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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
9 year old prints =D
    #27661600 - 02/16/22 05:16 PM (2 years, 10 months ago)

Took some 9 year old prints and put them to agar about 10 days ago.. let ya know how it goes.

I have Oak Ridge 1/18/13, Thai Lipa Yai also from 2013 that I made and a Ecuador print that I also made myself in 2013.

All have been kept in small bags at room temp. Anywhere from 60 degrees to 80ish degrees.

Agar is MEA, 1000 mL water with about 24 grams of MEA. All done in front of a flow hood.


I poured new petris, took sterile cotton swabs, scooped some condensation from inside the petri to wet the swab, picked up some a fair amount of spores and streaked a z on 2-4 petris at a time.


I think that's all for now.


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OfflineForresterM
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Registered: 02/05/13
Posts: 9,352
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Re: 9 year old prints =D [Re: zMan]
    #27661606 - 02/16/22 05:20 PM (2 years, 10 months ago)

That's funny I had a thai lipa yai print from 2013 too, unfortunately I lost it.  Haven't heard that strain mentioned in a while!


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: 9 year old prints =D [Re: Forrester]
    #27661640 - 02/16/22 05:55 PM (2 years, 10 months ago)

It was probably mine then. I made a TON of them and sent a bunch out.


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OfflineForresterM
aspiring sociopath
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Registered: 02/05/13
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Re: 9 year old prints =D [Re: zMan]
    #27662089 - 02/17/22 01:40 AM (2 years, 10 months ago)

Nah I made it, it was from one of the first cube grows I ever did. Good luck hopefully yours has some viable ones!


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

Extras: Filter Print Post Top
InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: 9 year old prints =D [Re: Forrester]
    #27672237 - 02/24/22 08:08 PM (2 years, 10 months ago)

Quote:

Forrester said:
Nah I made it, it was from one of the first cube grows I ever did. Good luck hopefully yours has some viable ones!




TLY was my first grow also.


Roughly 3 weeks in, no growth, not even bacteria or mold. Give it another 2 weeks and we'll see what happened.


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Invisiblefungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,104
Re: 9 year old prints =D [Re: zMan]
    #27672253 - 02/24/22 08:21 PM (2 years, 10 months ago)

It's going to be an interesting experiment to follow. A softer agar like 1.5% or 1.7% might have better chances of germination.

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InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: 9 year old prints =D [Re: fungusul]
    #27672272 - 02/24/22 08:37 PM (2 years, 10 months ago)

I've got something similar going on with spores from an 8 year old syringe.  5 days on agar so far.  Being that they've been hydrated all this time I'm in hopes they'll germinate easier.  Keeping an eye on this thread to see how yours turn out.


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"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log

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InvisibleImatryin
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Registered: 07/06/21
Posts: 1,079
Re: 9 year old prints =D [Re: zMan]
    #27672359 - 02/24/22 09:52 PM (2 years, 10 months ago)

Good to know they are viable for so long. I'm going to start a library!

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OnlineDERRAYLD
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Re: 9 year old prints =D [Re: Imatryin] * 2
    #27672363 - 02/24/22 09:55 PM (2 years, 10 months ago)

I have germinated spores from 02 recently without too much hassle.
Didn't do anything differently other than softer agar.

Reviving an old chungus Puerto Rican and some Thai T3 as well as some original pf classic.

Edited by DERRAYLD (02/24/22 10:04 PM)

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Offlinemindfuel
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Registered: 06/07/21
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Last seen: 10 months, 23 days
Re: 9 year old prints =D [Re: DERRAYLD]
    #27672397 - 02/24/22 10:28 PM (2 years, 10 months ago)

I hope they make it. You would hope that at room temp without major fluctuations, theyll be ok.


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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: 9 year old prints =D [Re: mindfuel]
    #27685940 - 03/07/22 07:02 AM (2 years, 10 months ago)

So far mine have not done anything,  no contam,  no growth.

I'm going to try to add water to a few petri to see if it'll rehydrate them and let the rest go.


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InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: 9 year old prints =D [Re: zMan]
    #27686488 - 03/07/22 04:52 PM (2 years, 10 months ago)

Sorry to hear you've got no growth yet.  My 8 year old syringe has grown bacteria along with some white fuzz that might be mycellium.  I'll soon be trying to isolate the white fuzz.  Hopefully it's myc.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log

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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: 9 year old prints =D [Re: Elf_on_a_Log]
    #27711948 - 03/28/22 07:04 PM (2 years, 9 months ago)

Welp, I haven't done anything but watch the petris because I suck. Seems just putting the spores on plates didn't work. I will attempt the water trick and making a spore syringe sooner or later and will update the post when I do.


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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: 9 year old prints =D [Re: zMan] * 1
    #27811242 - 06/08/22 07:14 PM (2 years, 7 months ago)

Holy sh**! Today I looked over at the plates that were started beginning of February, thought I would be tossing them. 3 of them molded,  1 has some beautiful oak ridge growth and the rest are still clear!

I wasn't expecting much if anything at all but lo and behold,  growth!


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Offlinenndmt
just a speck in the cosmos


Registered: 05/18/22
Posts: 134
Last seen: 1 year, 11 months
Re: 9 year old prints =D [Re: zMan]
    #27811299 - 06/08/22 07:57 PM (2 years, 7 months ago)

Quote:

zMan said:
Holy sh**! Today I looked over at the plates that were started beginning of February, thought I would be tossing them. 3 of them molded,  1 has some beautiful oak ridge growth and the rest are still clear!

I wasn't expecting much if anything at all but lo and behold,  growth!




Wow that’s awesome. Gives me some hope, I had a print from 2012 I put on plates 3 weeks ago, still nothing. Maybe I will try soft agar as mentioned above, anyway I’ll leave these for now.

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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: 9 year old prints =D [Re: nndmt]
    #27812416 - 06/09/22 04:19 PM (2 years, 7 months ago)

It's 1 out of many plates so don't hold your breath. If you can I'd make a spore syringe and let it rehydrate for a few days then to agar, that's your beat bet.

Here's a couple pics of my anomaly.






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