Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Mono Tub Substrate   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 19 minutes
Re: Dedikaryotization of Cubensis fruit body clones? [Re: cheor] * 2
    #27652578 - 02/09/22 04:43 PM (1 year, 11 months ago)

I have heard of a physical method to create monokaryons from a dikaryotic culture - basically put a colonized agar plate and some water into a sterile blender cup (metal ones can be autoclaved), blend it really well, and plate out the results.    Some of the colonies that grow will be monokaryons if you blend it enough to break them apart but not so much that they are completely dead.  I am not sure what the success rate is.


Extras: Unfilter Print Post Top
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 19 minutes
Re: Dedikaryotization of Cubensis fruit body clones? [Re: Psilotoad] * 12
    #28073380 - 11/28/22 04:52 PM (1 year, 1 month ago)

Julian shared his writeup on Facebook ten days ago:



Extras: Unfilter Print Post Top
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 19 minutes
Re: Dedikaryotization of Cubensis fruit body clones? [Re: cooleko] * 1
    #28075423 - 11/30/22 02:36 AM (1 year, 1 month ago)

Quote:

cooleko said:
I would have loved an intuitive explanation with recognizing the difference of nuclei in mon/dikaryons under the scope to fully complete the introduction, but google is full of those. Is this truly as simple as all monkaryons do not present with clamps with differing nuclei and by extension that all dikaryons will have a clamp between each link of two different nuclei to another?

If it is truly this simple. I may be buying a microscope in the future.




Yes that's it.  It's good to let the mycelium grow for a few weeks to make sure it didn't get a nuclei in there somewhere, and the leading edge is sometimes monokaryotic so sample from further in.

Another way to do it is to get a nuclear stain so you can see how many nuclei are present in hypha.  Safranin is one popular one, or you could use DAPI + blue light + orange filter.


Extras: Unfilter Print Post Top
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 19 minutes
Re: Dedikaryotization of Cubensis fruit body clones? [Re: AyePlus] * 1
    #28165294 - 01/31/23 12:47 AM (11 months, 20 days ago)

Quote:

AyePlus said:
Having a hard time understanding how this consistently results in 2 monokayrotic parent types when fruiting strains can consist of more than 2 parents.





What makes you think that fruiting strains can consist of more than 2 parents?  Where would the extra nuclei live?


Extras: Unfilter Print Post Top
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 19 minutes
Re: Dedikaryotization of Cubensis fruit body clones? [Re: wy35]
    #28201677 - 02/23/23 10:57 PM (10 months, 27 days ago)

Quote:

wy35 said:
What makes the peptone and glucose solution more dedikaryotizing than just water?





Maybe they are nutrients to help the mycelium heal after the physical destruction of the blender.


Extras: Unfilter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Mono Tub Substrate   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* P. mexicana strain A - fruit body - How? abert 16,784 18 04/25/11 07:54 PM
by PassiveAgressive
* Blending up a fruit body for grain innoculation Azmodeus 1,806 9 02/06/03 04:18 PM
by Azmodeus
* Moved: Multi-Spore vs. Cloning
( 1 2 all )
BigJohnson 6,553 28 04/21/03 06:10 PM
by BigJohnson
* incubation temperature for p. cubensis adroit_synth 5,672 12 01/24/04 07:27 PM
by adroit_synth
* Isolation necessary for clones grown on agar? Green_Velvet 2,080 11 09/30/02 07:50 AM
by Green_Velvet
* Cloning without agar?? Zen Peddler 2,861 7 01/30/02 08:11 PM
by SThomas
* 5 questions on cloning blackout 2,805 6 08/21/01 11:16 PM
by jonnyshaggs420
* tryptamine cubensis --->psilocin hcl extraction uneasyone 8,575 18 10/29/04 12:40 PM
by skullfarmer1979

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
3,464 topic views. 1 members, 2 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 17 queries.