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OfflineCocaineBuffet
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: San Pedro Girl] * 5
    #28240474 - 03/21/23 09:08 PM (10 months, 2 days ago)

I have it sitting on a few of the packaging strips it came with. Reduces the vibration of the table it is on. Not elegant but it works :shrug:


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: San Pedro Girl] * 2
    #28240539 - 03/21/23 09:34 PM (10 months, 2 days ago)

I used the same material that came with the hood to also brace it against the wall so it doesn't tear it up. I took one piece and folded it in half so its roughly about 3" between the wall and the hood. Another what should be a temporary solution that has been going on long term.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: thebraintickler] * 4
    #28362537 - 06/16/23 08:15 PM (7 months, 8 days ago)

Quote:

thebraintickler said:
You can just go oldschool and light a bunch of candles around your workspace, or use burners if you have gas.  This will heat and cause the airflow to go up and out of your workspace.  It keeps the contamination down significantly, but not as good as a laminar flow hood might. 

With laminar flow hoods you are looking for two things, you want a negative vacuum pulling on the air, and you want the airflow to be sterilized.  This means you are generally using nanpore filters and a fan system blowing into the hood, with the airspace that allows the air to escape being smaller so that the airflow pushes potential airborne contamination out of the hood. 

If you are really picky about potential contam and want to get all cell biologist on it use a 90% ethanol spray around the edges before and after you move anything in and out.  It's excessive for most of this kind of fungal growth, though transferring to a liquid culture with something like PDB/TSB and then incubating at any elevated temperatures is going to require significant sterile technique, I find more so than when plating, as you can't discern the contam in liquid cultures necessarily. 

Globe boxes are intended to be kept completely sterile with positive pressure flow.  The gloves are attached to the box and anything in/out of the box goes through an exchange box where the sample container is further sterilized, UV is used a lot, but so would EtOH/IOH in combination.  Once inside everything is considered sterile.  This is overkill for things that aren't ebola.




Yeah light candles and spray flammable chemicals while using a glove box. Totally not a recipe for disaster.

Don't listen to this bullshit.


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OfflineCocaineBuffet
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap]
    #28470505 - 09/15/23 09:30 PM (4 months, 10 days ago)

Dude that is actually cool.

He is also very optimistic that the world will be habitable in the next 10K years. I give it 3 or 400 years at MAX. If we don't kill each other to oblivion the sun is going to burn out before that.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: SupaThaRipper]
    #28513465 - 10/21/23 08:36 PM (3 months, 5 days ago)

Not the correct thread, but as Stipe said they are an easy unit to make.

I got a Thermohelix unit and haven't had any issue with it. I like supporting a small business. Thermohelix is very quick to respond to questions or issues on IG so that has been convenient.

Having said that; you can get the same job done with a torch for less money and it takes up less space.


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