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Offlinetryptkaloids
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: Psilotoad]
    #28437476 - 08/17/23 06:41 PM (5 months, 8 days ago)

Quote:

Psilotoad said:
When you use multispore each spore that germinates produces monokaryotic hyphae and each successful pairing of monokaryotic strains produces a new dikaryotic strain so when you put down multispore you have many strains of your type. That is why this mentions you want to use an isolated dikaryon. An isolated dikaryon is comprised of 2 genetically distinct types of nuclei one from each parent monokaryon. In di-mon mating only one nuclei type is donated to the monokaryon and this is not a reciprocal exchange. Each dikaryon only has two parent nuclei from their monokaryon parents or mon and di parent but with multispore you are producing many dikaryons. Once you isolate a colony or clone a fruit you have a single dikaryon this dikaryon will be comprised of 2 nuclei one from each parent monokaryon.

Here this includes some more info about nuclei exchange in mon-mon and di-mon pairings

https://www.mdpi.com/2076-2607/9/6/1248/htm




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