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Invisiblestagger
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Registered: 05/05/19
Posts: 1,405
Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: stagger] * 1
    #28646255 - 02/04/24 10:38 AM (3 months, 11 days ago)

I'm not meaning to come across as confrontational about this and I apologize if I did.  My first question was sincere and there's a lot I don't understand in this hobby.

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InvisibleSwabMarley
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Registered: 12/07/20
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: stagger]
    #28646262 - 02/04/24 10:46 AM (3 months, 10 days ago)

Quote:

stagger said:
I'm not meaning to come across as confrontational about this and I apologize if I did.  My first question was sincere and there's a lot I don't understand in this hobby.



Nah it’s cool bud and you may be right. Just something I’ve always been told to do and it makes sense to me so I do it. No other reason than that! If you don’t run into problems not doing that then fuckin aye! Carry on! No hostility here brother! :thumbup:

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Invisiblestagger
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: stagger]
    #28646266 - 02/04/24 10:49 AM (3 months, 10 days ago)

My coir sits in a bucket or a bin or shoebox before it all gets mixed. Sometimes it sits for weeks. My spawn gets mixed open air, most of the time without gloves on.  My liners are just garbage bags that sit in an unsealed container.  All in a dusty basement where my cats have their litter boxes.  If unclean air was such an extreme problem, is the extra layer at spawning actually stopping anything?

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Invisiblestagger
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: SwabMarley] * 1
    #28646271 - 02/04/24 10:50 AM (3 months, 10 days ago)

Quote:

SwabMarley said:
Quote:

stagger said:
I'm not meaning to come across as confrontational about this and I apologize if I did.  My first question was sincere and there's a lot I don't understand in this hobby.



Nah it’s cool bud and you may be right. Just something I’ve always been told to do and it makes sense to me so I do it. No other reason than that! If you don’t run into problems not doing that then fuckin aye! Carry on! No hostility here brother! :thumbup:



You are an awesome member and cultivator here swab!  I appreciate you brother!
:hatsoff:

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InvisibleSwabMarley
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Registered: 12/07/20
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: stagger] * 1
    #28646285 - 02/04/24 11:04 AM (3 months, 10 days ago)

Back at you bud! I agree with everything you’ve said. I have been as sloppy as hell with everything! As I said. Been hit with a bout of trich of which I’ve never ever experienced despite being literally one of the laziest cultivators going so it’s making me rethink a few things. I’m sure I’ll be back to my lazy ways soon enough when I figure this shit out.

For now though I’m trying to be ever vigilant about every aspect of my cult. I was complacent as hell β€œall these extra steps!? Pah! Why!? If what I do works!?” Yeah it works until it doesn’t! By all means though, carry on until it doesn’t! It’s exactly what I’d do!

Just as soon as I find the source of my woes I’ll be back to spawning my boxes in the living room whilst eating pizza, drinking beer and letting my cat use them as a litter tray :grin: joking, I don’t even have a cat but you get my point!

Godspeed Stagger! :thumbup:

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Offlinetree frog
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Registered: 09/14/23
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: SwabMarley] * 1
    #28646307 - 02/04/24 11:34 AM (3 months, 10 days ago)

I top dress if I'm worried about grains drying out (dead mycelium is less resilient to contamination) or shit blobbing, like APEs.

When we spawn in open air mold spores are getting mixed into the substrate.  I don't imagine top dressing is going to make a difference on that front.

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InvisibleSwabMarley
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: tree frog]
    #28646314 - 02/04/24 11:40 AM (3 months, 10 days ago)

Nah I know but like I said, it’s just a habit I picked up early on. It made sense to me so I will carry on doing it. I can’t remember who the quote belongs to but I remember reading when I first started out that you could literally take a shit in your box as you spawn and you’d still get mushrooms.

Yes we all know that spawning is not a sterile process but why tempt fate? Theres always a happy medium in my eyes. Don’t take a shit in your box but at the same time don’t do it in an ISO class 1 clean room.

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InvisibleSwabMarley
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: SwabMarley] * 2
    #28646315 - 02/04/24 11:42 AM (3 months, 10 days ago)

Anyways, we’re getting off topic so here’s some pans in some monos:

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Invisiblestagger
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: SwabMarley] * 1
    #28646593 - 02/04/24 03:51 PM (3 months, 10 days ago)

Ok yes, I understand your point while trying to narrow contam vectors.  Who was the member that would say, the only fuckin thing that matters is clean spawn!  Maybe they weren't keeping in mind straw/dung substrates and casings. 

I buckled down recently on my agar technique/practices because of how complacent (sloppy) I was becoming.  Not to mention I'm all thumbs in the sab sometimes and always bumping it with my forearms or elbows.  BRF bucks have been an awesome new tool for me but still not fool proof.  I just sent 2 jars this morning that I'll probably be throwing out 4-8 boxes in the very near future:lol:....

Anyways, same to you swab!

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Offlinealgae
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: stagger] * 2
    #28646942 - 02/04/24 10:55 PM (3 months, 10 days ago)






Spawned on the second.
Half covered half uncovered.
Feels like awesome growth. Unfortunately I lapsed on labeling. This one is the only different one from the rest. If I manage to fruit I’ll know what’s TTBVI and what’s BSR.
Fully uncovered.
Fully covered.

Edited by algae (02/04/24 10:57 PM)

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InvisibleReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,847
Trusted Cultivator
Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: algae] * 2
    #28647515 - 02/05/24 11:32 AM (3 months, 9 days ago)


First two are pan cyan bunnell, second two are pan cyan kona front and pan cambo komodo rear. 5 days old in LMP unmisted so far. 1pt spwan to 1 qt LMP sub to ~1 pt top layer for 1:3 total.


Top layer was a bit thicker this time than I usually do.


Several more jars of pans not far behind.


--------------------
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic



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Offline3.A.M
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: ReverendMyc] * 3
    #28648445 - 02/06/24 04:28 AM (3 months, 9 days ago)

2 tubs from the same 1L jar, 1/2 each, wild pan clone, 1 cased with my shitty brickies lime added

And the other uncased, both spawned into fruiting conditions, mini ez dial tubs

So far the cased is coming out on top, despite the wrong lime used.
Got a few prints of these if anyone’s interested, so far the clone loves the tubs, hopefully those genetics follow through to future gens.


--------------------


Edited by 3.A.M (02/06/24 04:31 AM)

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Offlinetree frog
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: 3.A.M]
    #28648507 - 02/06/24 06:29 AM (3 months, 9 days ago)

Do you have an idea what species of Pans?  I would like a close to wild print of Bisporus.

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InvisibleSwabMarley
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Registered: 12/07/20
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Loc: Drunken Stupor
Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: tree frog]
    #28648532 - 02/06/24 07:01 AM (3 months, 9 days ago)

Quote:

tree frog said:
Do you have an idea what species of Pans?  I would like a close to wild print of Bisporus.



+1 on that! I bet those bad boys would slap!!

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InvisibleBaba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
Loc: On Break Vacation
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: ReverendMyc]
    #28648899 - 02/06/24 12:02 PM (3 months, 8 days ago)

Where does Bisporus even occur naturally?.....ah never mind I looked it up on inaturalist and all observations listed there were made in the states.

Quote:

3.A.M said:
2 tubs from the same 1L jar, 1/2 each, wild pan clone, 1 cased with my shitty brickies lime added

And the other uncased, both spawned into fruiting conditions, mini ez dial tubs

So far the cased is coming out on top, despite the wrong lime used.
Got a few prints of these if anyone’s interested, so far the clone loves the tubs, hopefully those genetics follow through to future gens.





Nice side by side. Those are both good pin sets so far. Cased pins tend to be a bit fatter ime.

:popcorn:



Quote:

ReverendMyc said:

First two are pan cyan bunnell, second two are pan cyan kona front and pan cambo komodo rear. 5 days old in LMP unmisted so far. 1pt spwan to 1 qt LMP sub to ~1 pt top layer for 1:3 total.






Do I see knots on the cambo tray? They sure are troopers, nice.

:popcorn:

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InvisibleBaba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: Baba Yaga] * 3
    #28648910 - 02/06/24 12:10 PM (3 months, 8 days ago)

Wanted to pack up the tubs for a while but before I do I inoculated 3 jars with one of my first bisporus clones. Just could not not take them for a spin. I was naughty though and made LC straight from the BRF puck the clone tissue was taken too. No turbidity or smoke like mycelium has taken over the broth so far, should be OK I think.

gonna need more popcorn :popcorn:

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InvisibleSwabMarley
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: Baba Yaga]
    #28648956 - 02/06/24 12:33 PM (3 months, 8 days ago)

Quote:

Baba Yaga said:
Wanted to pack up the tubs for a while but before I do I inoculated 3 jars with one of my first bisporus clones. Just could not not take them for a spin. I was naughty though and made LC straight from the BRF puck the clone tissue was taken too. No turbidity or smoke like mycelium has taken over the broth so far, should be OK I think.

gonna need more popcorn :popcorn:



Excited to see what you come up with bud! If you see some light as fuck whispy myc that almost looks like cobweb… then we good to go.!! Is this the last one of the season for you baba?

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InvisibleBaba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
Loc: On Break Vacation
Trusted Cultivator
Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: SwabMarley] * 1
    #28648986 - 02/06/24 12:53 PM (3 months, 8 days ago)

Yep, will be the last one for the next 6 month or so but will be continuing doing culture work to be ready for next year and not to waste any time in the warm season. This season felt weird as I had not enough time to really get into it, all was done on the side more or less.


This is the tissue donor.


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OfflineCaptainPuffy
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: Baba Yaga]
    #28649014 - 02/06/24 01:17 PM (3 months, 8 days ago)

Have you guys noticed slow colonization when going agar to grain? The culture grew reasonably fast at .5% agar (around 1 week to fully colonize the cup), but after 2 weeks in oats it's only managed to colonize a handful of grains. It seems like it's just the ones who had physical contact with the agar and doesn't seem to want to spread.



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Offline3.A.M
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Re: πŸ„πŸ„πŸ„ PANAEOLUS IN MONO TUBS 3.0 πŸ„πŸ„πŸ„ [Re: tree frog]
    #28649024 - 02/06/24 01:24 PM (3 months, 8 days ago)

Quote:

tree frog said:
Do you have an idea what species of Pans?  I would like a close to wild print of Bisporus.



No specific idea, just pan cyan or what everyone here calls blue meanies, send a DM if you want one, I’d rather they went to people I know will grow them (:


--------------------


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