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Offlinefossilshark
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Registered: 08/05/20
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Re: Dedikaryotization of Cubensis fruit body clones? [Re: cheor] * 1
    #27637772 - 01/29/22 05:24 PM (1 year, 11 months ago)

You cannot create a monokaryotic culture from a dikaryotic culture, your options are to A) fruit the clone culture so you have spores from it and then serial dilute the spore solution so you have a monokaryotic culture to breed with OR B) clamp the monokaryotic culture of the "blue meanie" to the dikaryotic culture of the clone via bullers phenomenon.

no matter how you breed its important that you have grown out the varietys you are working with enough to be able to visually identify a cross, as the only way you can confirm a cross is a cross is if the fruit carries traits from both parents.


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