|
Doctor Mario
๐๐๐๐๐



Registered: 08/07/20
Posts: 1,894
Loc: ๐บ๐ธ
|
Re: Cultivation General Discussion [Re: rockyfungus] 1
#27625111 - 01/19/22 10:10 PM (2 years, 27 days ago) |
|
|
Quote:
Lemgrub said: Man I just can't figure out where this bacteria is coming from. Increased PC time & pressure, unmodded/modded lids, etc. Still get wet spots and floral or chocolate smells before shaking.

That doesn't look bacterial to me. There's a difference between condensation and sweating. Shake that jar up and let it recover. If all the grain doesn't colonize and the mycelium starts looking real thick, it's bacterial. If it looks like that again after it recovers, spawn it.
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,647
Loc: Exact Center
Last seen: 6 hours, 40 minutes
|
Re: Cultivation General Discussion [Re: Doctor Mario]
#27625166 - 01/19/22 11:05 PM (2 years, 27 days ago) |
|
|
Quote:
Lemgrub said: Man I just can't figure out where this bacteria is coming from. Increased PC time & pressure, unmodded/modded lids, etc. Still get wet spots and floral or chocolate smells before shaking.

dial the moisture back a little on your grain prep. I always had issues with millet, its always either too wet or too dry. I almost guarentee its a grain prep issue, if not then its technique.
Quote:
Doctor Mario said:
Quote:
Lemgrub said: Man I just can't figure out where this bacteria is coming from. Increased PC time & pressure, unmodded/modded lids, etc. Still get wet spots and floral or chocolate smells before shaking.

That doesn't look bacterial to me. There's a difference between condensation and sweating. Shake that jar up and let it recover. If all the grain doesn't colonize and the mycelium starts looking real thick, it's bacterial. If it looks like that again after it recovers, spawn it.
I disagree with the initial statement, but the trouble shooting i agree with.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
Lemgrub



Registered: 04/02/18
Posts: 601
|
Re: Cultivation General Discussion [Re: Doctor Mario]
#27625173 - 01/19/22 11:13 PM (2 years, 27 days ago) |
|
|
Quote:
rockyfungus said:
if spawn is clean then it's your agar to grain tek? What type of lids your running? What's your grain prep?
I just ran a test that finished up a couple days ago. I've been documenting my woes with this mysterious contamination in my journal over the last several months. The first place I looked was my SAB technique, then petri handling, then PC usage as I've moved higher in elevation and need increased PSI and more venting, and now I'm thinking of changing the grain.
I just ran modded against unmodded lids, five different sets of inoculations from different cultures. One jar developed green mold within a few days, another didn't recover after a shake. So 2 lost out of 12. Everything else colonized fine.
I boil millet 40 minutes in twice it's weight in water, let it steam off completely, load and PC
Quote:
Lemgrub said: Well the round of jars from 1/4 are finishing up. I opened the fully colonized one on the right and it smelled faintly of jasmine before shaking and like regular mush after.

There were still some wet spots on colonized grains. Don't know what to make of this. I spawned the pint to a bag, but I'm not very hopeful. If this doesn't work then the only other variable I can think to change is the grain itself.
Quote:
Doctor Mario said:
That doesn't look bacterial to me. There's a difference between condensation and sweating. Shake that jar up and let it recover. If all the grain doesn't colonize and the mycelium starts looking real thick, it's bacterial. If it looks like that again after it recovers, spawn it.
That's what's thrown me off for several months. The pint masters and quart G1's would colonize fine and most recovered after a shake at 100%. Then I noticed in G1's that there would be a few grains left uneaten and that they looked a bit off after a shake. When spawned, they'd colonize the sub with different amounts of success, some very well others poorly. The last batch of 6 shoeboxes and 2 mono's all colonized well, sat at full colonization for 3 weeks, and then all succumbed to mold. I got 27 dried grams from a 3 qt shoebox
-------------------- We getting that pirate bay, alien shishkebab cordycep money

|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,647
Loc: Exact Center
Last seen: 6 hours, 40 minutes
|
Re: Cultivation General Discussion [Re: Lemgrub] 1
#27625197 - 01/19/22 11:47 PM (2 years, 26 days ago) |
|
|
move to wheat.
its the performance king. like moving from a horse & buggy to a Ferrari
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
Lemgrub



Registered: 04/02/18
Posts: 601
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#27625203 - 01/20/22 12:01 AM (2 years, 26 days ago) |
|
|
Quote:
tryptkaloids said: move to wheat.
its the performance king. like moving from a horse & buggy to a Ferrari
I've been interested in wheat for a while, just don't know where to get it.
I suppose I'll try one of the soak and load millet teks first, then make a grain change. I still have a little RGS which worked well in the past.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

|
myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
Posts: 1,415
|
Re: Cultivation General Discussion [Re: Lemgrub]
#27625307 - 01/20/22 03:47 AM (2 years, 26 days ago) |
|
|
I've been to the mailbox approximately 1000 times looking for some incoming giveaway spores like
|
Doctor Mario
๐๐๐๐๐



Registered: 08/07/20
Posts: 1,894
Loc: ๐บ๐ธ
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#27625327 - 01/20/22 04:34 AM (2 years, 26 days ago) |
|
|
Quote:
tryptkaloids said: I disagree with the initial statement, but the trouble shooting i agree with.
I cult out of my bedroom and the room temp swings by 10-15ยฐF every 24 hours. I'm used to seeing a little bit of condensation in my jars. I always shake at 100%. My jars never smell like chocolate though. I've seen someone else compare the smell of healthy mycelium to that of a cigar. I ignore what the weird smells that people use to describe the health of their jars. Except for feet... that ones a given.
I'm also used to seeing a few pieces of grain that appear to be "uneaten" but it's always a really small portion and it's right where the grain is pressed up against the glass of the jar. Again, that's due to the condensation creating a wet spot between the price of grain and the glass. It will stop that little spot from colonizing. Again, it's only where some of the grain touches the glass and the rest of the grain is visibly colonized on the outside and I'm sure the inside as well.
I might regret saying this but I have never grown mold in a tub and haven't seen trich in a jar since getting a FFU. I've had a ton of bacterial jars but that's went down considerably since switching from oats to wheat. Wheat is definitely my favorite grain now. It's easy to prep and it steam dries really fast.
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,647
Loc: Exact Center
Last seen: 6 hours, 40 minutes
|
Re: Cultivation General Discussion [Re: Doctor Mario]
#27625370 - 01/20/22 06:13 AM (2 years, 26 days ago) |
|
|
right. but that looks a little "gooey" to be just condensation imo
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
Ababyphoenix
You



Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 10 days, 2 hours
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#27625419 - 01/20/22 07:22 AM (2 years, 26 days ago) |
|
|
All my Friends really liked APE potency but wanted something more. Any suggestions on what would be a fun next grow for potency? Im think Pan Cyans or Nats right? Something that isnt a cube.
--------------------
   Pho's MushiAventures:
My '21 GLOG LAGM 2022
|
Doctor Mario
๐๐๐๐๐



Registered: 08/07/20
Posts: 1,894
Loc: ๐บ๐ธ
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#27625425 - 01/20/22 07:25 AM (2 years, 26 days ago) |
|
|
Quote:
tryptkaloids said: right. but that looks a little "gooey" to be just condensation imo
You've been doing this a lot longer than I have and you're probably right. I normally don't chime in on posts like this because identifying less obvious contams from a two dimensional point of view is a challenge for me. I wanna be able to pick that jar up, rotate it and and inspect it from different angles. Without getting up close and personal, it's hard to know exactly what's going on with it.
I'm not saying that it isn't bacterial, I'm just saying that from my point of view the wet spots look like condensation. Any time that I've had bacterial spawn it looks similar to the picture but after a shake at 100%, it recovers like shit. Uncolonized grain and thick mycelium. Let it go long enough and either gel starts to form in the bottom or 1/3 of the jar fills up with liquid.
On a side note, it's rather strange but I've also seen less bacterial issues since I quit A2G and started ussing LC. I'm sure that it's all just an improvement in sterile technique. Yesterday, I tossed 6 bacterial jars that I nocced with LC that I made a year ago. I hadn't quite figured out how to maintain sterility in my process at that time.
|
Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
|
Re: Cultivation General Discussion [Re: Ababyphoenix] 2
#27625465 - 01/20/22 08:20 AM (2 years, 26 days ago) |
|
|
Quote:
Ababyphoenix said: All my Friends really liked APE potency but wanted something more. Any suggestions on what would be a fun next grow for potency? Im think Pan Cyans or Nats right? Something that isnt a cube.
Pans, nats, semps and tamps are all more potent than cubes, but only pans, semps and gnarly nats are as potent or more than apes
-------------------- Lifeโs shit, but Iโm loving it
|
Ababyphoenix
You



Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 10 days, 2 hours
|
Re: Cultivation General Discussion [Re: Wall.E]
#27625473 - 01/20/22 08:28 AM (2 years, 26 days ago) |
|
|
Pans it is 
Isnt there an albino pan thats like nightmare level potent?
--------------------
   Pho's MushiAventures:
My '21 GLOG LAGM 2022
|
Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
|
Re: Cultivation General Discussion [Re: Ababyphoenix]
#27625493 - 01/20/22 08:50 AM (2 years, 26 days ago) |
|
|
I think thatโs just about any pan
-------------------- Lifeโs shit, but Iโm loving it
|
SwabMarley
Twisted Metal Head


Registered: 12/07/20
Posts: 1,553
Loc: Drunken Stupor
|
Re: Cultivation General Discussion [Re: Wall.E]
#27625501 - 01/20/22 08:57 AM (2 years, 26 days ago) |
|
|
Iโve had a pan Jamaican swab for almost a year that Iโve done fuck all with yet. Might have to germinate it and give it a run. So as long as you use a decent bit of HPoo, give plenty of FAE youโre good?
|
Doctor Mario
๐๐๐๐๐



Registered: 08/07/20
Posts: 1,894
Loc: ๐บ๐ธ
|
Re: Cultivation General Discussion [Re: SwabMarley] 2
#27625502 - 01/20/22 09:00 AM (2 years, 26 days ago) |
|
|
Gonna derail a bit here but I often wonder about accidental isolation of unwanted genetics. For instance, if I took spores from a tub that took longer than normal to pin and someone else uses those spores. Will those spores also produce slow cultures or will it take a few generations for that trait to stabilize?
It's just a hunch but I think that this is likely especially when everybody and their mom is trying to make money selling spores. Good genetics get over looked when the goal is to make as many prints and syringes as possible. I'd really like to get my hands on some OG genetics or wild prints.
|
rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 11 days, 13 hours
|
Re: Cultivation General Discussion [Re: Doctor Mario]
#27625537 - 01/20/22 09:35 AM (2 years, 26 days ago) |
|
|
I'm not sure what happens here for me. Maybe hybrids? Make a huge graveyard outside of spent subs, grains, etc. Those fruits tend to be better genetics then the stuff you get inside.
Not sure if natural selection gives you much better genetics outside and inside we're really rolling the dice for "inside fruiters".
To clarify; not talking bigger fruits or more potent. They are more aggressive printed from outside and moved to agar then my indoor prints of the same "cube". I went from a cube that never pinned to a cube that usually pins on germ. Anecdotal and no clue what I'm getting at.
|
Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 1 day, 4 hours
|
Re: Cultivation General Discussion [Re: rockyfungus] 1
#27625569 - 01/20/22 09:54 AM (2 years, 26 days ago) |
|
|
I would think that given the vast amount of genetics amongst a typical colony indoors or out, that you wouldn't need to worry about inferior strains as a result of average cultivation. Life finds a way.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 14 hours, 52 minutes
|
Re: Cultivation General Discussion [Re: Failboat] 1
#27625584 - 01/20/22 10:03 AM (2 years, 26 days ago) |
|
|
Yeah itโs so hard to breed a trait into spores it would take a lot to happen randomly. But I do think weโre seeing some of that in types like GT that have been around forever. If you look at grows from 15-20 years ago theyโre so much different than now. Thatโs not to say that theyโre worse now, just much more homogenized. A lot of the old grows you see lots of giant mushrooms and lots of phenos. These days most gt grows seem to be almost clone like.
Mario Iโve got some prints from a wild Florida tub if you want. Iโve only grown them once so far so the prints are probably f3.
--------------------
LAGM2020     
Edited by A.k.a (01/20/22 10:05 AM)
|
rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 11 days, 13 hours
|
Re: Cultivation General Discussion [Re: A.k.a]
#27625606 - 01/20/22 10:25 AM (2 years, 26 days ago) |
|
|
Just look at B+. It's the inbred slutty uncle. Popping out weird double fruits and caps. My B+ was clean (Sporeworks), but if you look at the B+ some people are passing around.
Just like dogs. Went from good genes to a bunch of fucked up traits. Now some people are doing godswork and fixing all these flat nosed freaks. We're getting into the territory of dogs that can't reproduce.
We should discuss eugenics I mean inbreeding. I'd be curious for Alan to differentiate to different B+'s or GT's on a genetic level. Not sure which region that'd have to study.
|
Doctor Mario
๐๐๐๐๐



Registered: 08/07/20
Posts: 1,894
Loc: ๐บ๐ธ
|
Re: Cultivation General Discussion [Re: A.k.a]
#27625621 - 01/20/22 10:35 AM (2 years, 26 days ago) |
|
|
I appreciate all the feedback. It's an interesting topic. My thought process is that if we all agree that phenotypes become mostly stable around filal 7-10, does that concept apply to colony health, culture speed, potency and concentration of active compounds?
I'd love to take one of those prints off your hands, AKA. I appreciate the offer. incoming!
|
|