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Offlinestromam
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Psilocybe cyanescens MS on agar, contaminated or good?
    #27615947 - 01/12/22 08:28 AM (3 years, 5 days ago)

I got a MS syringe and attempted to grow it out on agar by flaming a scalpel then cooling it in the receiving dish and then dropping a drop of the well mixed syringe onto the cooled scalpel, then slicing the scalpel through the agar several times in each sector. 

When I poured this batch of plates I had a little agar left over so I poured it into a clean, but non-sterile plate, outside my still air box, just to see what kind of contaminates I might have floating around in my room.  Why not.

Here is a picture of my MS plate and purposely contaminated plate:



I can't tell if my growth is mycelium or contamination.  Of course the contaminated plate could be contaminated with mushroom spores from other projects, but neither are showing the bleaching effect I've seen from other mycelial growth, one of mine pictured below.



Thanks again for the advice.

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OfflineLand TroutM
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: stromam]
    #27615958 - 01/12/22 08:40 AM (3 years, 5 days ago)

I’d have to say that’s mold.  I’m Unsure of what you mean by the last plate, are they another project, not cyanescens?  Two of the sectors in that last plate don’t look so good.

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Offlinestromam
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: Land Trout]
    #27616568 - 01/12/22 07:08 PM (3 years, 5 days ago)

The last plate was just showing what I though the mycelium should do when it bleached the purple dye turning it blue.  That plate was an oyster mushroom.  I flipped over my P. cyan plate and it actually did bleach the purple dye completely, it was just so thick I couldn't see through it.



I thought the two oyster sectors looked a little funny, growing in just one direction, but I didn't see any contamination, so I didn't worry too much about it.  What do you think might be wrong with them?

For the cyans, is there any way to verify if it's mold or mycelium?  I thought I read somewhere that the common fluffy molds are grey, not white and that white typically means mycelium.

Here are a couple more pictures.




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InvisibleBaba Yaga
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: stromam]
    #27616583 - 01/12/22 07:17 PM (3 years, 5 days ago)

I see mold as well, the top left section on the oyster plate (last photo in first post) looks alright.

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Offlinestromam
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: Baba Yaga]
    #27616600 - 01/12/22 07:28 PM (3 years, 5 days ago)

Well bummer.

Any idea what might be wrong with the other two sections of the oyster plate?

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OfflineLand TroutM
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: stromam]
    #27616621 - 01/12/22 07:50 PM (3 years, 5 days ago)

Some molds are bright white.  I’m going off the date on the plate.  I’ve seen several different cultures of cyanescens on plates and I’ve seen growth that looks like that, but not that aggressive,

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InvisibleBaba Yaga
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: Land Trout]
    #27616674 - 01/12/22 08:46 PM (3 years, 5 days ago)

The other two sections seem to have bacterial contamination. That is why the oyster mycelium doesn't grow out properly all the way around.
Top right between 11-1 o'clock and bottom 6 o'clock locks alright.

Still plenty to transfer from the top left section so no worries.

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InvisibleAshtray161
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: Baba Yaga]
    #27616815 - 01/12/22 11:42 PM (3 years, 4 days ago)

Contamed/mold IMO, sorry for your loss


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Offlinestromam
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: Baba Yaga]
    #27623894 - 01/18/22 06:39 PM (2 years, 11 months ago)

I know I'm hijacking my own thread a bit, but I transferred that section of oyster to a new plate and voila.



I'm happy with this one.

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Offlinevem
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Registered: 05/28/22
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: stromam]
    #27796963 - 05/28/22 09:44 PM (2 years, 7 months ago)

Dont mean to hijack, but I got bitty little white worms in 9 petri dishes.
How do I start a thread. What can I do

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Offlinevem
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Re: Psilocybe cyanescens MS on agar, contaminated or good? [Re: vem]
    #27796965 - 05/28/22 09:44 PM (2 years, 7 months ago)

how do a new thread for white worm agar?

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