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InvisibleMushroom love
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: tryptkaloids]
    #28438194 - 08/18/23 01:01 PM (5 months, 7 days ago)

I have some single ascospore isolate of Cordyceps militaris:
If i use one isolate to colonize a substrat and after full colonisation i inocule the substrat with a ms liquid culture: only the FIRST  monokaryotic compatible culture from ms go to give his genes to my isolate? Is there the same diversity with a clone or a ms cross with a monokaryotic isolate culture ?
I hope my English is understandable, sorry for any errors (corrections welcome)


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All my work is done without SAB or LFH, just a Bunsen burner


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InvisibleMushroom love
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: trippleblack]
    #28440559 - 08/20/23 12:06 PM (5 months, 5 days ago)

Quote:

Psilotoad said:
When you use multispore each spore that germinates produces monokaryotic hyphae and each successful pairing of monokaryotic strains produces a new dikaryotic strain so when you put down multispore you have many strains of your type. That is why this mentions you want to use an isolated dikaryon. An isolated dikaryon is comprised of 2 genetically distinct types of nuclei one from each parent monokaryon. In di-mon mating only one nuclei type is donated to the monokaryon and this is not a reciprocal exchange. Each dikaryon only has two parent nuclei from their monokaryon parents or mon and di parent but with multispore you are producing many dikaryons. Once you isolate a colony or clone a fruit you have a single dikaryon this dikaryon will be comprised of 2 nuclei one from each parent monokaryon.






Quote:

trippleblack said:
Its possible that clones of mushrooms are made up of multiple mycellium colonies; not just two parents. To my knowledge this has not been directly studied in cubensis. i think it's just an assumption that a clone is only comprised of two parents.




Who is right?

So if i do a di-mon cross with a monokaryotic strain and a multispores culture, just the first fastest compatible dikaryons from the ms go to give a nuclei to my monokaryotic culture ?

will it be a means of natural selection of high-performance culture or is it only chance? (the first drop of inoculum to hit the monokaryotic strain)
I think this is the case if the substrate remains whole after colonization and there is a lot of combinations if the grain is broken before (for a multispores inoculum)


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