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Hindsight
Mad Scientist
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Re: Official Copelandia/Panaeolus Thread [Re: moonwatcher] 1
#27630656 - 01/24/22 08:02 AM (2 years, 2 months ago) |
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I see - Any reason you aren't using verm/peat casing? Maybe difficult to get those materials where you live?
I would not advise having a fan circulating the air. Pans don't seem to like wind much. That's why I think the JCM is such a great idea. It provides steady, yet weak airflow plus humidity plus FAE plus proper temperature.
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moonwatcher
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27630657 - 01/24/22 08:07 AM (2 years, 2 months ago) |
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Yeah I dunno why I haven't, apparently I like making my life harder than it needs to be I have verm but peat is only sold in large bags (still cheap) and I have a tiny apartment. Same with lime I have to buy 20 lbs (still for like 3 bucks) or maybe I'll use eggshells. Will probably buy some of both in the end, for sure if the manure trays suffer from the same problems. But also soil casing has always worked extremely well for cubes (I use it despite having coir and verm that's saying enough I think) so I just assumed it would be amazing for pans too.
Edited by moonwatcher (01/24/22 08:19 AM)
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: moonwatcher]
#27630713 - 01/24/22 09:00 AM (2 years, 2 months ago) |
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Quote:
moonwatcher said: Surface only about 48h after spawning (first time using poo, or composted manure to be precise). Case now or give 3-4 days of consolidation? BTW this is with grain spawn that sat in the fridge for a month, which many people are saying is a bad idea. Will see.
Any new findings on the whole consolidation thing? I can see some discussions on it a while back in this thread. A few people claimed it prevents casing colonization, others found no difference. But makes sense, if it won't fruit for 5-6 more days anyways, at the very least reduce casing contams by shortening fruiting time hmmm.
Looks surprisingly good for spawn that was in the fridge for a month!
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gt40
I will proof smthng
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27630736 - 01/24/22 09:22 AM (2 years, 2 months ago) |
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Quote:
Adas said:
Quote:
moonwatcher said: Surface only about 48h after spawning (first time using poo, or composted manure to be precise). Case now or give 3-4 days of consolidation? BTW this is with grain spawn that sat in the fridge for a month, which many people are saying is a bad idea. Will see.
Any new findings on the whole consolidation thing? I can see some discussions on it a while back in this thread. A few people claimed it prevents casing colonization, others found no difference. But makes sense, if it won't fruit for 5-6 more days anyways, at the very least reduce casing contams by shortening fruiting time hmmm.
Looks surprisingly good for spawn that was in the fridge for a month!
+
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Baba Yaga
Fomo Kills The Cat
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Re: Official Copelandia/Panaeolus Thread [Re: gt40] 3
#27630834 - 01/24/22 11:03 AM (2 years, 2 months ago) |
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uncased tub
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27630861 - 01/24/22 11:17 AM (2 years, 2 months ago) |
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Wow these Cambos will really fruit from anything!
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EnDelfisE
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27630866 - 01/24/22 11:18 AM (2 years, 2 months ago) |
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Hey friends, what do you think of this pan Jar? It doesn't seem to be very thick after I shook it ( I also think my water content was a bit on the dry side tho) and it has started forming something like blobs, should I birth it anyway?
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: EnDelfisE]
#27630875 - 01/24/22 11:25 AM (2 years, 2 months ago) |
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I would give it a shot, it's not a deal breaker when jars turn out like this after a shake and were looking fine beforehand. You won't know for sure without trying.
Look at this post from a few days ago
https://www.shroomery.org/forums/showflat.php/Number/27619866#27619866
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Baba Yaga
Fomo Kills The Cat
Registered: 09/13/20
Posts: 4,153
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27630888 - 01/24/22 11:36 AM (2 years, 2 months ago) |
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Quote:
Adas said: Wow these Cambos will really fruit from anything!
That's why I said I think my genetics are possessed by the devil. But I don't have anything else so I can't compare.
Anyone of the usual suspects interested in those genes? Shoot me a PM, need a point of reference one way or the other. No randoms please, this is for science this time and not out of good will.
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EnDelfisE
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27631132 - 01/24/22 03:22 PM (2 years, 2 months ago) |
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Quote:
Baba Yaga said: I would give it a shot, it's not a deal breaker when jars turn out like this after a shake and were looking fine beforehand. You won't know for sure without trying.
Look at this post from a few days ago
https://www.shroomery.org/forums/showflat.php/Number/27619866#27619866
Thank you!! You have given me hope with your post! It's my first time trying to grow this Gems 💙 a bit paranoid about everything haha
By the way! I figured out the food dye I used was messing up with pans ( and some cube genetics...) they were stalling sitting at a little spot not expanding at all looking all weird...
I transfered a piece on plain grain water/ brewers yeast agar and it seems to be alive again! I even see knots! May I ask which part would you transfer please?
Edited by EnDelfisE (01/24/22 03:23 PM)
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Panny
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27631147 - 01/24/22 03:39 PM (2 years, 2 months ago) |
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Quote:
Baba Yaga said:
Quote:
Grim11311 said: Hi guys. Took these pics a few minutes ago which would you say is healthy pan mycelium?
You really have to change your agar recipe, can't see anything conclusive on those plate. Just use the potato water and 1ml of syrup or honey if you can't find anything else. Or grain water like Hindsight and Adas said. If you can, whip up a few grain jars then run a few tests with those plates and see what happens, in the meantime you can sort out your agar situation.
Just out of interest, how long are these plates colonizing for to get to this stage?
what's a good agar recipe for pans?
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Panny]
#27631227 - 01/24/22 04:46 PM (2 years, 2 months ago) |
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Really any of the standard nutrients and recipes will do. LME, potato dextrose, grain water, BRF what ever you got on hand or is the easiest to acquire, just keep the overall nutrient content at around 2%.
Maybe the more sophisticated recipes are better for long term storage but for regular germination, cleanup and transfer work a simple one will do just fine.
I am using 1% LME for a while now and can't complain.
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Baba Yaga
Fomo Kills The Cat
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Posts: 4,153
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Re: Official Copelandia/Panaeolus Thread [Re: EnDelfisE]
#27631268 - 01/24/22 05:20 PM (2 years, 2 months ago) |
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Quote:
EnDelfisE said:
Quote:
Baba Yaga said: I would give it a shot, it's not a deal breaker when jars turn out like this after a shake and were looking fine beforehand. You won't know for sure without trying.
Look at this post from a few days ago
https://www.shroomery.org/forums/showflat.php/Number/27619866#27619866
Thank you!! You have given me hope with your post! It's my first time trying to grow this Gems 💙 a bit paranoid about everything haha
By the way! I figured out the food dye I used was messing up with pans ( and some cube genetics...) they were stalling sitting at a little spot not expanding at all looking all weird...
I transfered a piece on plain grain water/ brewers yeast agar and it seems to be alive again! I even see knots! May I ask which part would you transfer please?
I don't know, looks very wispy and I always have a hard time to tell what's up when the plate has fully grown out, but if I had to take a transfer then I would pick one of those little knots and transfer it to a new plate, the rest of the plate looks all the same.
EDIT:
After having a second look maybe it would be better to go with the more organized growth between 4 and 7 o'clock. The rest of the plate is not looking that great, those knots might have formed because of something else being present, probably mold.
Just my 2c.
Edited by Baba Yaga (01/24/22 06:33 PM)
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EnDelfisE
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27631791 - 01/25/22 06:20 AM (2 years, 2 months ago) |
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Quote:
Baba Yaga said:
Quote:
EnDelfisE said:
Quote:
Baba Yaga said: I would give it a shot, it's not a deal breaker when jars turn out like this after a shake and were looking fine beforehand. You won't know for sure without trying.
Look at this post from a few days ago
https://www.shroomery.org/forums/showflat.php/Number/27619866#27619866
Thank you!! You have given me hope with your post! It's my first time trying to grow this Gems 💙 a bit paranoid about everything haha
By the way! I figured out the food dye I used was messing up with pans ( and some cube genetics...) they were stalling sitting at a little spot not expanding at all looking all weird...
I transfered a piece on plain grain water/ brewers yeast agar and it seems to be alive again! I even see knots! May I ask which part would you transfer please?
I don't know, looks very wispy and I always have a hard time to tell what's up when the plate has fully grown out, but if I had to take a transfer then I would pick one of those little knots and transfer it to a new plate, the rest of the plate looks all the same.
EDIT:
After having a second look maybe it would be better to go with the more organized growth between 4 and 7 o'clock. The rest of the plate is not looking that great, those knots might have formed because of something else being present, probably mold.
Just my 2c.
Hey! Thank you again for the advise! It's true sometimes I see knots forming where contains are present? Is this a way the calture is trying to survive sensing the danger?
I transfered both the knot and nice growth just to see what's up! :p
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Wall.E
Bacteria's Bitch
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Posts: 2,860
Loc: Fungal Void
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Re: Official Copelandia/Panaeolus Thread [Re: EnDelfisE]
#27631857 - 01/25/22 08:12 AM (2 years, 2 months ago) |
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Yes, fungi will throw fruits out as a response to contamination or competition.
-------------------- Life’s shit, but I’m loving it
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Hindsight
Mad Scientist
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Re: Official Copelandia/Panaeolus Thread [Re: Wall.E] 1
#27631884 - 01/25/22 08:42 AM (2 years, 2 months ago) |
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Quote:
Wall.E said: Yes, fungi will throw fruits out as a response to contamination or competition.
Damnit, my secret is exposed. All the contamination in my trays is as a result of my deliberate actions in order to get awesome flushes
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Wall.E
Bacteria's Bitch
Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#27631886 - 01/25/22 08:47 AM (2 years, 2 months ago) |
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It’s the only reason I have mushrooms
-------------------- Life’s shit, but I’m loving it
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Hindsight
Mad Scientist
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Re: Official Copelandia/Panaeolus Thread [Re: Wall.E]
#27631897 - 01/25/22 09:00 AM (2 years, 2 months ago) |
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LOL! Well lately the only things (aside from Nats) that I've successfully grown have been trich, and also some trich. I'm digging myself out of a contamination issue that was caused by letting my 180lb dog spend too much time in my cult area, and I also had a grain weevil infestation that I think I've solved now.
On another note, I thought Instapots heated to 163F on keep warm phase but I decided to stick an instant read thermometer into the center of my bag of sub that had been on a 12 hour keep warm cycle and it read 145F. I guess that's technically still ok for pasteurization though I think it's on the lower end of the range.
Edited by Hindsight (01/25/22 09:01 AM)
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Panny
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27631959 - 01/25/22 10:11 AM (2 years, 2 months ago) |
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Quote:
Baba Yaga said: Really any of the standard nutrients and recipes will do. LME, potato dextrose, grain water, BRF what ever you got on hand or is the easiest to acquire, just keep the overall nutrient content at around 2%.
Maybe the more sophisticated recipes are better for long term storage but for regular germination, cleanup and transfer work a simple one will do just fine.
I am using 1% LME for a while now and can't complain.
I've been using a 10g LME, 10g agar powder, and 500ml water recipe. When I've tried Panaeolus bisporus and Panaeolus cinctulus on it they stalled out. I guess I'm gonna have to really dial down the LME.
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rockyfungus
dirty
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27631996 - 01/25/22 10:44 AM (2 years, 2 months ago) |
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Quote:
Hindsight said: LOL! Well lately the only things (aside from Nats) that I've successfully grown have been trich, and also some trich. I'm digging myself out of a contamination issue that was caused by letting my 180lb dog spend too much time in my cult area, and I also had a grain weevil infestation that I think I've solved now.
On another note, I thought Instapots heated to 163F on keep warm phase but I decided to stick an instant read thermometer into the center of my bag of sub that had been on a 12 hour keep warm cycle and it read 145F. I guess that's technically still ok for pasteurization though I think it's on the lower end of the range.
You know after using the Instapot for 6 failed casings. Fuck it for real pasteurization. I think it's like bucket tek. It doesn't need pasteurizing to begin with so it "works".
Just made some transfers and put wild coast to grain again. Burnells do have a bit of Aspergillus, Psilopsych. I'll make it work.
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