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InvisibleTheConfluence
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Registered: 12/13/21
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How do spores actually work?
    #27650661 - 02/08/22 09:52 AM (2 years, 3 months ago)

So...
I purchased a handful of spore syringes and went straight into germination via agar and LC (I won't LC from spore ever again). I've had a great deal of fun playing with agar and I've very much gotten the hang of expanding my genetics in that medium.

Fast forward and I have managed to germinate a strain from a syringe, then expanded onto agar... Then again into liquid. That LC then successfully enveloped 12lbs of rye grain and is now sitting seemingly dormant in my fruiting chamber.

Shout out to Gary from fresh from the farm... Casually mentioning haploid mycelium won't fruit.

I am fairly positive the lab that I purchased my spores from takes their science very seriously... So I wonder...

How do spores even work.

Fruiting bodies produce like 4 different kinds of haploids in their spore load right? Or am I making this up?

Is the expectation that I am to first mate these spores before spawning them out?

What is the likelihood that the spores from a single mushroom will germinate out into diploid mycelium?

The tent caught a little mold anyway so they are unlikely to finish their life cycle anyway but there is a good opportunity for better understanding here.


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When two people dream the same dream, it ceases to be an illusion.


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OfflineLand TroutM
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Re: How do spores actually work? [Re: TheConfluence]
    #27650703 - 02/08/22 10:29 AM (2 years, 3 months ago)

It takes effort to catch a monokayron, where dikaryon is almost always likely.  Spores really sticks together.  In order to get a mono you need to dilute a spore solution so that single spores separate and germinate far from one another, then transfer those lone germinations to plates by themselves, and then observe that mycelium under a microscope to see if they lack clamp connections and are truly monos. 
to make it simple, chances of just getting a mono are very slim.

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InvisibleQM33
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Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: How do spores actually work? [Re: Land Trout]
    #27650715 - 02/08/22 10:34 AM (2 years, 3 months ago)

Gary also claimes to have haploids without owning a hemocytometer either, lol. Also idk if he even has a microscope....?!


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InvisibleTheConfluence
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Re: How do spores actually work? [Re: Land Trout]
    #27651387 - 02/08/22 07:29 PM (2 years, 3 months ago)

Serial dilution...
Yeah it does sound arduous.

Well that's super helpful.


--------------------
When two people dream the same dream, it ceases to be an illusion.


Philip K. Dick
Lies, Inc. (1984)


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InvisiblerhizoRider
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Registered: 12/24/13
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Re: How do spores actually work? [Re: TheConfluence]
    #27654349 - 02/10/22 08:07 PM (2 years, 3 months ago)

Maybe Alan the wizard :wizard:can chime in , and add info how the odds are.?


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InvisibleQM33
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Registered: 04/09/20
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Re: How do spores actually work? [Re: rhizoRider]
    #27654770 - 02/11/22 06:28 AM (2 years, 3 months ago)

You see ,to make a dilution, to have a ratio, you need to know your starting amount. That's what a hemocytometer does. It's a blood cell counter. It measure the volume equivalent to about 1/50-1/25 of a drop, which varies.

Gary at foulatthefarm, doesn not have one, and seems to only have, let me correct myself, a stereoscope, which is not appropriate for viewing spores, much less clamp connections.


Alan's busy I'm sure. And I'm waiting for him to get back to me ha!


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI

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OnlineThe Tao
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Registered: 09/12/19
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Re: How do spores actually work? [Re: QM33]
    #27657258 - 02/13/22 09:27 AM (2 years, 3 months ago)

Quote:

QM33 said:
You see ,to make a dilution, to have a ratio, you need to know your starting amount. That's what a hemocytometer does. It's a blood cell counter. It measure the volume equivalent to about 1/50-1/25 of a drop, which varies.

Gary at foulatthefarm, doesn not have one, and seems to only have, let me correct myself, a stereoscope, which is not appropriate for viewing spores, much less clamp connections.


Alan's busy I'm sure. And I'm waiting for him to get back to me ha!




You do a serial dilution because the neat sample is too concentrated to count accurately. Once you’ve titrated the fluid down to a concentration that can accurately be counted you multiply the cell count by the dilution factor to determine the neat call count.
That gives you your starting amount.

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InvisibleQM33
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Re: How do spores actually work? [Re: The Tao]
    #27657263 - 02/13/22 09:33 AM (2 years, 3 months ago)

And idk how the fuck you think youde do that without a compound microscope and hemocytometer. Might be able to do it with a stereoscope but you'll hate yourself probably.

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OfflineLand TroutM
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Re: How do spores actually work? [Re: QM33]
    #27657356 - 02/13/22 10:58 AM (2 years, 3 months ago)

best you can do with a stereo scope is see potential single spore germs, but you would have to transfer, from what I understand, the whole germ spore and all, grow it some and then take a part of that to a compound to look for clamp connections.  so the stereo can be very helpful for that part, but its not the final step.  You wouldn't see clamp connections/lack of with a stereoscope.  you can do it without a herocytometer, but i suppose you'd just be guessing on your dilution.

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InvisibleTheConfluence
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Re: How do spores actually work? [Re: Land Trout]
    #27663645 - 02/18/22 08:31 AM (2 years, 2 months ago)

👀
Go on...


--------------------
When two people dream the same dream, it ceases to be an illusion.


Philip K. Dick
Lies, Inc. (1984)


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OfflineLand TroutM
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Re: How do spores actually work? [Re: TheConfluence]
    #27663684 - 02/18/22 09:15 AM (2 years, 2 months ago)

I’m just regurgitating what I’ve been told, haven’t practiced it myself, so good chance I could be missing something, or misunderstanding some things.  I’m really interested in collecting monos though.  You could potentially make hybrids and confirm crosses with them, so that could be fun to do.

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