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Mushroom-Hut Shop: Mono Tub Substrate, Substrate Bags

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Registered: 07/10/07
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Dedikaryotization of Cubensis fruit body clones? * 2
    #27619669 - 01/15/22 10:17 AM (2 years, 12 days ago)

I've been trying to find a way to create monokaryotic mycelium from dikaryotic mycelium obtained from  a clone of the strangest looking cube I've ever seen.

The goal being to then cross the resulting monokaryote with some particularly potent blue meanie cultures I have going....

So far I've come up with agar supplemented with  2-Deoxy-D-glucose at 5mM2

Or possibly performing mechanical dedikaryotization then growing out the fragments in a dextrose/glycine solution?


Does anyone have any input or other techniques they use or know of?

So far as I know these techniques haven't been tried on any species within this family....


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Mushroom-Hut Shop: Mono Tub Substrate, Substrate Bags


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