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AyePlus
Stony Danza


Registered: 12/18/14
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Loc: Fairfield, Connecticut
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: Alan Rockefeller]
#28150293 - 01/21/23 09:45 AM (1 year, 6 days ago) |
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Bumping this back to front page but also..
Having a hard time understanding how this consistently results in 2 monokayrotic parent types when fruiting strains can consist of more than 2 parents.
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: Alan Rockefeller]
#28165510 - 01/31/23 07:20 AM (11 months, 20 days ago) |
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Quote:
Alan Rockefeller said:
Quote:
AyePlus said: Having a hard time understanding how this consistently results in 2 monokayrotic parent types when fruiting strains can consist of more than 2 parents.
What makes you think that fruiting strains can consist of more than 2 parents? Where would the extra nuclei live?
Doesnt the possibility of a a di-mon pairing or di-di pairing happening mean there can be more than 2 parent genetics?
I thought alot of basidiomycetes are capable of tetrapolar mating systems which i though meant multiple parents
I dont understand fungal breeding all that well butni was under the impression that a fruiting culture can contain multiple strains each with their own set of genetics that can be teased apart, IE sectoring and isolation. Doesnt that imply multiple sets of genetics IE more than 2?
Or Is something else happening involving genes being transferred to the parent strain through a different process.
Any reading on the topic would be helpful
I found this but its mostly about cryptococcus and jumps species to species, that gets a bit muddy for me.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025156/
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Edited by AyePlus (02/01/23 07:43 AM)
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: AyePlus]
#28182194 - 02/11/23 11:16 AM (11 months, 9 days ago) |
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Iām getting even more confused.
@Alan
Are you saying
Quote:
LtLurker said: Shrooms Dont have just 2 parents like a punnit square. They have many parent spores, some from the same fruit, so we're talking thousands of parents for each fruit.
Isnt true?
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AyePlus
Stony Danza


Registered: 12/18/14
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: Psilotoad]
#28213997 - 03/04/23 11:25 AM (10 months, 19 days ago) |
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Quote:
Psilotoad said: When you use multispore each spore that germinates produces monokaryotic hyphae and each successful pairing of monokaryotic strains produces a new dikaryotic strain so when you put down multispore you have many strains of your type. That is why this mentions you want to use an isolated dikaryon. An isolated dikaryon is comprised of 2 genetically distinct types of nuclei one from each parent monokaryon. In di-mon mating only one nuclei type is donated to the monokaryon and this is not a reciprocal exchange. Each dikaryon only has two parent nuclei from their monokaryon parents or mon and di parent but with multispore you are producing many dikaryons. Once you isolate a colony or clone a fruit you have a single dikaryon this dikaryon will be comprised of 2 nuclei one from each parent monokaryon. Here this includes some more info about nuclei exchange in mon-mon and di-mon pairings
https://www.mdpi.com/2076-2607/9/6/1248/htm
Thank you.
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