone is now on agar, and has multiple sectors growing from it. I isolate a few of the choice sectors and transfer those to new agar plates. Then, I'd A2G each of those plates and eventually fruit them, to see which of those sectors I want on a master slant. But, because I have to grow the sector out in order to see which one is the best, I'm left with nothing except the fruit bodies. So how would I be able to save a master of that culture? Spores would reset the genetics, and cloning a clone wouldn't assure me of those genetics.

Extras: Filter Print Post Top
Offlinepsilobot
Stranger
 User Gallery
Registered: 12/11/21
Posts: 18
Last seen: 19 days, 1 hour
Re: Saving genetics [Re: shr00med]
    #27662898 - 02/17/22 04:49 PM (2 years, 2 months ago)

First, some housekeeping to keep my response clear:

Tx0 = clone tissue agar plate
Tx1 = transfer from original tissue plate (Tx0)
Tx1.a = transfer from Tx0 (from sector A)
Tx1.b = transfer from Tx0 (from sector B)
.
Tx2.1a = transfer from tx1, in this case from the 1a sector plate (the .1a designation)
And so on...

Now, to answer your question, you wouldn't use the entire agar dish from any of your Tx1 plates to go A2G. Your Tx1 plates would be considered your masters, and to grow from them, you'd first transfer from them to new plates (Tx2.1a, Tx2.1b, etc.) and you'd then go agar to grain once the Tx2 plates were grown out. If you find that the fruits are not what you were looking for, shit-can the Tx1 plate and go back to your Tx0 master and try a new sector.

If the Tx2 plate yields the desired phenotype after A2G, a wedge might then be transferred from its parent Tx1 plate to a slant for long term master/storage, and the Tx1 plate could continue to be used for A2G transfers, LC, or expansion on agar.


--------------------

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: OlympusMyco.com Olympus Myco Grow Bags   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Sectoring and LC correlation- what REALLY causes sectoring: Q AmbientArmada 817 1 10/01/07 04:54 PM
by RogerRabbit
* Is this a 'non-sectoring' agar isolate? Aeolus1369 9,645 11 11/30/09 10:24 PM
by MasFina
* Transfers, Isolates, and Sectors?? Please explain
( 1 2 all )
tahoe 10,187 20 04/29/10 05:52 PM
by RogerRabbit
* another genetic question. Mindzpore 2,066 9 05/30/05 08:43 AM
by Mindzpore
* Petri dish cultures and sectors The shroomy 1 3,319 12 03/22/08 08:52 AM
by shoeareyou
* Multi-spore genetics BlueJay 1,721 4 07/20/02 02:11 AM
by BlueJay
* Sectoring or Senescence (pic) Olgualion 3,995 16 05/24/03 04:52 PM
by micro
* Mycelium & Genetic Exchange Gawain 3,241 13 10/23/02 09:42 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
539 topic views. 0 members, 3 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.015 seconds spending 0.003 seconds on 12 queries.