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OfflineSchtarker
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Registered: 12/29/21
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Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #27679651 - 03/02/22 11:58 AM (2 years, 26 days ago)

I'm on my way with this, but most of the spores I'm getting are in syringes, which I'm going to stop altogether as they're so full of contams that it's too frustrating, either directly to grain or my few attempts to agar, at least so far. This week I will try from the few prints/swabs I've been able to get, will post results for comment, would appreciate some feedback from you.

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InvisibleBaba Yaga
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Registered: 09/13/20
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Re: Oat Prep without Boiling and Drying [Re: Schtarker]
    #27679683 - 03/02/22 12:33 PM (2 years, 26 days ago)

I'm happy to comment on your agar plates but would appreciate if you post your agar pics in another thread. This is about grain prep.

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OfflineSchtarker
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Re: Oat Prep without Boiling and Drying [Re: Schtarker]
    #27679694 - 03/02/22 12:47 PM (2 years, 26 days ago)

One thing that I'm wondering - doesn't seem like anybody has the problem of oats being too dry, but I'm wondering if feed store oats still having the lemma, or outer coat on them is part of the problem for me, everything being as dry as it is. Do you find that you get good colonization on this part of the grain, same as the rest?

With rye or wheat berries, this part is removed...

My bags using rye colonize fine now that I have a laminar flow hood, although syringe contamination is as stated still a problem. That's the  main point...I'd still like to be able to use oats as in this part of the world they're still relatively cheap compared to the alternatives.

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OfflineSchtarker
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Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #27679695 - 03/02/22 12:49 PM (2 years, 26 days ago)

Yes, sorry, easy to lose track.

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InvisibleBaba Yaga
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Re: Oat Prep without Boiling and Drying [Re: Schtarker]
    #27679716 - 03/02/22 01:08 PM (2 years, 26 days ago)

Whole oats are hulled. I can't differentiate between the hull and the rest, everything gets colonized.

Getting your agar going is the way to go....and then retry some of the different oat preps.
If nothing works then try a different brand of oats.

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OfflineSchtarker
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Last seen: 2 years, 25 days
Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #27679806 - 03/02/22 02:58 PM (2 years, 26 days ago)

Sorry to ask you this, but there might be some confusion about the "hull" and the "lemma", which is the technical name for the papery split covering over the seed that's actually quite tough. I might have screwed up and purchased the wrong kind of oats. Horses obviously don't mind it, but they might not be the right thing for spawn... if you could post a photo of what you use that would help me.

If I have to toss the two bags I have left, or give them to somebody with a hungry burro, so be it...

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InvisibleBaba Yaga
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Re: Oat Prep without Boiling and Drying [Re: Schtarker]
    #27679828 - 03/02/22 03:20 PM (2 years, 26 days ago)

Feed oats for horses are fine. There are photos of what I use in the first post.

Go agar and then retry your grain once you are able to produce clean cultures if it doesn't work out then try another source of oats. If
you can't make oats work then try some other grain. This is all I can tell you.

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Offlinegt40
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Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #27706473 - 03/24/22 04:43 AM (2 years, 5 days ago)

hey, without a rinse, right?
I notice really dark water after soaking which is scary each time and I'm start rinsing automaticly)

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InvisibleBaba Yaga
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Re: Oat Prep without Boiling and Drying [Re: gt40]
    #27706955 - 03/24/22 01:15 PM (2 years, 4 days ago)

I give em a brief rinse when they are in the colander and then let them drip dry.
Not sure if it is necessary, it's more of a habit.

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InvisibleFloret
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Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #28144496 - 01/17/23 02:09 PM (1 year, 2 months ago)

Josex did an experiment with various preps, one being a cold soak as a "bad prep". Ultimately, it seemed like they had bad quality oats. But one of the hypotheses that came from it is that a cold soak is basically fermenting the oats and allowing bacteria to grow, which excrete substances to inhibit growth of other organisms. Those excretions may not break down at the temperatures we typically sterilize grains.
https://www.shroomery.org/forums/showflat.php/Number/27382275/fpart/1/vc/1

Have you noticed a cold soak limiting mycelium growth?

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InvisibleBaba Yaga
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Re: Oat Prep without Boiling and Drying [Re: Floret]
    #28144534 - 01/17/23 02:39 PM (1 year, 2 months ago)

Haven't experienced any problems that I could relate back to the cold soak. I have however experienced that some grains I bought were more problematic than others and that is regardless of the method of preparation.

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InvisibleFloret
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Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #28145398 - 01/18/23 03:27 AM (1 year, 2 months ago)

Thanks, too bad there's no way to determine if oats are poor quality until you try to spawn them :frown: how'd you narrow it down to a specific bag of oats?


--------------------
LAGM 2024

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InvisibleBaba Yaga
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Re: Oat Prep without Boiling and Drying [Re: Floret] * 1
    #28145458 - 01/18/23 06:09 AM (1 year, 2 months ago)

Troubleshooting can be a real pain in this hobby, it gets a little bit easier though after having grown for a while and having confidence in your routine. When problems start after switching to a new grain then you will look at that possibility first. It gets more complicated if you change more than one thing at the same time.

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OfflineGlazedHazels
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Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #28400178 - 07/18/23 11:10 PM (8 months, 5 days ago)

I am intrigued, and have a question for you.

How do you prefer to pulverize coir?

Will throwing half a brick of solid coir brick without pulverizing it into the soak and then churning up the oats and coir with a stick after an hour  be adequate?

I have really dry and powdered horse manure, will that work in place of the coir?

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InvisibleBaba Yaga
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Re: Oat Prep without Boiling and Drying [Re: GlazedHazels]
    #28400201 - 07/19/23 12:06 AM (8 months, 5 days ago)

I add the verm to suck up the wetness that is clinging to the kernels so it is only useful to add coir or verm once you have drained the grain, won't do anything if you add it to the soaking water. Having said this, I don't even add verm to every batch as I said, the grain will suck up the excess by itself and with the new batch of oats I bought after I wrote this it doesn't even make a difference. So do a side by side with and without adding "the adsorbent" and see how it goes. Don't know about dry manure, you will have to check it out yourself.

I haven't done this with coir much as verm is easier to work with but whenever I need pulverized coir then I do break it down roughly first, then I put it through a vintage ice crusher and finally rub it between my hands to get it really fine......too much work really

This is the ice crusher I used, also used it do break down dry manure. Not using it much anymore since I stopped working with dry manure.






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InvisibleFloret
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Registered: 01/02/23
Posts: 275
Loc: underneath the water
Re: Oat Prep without Boiling and Drying [Re: Baba Yaga]
    #28621646 - 01/14/24 11:52 PM (2 months, 11 days ago)

Sorry to necro this. I found this in the gourmet forum, soak only prep for other grains.

https://www.shroomery.org/forums/showflat.php/Number/22524362#22524362

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