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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 16 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u]
#28263199 - 04/05/23 08:26 AM (9 months, 19 days ago) |
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Quote:
Shroomin4u said:
Which is why no one on this forum will ever get any advice from me again.
Oh no, what a loss to the community.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Shroomin4u
Shroomer

Registered: 02/18/22
Posts: 43
Last seen: 9 months, 18 days
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: dyel]
#28263207 - 04/05/23 08:30 AM (9 months, 19 days ago) |
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Quote:
dyel said: i assume laminar flow is a range and not a number or else it will be only a line and not an area (im referring to the working area in front of the hood) since flow will lose velocity due to friction and physics stuff wikipedia is saying something about Reynolds number but I'm still not completely clear about it guess I'll need to do some research then
The way my brother (who's a chemical engineer) explained laminar flow to me is a uniform flow of a fluid or liquid through a pipe (laminar flow is calculated by the dimensions of that pipe) the HEPA scheme just co opts that and so the actual equation for determining laminar flow needs to be modified to fit the paradigm of forcing air through a filter and not a pipe.
Laminar flow with a filter is determined by the work of the fan (how much cubic air it can displace in a set amount of time) and the resistance and dimensions of your housing+filter. If you do a little research on the filter you buy they'll have some literature usually for the parameters you need to meet for stable sterile air flow.
(If you want to fully understand laminar flow you'll need to work on a chemical engineering degree. Because that's the profession it's basically built for. They use it to calculate the gases and chemicals they need to flow through the various reactors and systems. It's important for them to know it to prevent back flow that would blow up their designs. Also to optimize the reactant and catalysts mixing or whatever.)
-------------------- Those who act like they know all, know nothing.
Edited by Shroomin4u (04/05/23 08:34 AM)
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: dyel]
#28263215 - 04/05/23 08:36 AM (9 months, 19 days ago) |
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Quote:
dyel said: i assume laminar flow is a range and not a number or else it will be only a line and not an area (im referring to the working area in front of the hood) since flow will lose velocity due to friction and physics stuff wikipedia is saying something about Reynolds number but I'm still not completely clear about it guess I'll need to do some research then
From my understanding the 90 for per second number was developed because it would carry particulate across the average size room and wouldnβt feel like a strong breeze blowing at the face of the cultivator but I believe that anywhere up to 350 fps can still be consideredβs Laminar although the chances of causing turbulence through poor technique are a little higher so I would try to stay in the 150-ish range
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 16 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u] 1
#28263217 - 04/05/23 08:37 AM (9 months, 19 days ago) |
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Fluids AND liquids? Wow, no way.
Now get him to explain to you that all liquids are fluids.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u] 2
#28263231 - 04/05/23 08:46 AM (9 months, 18 days ago) |
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Listen, ma'am. This is a forum dedicated to the cultivation of mushrooms. Fungal culturing falls under the umbrella of mycology and microbiology. Everything found within the text of the OP has been adapted from the science-based operating procedures of those disciplines.
Everything found in the OP is supported by objective science, not subjective opinion. Everything described is foundational, understood, accepted/received knowledge employed by both disciplines. If you have an issue with anything listed or stated in the OP then the issue is lays with you.
You can caterwaul all you like but you will find it difficult to kick against the pricks of received scientific wisdom; Furthermore: this is a drug forum full of smartasses, if you can't take it, I get it, but the next time you derail my thread with wook bullshit I'll slap you with a 30 day time out so that you can focus more on devil stick drills, spoon playing, and poncho knitting. Prrhaps then you'll learn some manners/communication skills.
Capeesh?
Also:
Quote:
Shroomin4u said:
laminar flow to me is a uniform flow of a fluid or liquid through a pipe (laminar flow is calculated by the dimensions of that pipe) the HEPA scheme just co opts that and so the actual equation for determining laminar flow needs to be modified to fit the paradigm of forcing air through a filter and not a pipe
Laminar flow with a filter is determined by the work of the fan (how much cubic air it can displace in a set amount of time) and the resistance and dimensions of your housing+filter. If you do a little research on the filter you buy they'll have some literature usually for the parameters you need to meet for stable sterile air flow.
Sounds familiar, did you even read the OP? 
Quote:
Shroomin4u said: I no longer care about anyone or anything on this site, in fact I'm going to mog on all of you and never speak a single word of my results. (Already have.)
I'm not surprised the mods muted me for not circle jerking like their butt buddies. Some poor woman came here asking if cat food would work in agar and she gets unsolicited life advice about how shit she is as a wife and mother...
Which is why no one on this forum will ever get any advice from me again. I'm not going to feed them information when all they've ever done is offer whack advice about SABs or teks.
The only reason I'm still here is because I'm keeping a eye out for anyone who's interested in mycology and isn't either a total loser (read morally bankrupt) or corrupted by power.
Stay on topic
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Shroomin4u
Shroomer

Registered: 02/18/22
Posts: 43
Last seen: 9 months, 18 days
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap]
#28263271 - 04/05/23 09:13 AM (9 months, 18 days ago) |
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Quote:
SirPsycho said:
Quote:
Shroomin4u said:
Which is why no one on this forum will ever get any advice from me again.
Oh no, what a loss to the community.

Quote:
Stipe-n Cap said: Listen, ma'am. This is a forum dedicated to the cultivation of mushrooms. Fungal culturing falls under the umbrella of mycology and microbiology. Everything found within the text of the OP had been adapted from the science-based operating procedures of those disciplines.
Everything found in the OP is supported by objective science, not subjective opinion. Everything described is foundational, understood, accepted/received knowledge and equipment employed by both disciplines. If you have an issue with anything listed or stated in the OP then the issue is lays with you.
You can caterwaul all you like but you will find it difficult to kick against the pricks of received scientific wisdom; Furthermore: this is a drug forum full of smartasses, if you can't take it, I get it, but the next time you derail my thread with wook bullshit I'll slap you with a 30 day time out so that you can focus more on devil stick drills, spoon playing, and poncho knitting. Prrhaps then you'll learn some manners/communication skills.
Capeesh?
Also:
Quote:
Quote:
Shroomin4u said:
Laminar flow with a filter is determined by the work of the fan (how much cubic air it can displace in a set amount of time) and the resistance and dimensions of your housing+filter. If you do a little research on the filter you buy they'll have some literature usually for the parameters you need to meet for stable sterile air flow.
Sounds familiar, did you even read the OP? 
Stay on topic 
bruh stop spamming me. I don't care if you guys spaz out from basic science just dont @ me. I would block and ignore you all instead of dropping a comment but that apparently doesn't work.
-------------------- Those who act like they know all, know nothing.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 16 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u]
#28263274 - 04/05/23 09:14 AM (9 months, 18 days ago) |
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Wait? You can't ignore moderators? No way
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Shroomin4u
Shroomer

Registered: 02/18/22
Posts: 43
Last seen: 9 months, 18 days
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap]
#28263282 - 04/05/23 09:18 AM (9 months, 18 days ago) |
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Wow I thought you were some rando, but when I went to block and ignore you it says your a admin. How shameful, it's like you're a 15 year old on discord. No wonder you're husband site muted me.
-------------------- Those who act like they know all, know nothing.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u] 3
#28263288 - 04/05/23 09:20 AM (9 months, 18 days ago) |
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Hope you enjoy your 30 days in the corner. Stay on topic next time. This is strike 2 for the same thread and infraction.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 16 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap] 2
#28263300 - 04/05/23 09:27 AM (9 months, 18 days ago) |
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--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
π
π
£π
£π
π
π
π
π
π
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: SirPsycho] 5
#28263312 - 04/05/23 09:35 AM (9 months, 18 days ago) |
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One does not simply fook aboot without finding oot
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myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
Posts: 1,415
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: mushboy] 3
#28263357 - 04/05/23 10:04 AM (9 months, 18 days ago) |
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Quote:
mushboy said: Canβt this thread go one page without someone getting a ban? .
I think we went 2 pages.
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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!π·
Last seen: 12 hours, 58 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u] 1
#28263364 - 04/05/23 10:09 AM (9 months, 18 days ago) |
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Quote:
Shroomin4u said:
Quote:
San Pedro Girl said:
Quote:
Shroomin4u said: Two points, stop calling me a noob if you don't like ad hominem you hypocritical oaf. Although I suspect this was bait to get me to attack you.
Second point. You are purposefully ignoring the price of HEPA filters and the fans that can overcome those kind of resistances. They range from a minimum of 200-900$
Gaskets, hinges, latches, etc. Plywood, 2x4s, etc. Cost a extremely small fraction of that. Not to mention you have to match your fan and filters with laminar math (because laminar flow is a mathematic equation.) A good quality GB would cost a fraction of what a laminar flow hood would.
As well SABs are just primitive Glove boxes. Or rather glove boxes are just upgrades to still air boxes.
Pretending like I want to slap gloves into drilled holes in a plastic bin is just what you want to hear because you can't even imagine that you're wrong. So you'll just contuine living in delusion and beleiving that I'm not as smart as you (how sad.)
-sidenote You don't need inert gas for a glovebox, but you could easily purchase said gas from a welding shop if you wanted to use it.
The crew around here are more about practical application vs speculation. A lot of members grow well using SABs and FFUs. Hell, some even do open air transfers. A lot have failed or discovered the useless/inferior nature of glove boxes, but the point is they are speaking from experience. The best way to prove your point is to knock out some amazing, contamination free grows with your glove box, showing that with the same money/time commitment you get better results than other methods.
I promise that Stipe-n cap, et al will shut up and respect you for demonstrating innovation with results. Or if you come back after trying and failing, but learn from that failure, theyβll also respect you. I think what theyβre having a hard time with is obstinance sans results.
Welcome and good luck!π
I no longer care about anyone or anything on this site, in fact I'm going to mog on all of you and never speak a single word of my results. (Already have.)
I'm not surprised the mods muted me for not circle jerking like their butt buddies. Some poor woman came here asking if cat food would work in agar and she gets unsolicited life advice about how shit she is as a wife and mother...
Which is why no one on this forum will ever get any advice from me again. I'm not going to feed them information when all they've ever done is offer whack advice about SABs or teks.
The only reason I'm still here is because I'm keeping a eye out for anyone who's interested in mycology and isn't either a total loser (read morally bankrupt) or corrupted by power.
I was quoted here, but canβt tell if this is a reply to me. If so, I was just trying to provide the best advice I could and didnβt mean any offense, so sorry if it come off that way.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: San Pedro Girl] 6
#28263378 - 04/05/23 10:23 AM (9 months, 18 days ago) |
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Let's not allow that individual to further derail the thread. They're on 30 day shore leave to ponchovilla.
So, how about that laminar flow, pretty epic amirite? I mean, still air is pretty dope, but that lamina? So orderly, so parallel....it's almost like air has become transmuted into a fluid.
A fluid shaped by pressure, geometry, and velocity to flow smoothly without lateral mixing π
Sure does beat that old still air, or does it? I guess there are pros and cons associated with each design, pretty hard to take decent spore prints in flow, for example; or how about those moldy wild samples taken while foraging? Wow, science sure is interesting.
I'm glad we don't require a degree in engineering to grasp these simple principles. With just a little bit of consideration I bet even a child could grasp the fundamental physical principles which govern still air, turbulent flow, and laminar flow.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 16 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap]
#28263383 - 04/05/23 10:27 AM (9 months, 18 days ago) |
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Since you allude to this in your comment. What's your thoughts on working with contam in front of a LFH?
Does it really just spread the bad stuff all over your lab or w/e as some people suggest?
Is it really any worse than all the baddies already floating around?
Should it be done in an SAB instead?
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
π
π
£π
£π
π
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bobthehardore
Mr Dummy


Registered: 05/13/22
Posts: 114
Loc: ComeFind me in VA
Last seen: 1 month, 10 days
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: SirPsycho] 1
#28263400 - 04/05/23 10:36 AM (9 months, 18 days ago) |
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This is my mod lid "unbodified" SAB tub for this year with many successful grows. First saw this unmodified tub used by 90sec Mycology on YT. Used it like he does last year with success. Anyways....
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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!π·
Last seen: 12 hours, 58 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: bobthehardore] 1
#28263403 - 04/05/23 10:38 AM (9 months, 18 days ago) |
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Quote:
bobthehardore said: This is my mod lid "unbodified" SAB tub for this year with many successful grows. First saw this unmodified tub used by 90sec Mycology on YT. Used it like he does last year with success. Anyways....

Looks simple and effective, but even the picture makes my back hurt.
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Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: San Pedro Girl] 2
#28263406 - 04/05/23 10:41 AM (9 months, 18 days ago) |
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Quote:
San Pedro Girl said:
Quote:
bobthehardore said: This is my mod lid "unbodified" SAB tub for this year with many successful grows. First saw this unmodified tub used by 90sec Mycology on YT. Used it like he does last year with success. Anyways....

Looks simple and effective, but even the picture makes my back hurt.
Exactly what I was thinking. That's a young person's SAB if I ever saw one.
By the way, I have my SAB on an adjustable standing desk and it is a godsend.
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: SirPsycho] 3
#28263410 - 04/05/23 10:43 AM (9 months, 18 days ago) |
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Quote:
SirPsycho said: Since you allude to this in your comment. What's your thoughts on working with contam in front of a LFH?
Does it really just spread the bad stuff all over your lab or w/e as some people suggest?
Is it really any worse than all the baddies already floating around?
Should it be done in an SAB instead?
I wouldn't be worried about spores contaminating the lab space, especially not while running the hood which will inevitably scrub the air clean of spores. The issue would be inhaling spores or live mold mycelium, some species are pretty harmful/dangerous.
Turning off the hood for a moment while working on moldy plates can work but your best bet is still air. Most moldy plates can be discarded, however you may have a single foraged specimen that cannot be tossed.
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: SirPsycho] 3
#28263418 - 04/05/23 10:49 AM (9 months, 18 days ago) |
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Iβm not stipe and i want to hear his comment too but I think it depends , how clean is your lab? If its a really nice clean positive pressure room you probably shouldnt fuck around and find out, if you have a hood set up in your moldy garage then fuckit. But when my lab was half assed (two hoods set up in a sealed room) Iβd shut them off and do the transfer. Now that my lab is alot nicer and If I really really want the culture and the contam is super bad (read:sporulating heavily) Iβve had good luck do it old school βopen airβ under an alc lamp on my dining room table, I barely open the lid and do it as quickly as possible closing lids in between cut and transfer, I do as many as possible, and as soon as i see growth from target organism i get it back in front of the hood and transfer away and go back to normal clean up procedures. Mold Spores generally take a couple days to germinate and a couple more to sporulate and usually the myc outgrows it enough to get a transferβ¦
-------------------- Learn about breeding
  C10βs agar guide Good surface conditions = Good pinsets Read more, post less. π
π° πΌ π΄ π
π΄ π° πΌ π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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