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OfflinePandaskis
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: thebraintickler] * 4
    #28362748 - 06/16/23 10:45 PM (7 months, 8 days ago)

Quote:

thebraintickler said:
You can just go oldschool and light a bunch of candles around your workspace, or use burners if you have gas.  This will heat and cause the airflow to go up and out of your workspace.  It keeps the contamination down significantly, but not as good as a laminar flow hood might. 

With laminar flow hoods you are looking for two things, you want a negative vacuum pulling on the air, and you want the airflow to be sterilized.  This means you are generally using nanpore filters and a fan system blowing into the hood, with the airspace that allows the air to escape being smaller so that the airflow pushes potential airborne contamination out of the hood. 

If you are really picky about potential contam and want to get all cell biologist on it use a 90% ethanol spray around the edges before and after you move anything in and out.  It's excessive for most of this kind of fungal growth, though transferring to a liquid culture with something like PDB/TSB and then incubating at any elevated temperatures is going to require significant sterile technique, I find more so than when plating, as you can't discern the contam in liquid cultures necessarily. 

Globe boxes are intended to be kept completely sterile with positive pressure flow.  The gloves are attached to the box and anything in/out of the box goes through an exchange box where the sample container is further sterilized, UV is used a lot, but so would EtOH/IOH in combination.  Once inside everything is considered sterile.  This is overkill for things that aren't ebola.




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OfflinePandaskis
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: xVadisx]
    #28433522 - 08/14/23 09:46 AM (5 months, 11 days ago)

My guess is that it seals things


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OfflinePandaskis
Eating Bamboo
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Registered: 03/14/23
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Last seen: 1 month, 1 day
Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Mycolorado]
    #28443458 - 08/23/23 05:33 AM (5 months, 3 days ago)

Ever seen a full canopy on a cow pat?


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