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OfflineSirPsycho
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: sandman420] * 2
    #28120025 - 01/01/23 01:00 PM (1 year, 26 days ago)

Quote:

sandman420 said:
HOW TO REALLY BUILD A FLOW HOOD, REAL METH LOVERS ONLY





FTFY


--------------------
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Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Drmbanana] * 2
    #28126375 - 01/05/23 06:45 PM (1 year, 22 days ago)

Quote:

Drmbanana said:
Thanks for you insight, just trying to get a low tech method, and I am cheap as hell, so…… if something that is not a SAB gets me around 10% contam, I’m good. I will look into smuvbox.



An SAB can get you 0% contam rate :shrug:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: PsillySeeEms] * 2
    #28126890 - 01/06/23 06:05 AM (1 year, 22 days ago)

Quote:

PsillySeeEms said:

Or, you guys could just shell out the +/- $100 too, for a pack of clear trash bags, a holmes desktop unit, and a purple filter. See how it works for yourselves. Haha





Or I could spend a few bucks on a plastic box and know it works.

But if you show me a smoke test with your laminar bag I'll eat my shoe and suck your dick.

There's no "almost" laminar btw. It's a binary type thing. It's either laminar or it isn't. Like sterility, it's 0 or 1. True or false.

Also 10% contam is beyond horrible. Less than 1% is acceptable, at 3% you need to start asking questions, at 5% you need to re-evaluate your whole process


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: schpat] * 1
    #28129389 - 01/07/23 02:19 PM (1 year, 20 days ago)

Quote:

schpat said:
It's the hot line that rings whenever someone posts about how great a zip up PVC SAB is  :rofl:



"Look, up in the sky. It's the Cap Signal. Quickly to the Stipe mobile"


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: Stipe-n Cap] * 6
    #28131962 - 01/09/23 09:57 AM (1 year, 18 days ago)

What if I attach a shower curtain to this? I think it'll be laminar cause the plastic will make all the air go in one direction



:specialralph:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Registered: 01/01/20
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: PsillySeeEms] * 2
    #28132003 - 01/09/23 10:24 AM (1 year, 18 days ago)

Quote:

PsillySeeEms said:when know what I THINK I know



FTFY


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Registered: 01/01/20
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: Drmbanana]
    #28142775 - 01/16/23 12:14 PM (1 year, 11 days ago)

Quote:

Drmbanana said:


Just a quick incense test. There is definitely turbulent air, but I can’t imagine that is worse than just open air. Also less confining than SAB. Is it laminar? Nope. Is clean air blowing in the direction of work? Looks like it to me.

My numbers are 2 contam agar out of 100+ so far.

I would have made a new thread, just can’t seem to figure out how, sorry to hijac.



I can imagine it's worse than open air. That shit is blowing all over the place, not gonna be quite as drafty in open air.

And if you use techniques such as bunsen burner "sterile field", open air drafts become even more predictable.

BTW, my open air transfer success rate is 100%


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Registered: 01/01/20
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: Drmbanana]
    #28142836 - 01/16/23 12:56 PM (1 year, 11 days ago)

Quote:

Drmbanana said:
:eek:

Wait, what?! Open air transfers?! Mind blown….

:bigbang:



:doublejointed:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Registered: 01/01/20
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: PsillySeeEms] * 1
    #28163220 - 01/29/23 04:57 PM (11 months, 22 days ago)

Quote:

PsillySeeEms said:
My shmow hood does have a magical ionizer built in. :wink:


I do wonder though, if some people have trouble with static electricity.. not just working with a flow hood, but even in a SAB too. Imagine, you walk across the carpet to your SAB work area, dragging your feet the whole way, without even thinking about it. Then you grab a container to put inside, and it becomes charged too, sucking particles directly towards it. Something like that anyway. I guess I'll have to study all about it now, as I'm very curious about it all.



Oh shit you built it, does that mean we finally get that demonstration thread you promised a month ago or are you just gonna go on with your lecture?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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I'm a teapot User Gallery


Registered: 01/01/20
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: PsillySeeEms] * 3
    #28163433 - 01/29/23 07:37 PM (11 months, 22 days ago)

Yeah, that's the one. Lovely drawing btw.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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I'm a teapot User Gallery


Registered: 01/01/20
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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: PsillySeeEms] * 2
    #28163480 - 01/29/23 08:15 PM (11 months, 22 days ago)

Bet the weed growers will love your industrial quality trich


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Registered: 01/01/20
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Re: flowhood, DIY, affordable, tested [Re: laughingdog] * 1
    #28184058 - 02/12/23 01:14 PM (11 months, 8 days ago)

Quote:

laughingdog said:
Via Gordo Tek

https://www.patreon....aminar-78502280

"This design passes the flame test as shown in the picture above, even when it's not turned all the way up to max flow rate. "----"I purchased an expensive Met One laser particle counter to test filters for this project, and PROVE that they were working as expected.  As far as I know, I am the first person to describe such a low cost laminar flow hood and also provide analytical testing showing that it works."----"I want to show video proof that the design works flawlessly, and I can tell you already that is the case as I've already made numerous agar dishes with a 100% success rate and even exposed open agar dishes to the flow for 5 minutes before closing them and waiting to see what would grow on them (nothing did!).  That combined with the Met One particle count results are pretty irrefutable proof."

I have not tried it, but would if my situation 'allowed' it

If I were to do it I would use a  hot knife cutting tool or hot knife cutting tool foam( from amazon) also listed under hot knife cutting tool plastic --as a Possible alternative to rotary cutting tool --May be less likely to crack the tote. If one does this, use outside because of fumes from melting plastic.---



Brought to you by the creators of Oven TEK, the most trusted name in sterile technique


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: flowhood, DIY, affordable, tested [Re: Moshe]
    #28190215 - 02/16/23 01:13 PM (11 months, 4 days ago)

Quote:

Moshe said:

Also the FFUs from amazon are so cheap now, i don't see any reason not to buy one. That is, if your goal is to grow them caps.



I don't have room for it and an SAB has served me very well.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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I'm a teapot User Gallery


Registered: 01/01/20
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u] * 4
    #28210987 - 03/02/23 12:17 PM (10 months, 21 days ago)

Question.

How do you seal glove box from bad stuff when you load in your jars and plates and tools?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap] * 3
    #28211228 - 03/02/23 03:59 PM (10 months, 21 days ago)

So I need to get argon or nitrogen? Oh FFS another damn thing to buy.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Registered: 01/01/20
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u]
    #28211293 - 03/02/23 04:41 PM (10 months, 21 days ago)

Quote:

Shroomin4u said:
You load them in the sealed box and use chemical sterilizer. (preferably the organism would be sealed in its own container as well. To allow it time for the sanitizer to settle.)

It settles to the bottom of the box, but unlike contaminants the very small amounts you move via your arms and hands don't do much. For one most sterilizers can be brute forced (Lets say a certain chemical binds to the proteins on a organisms membrane. Then if you have more organisms compared to this chemical its effects are muted.)

With containments even one very small organism can quickly multiply into several million in a matter of days. Chemicals don't multiply. They don't land in nutrients and divide and conquer. They're a linear problem vs a non linear answer.



What sort of chemical sterilizer would you recommend?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap] * 2
    #28211295 - 03/02/23 04:42 PM (10 months, 21 days ago)

Quote:

Stipe-n Cap said:
Don't forget the UV-C :kingcrankey:



Not sure what aliens have to do with this


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Shroomin4u] * 1
    #28211304 - 03/02/23 04:51 PM (10 months, 21 days ago)

Right but what chemical sterilizer do you recommend?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap] * 2
    #28211323 - 03/02/23 05:06 PM (10 months, 21 days ago)

:waitingpatiently:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 19 minutes
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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: myc_ousin_vinny] * 4
    #28211340 - 03/02/23 05:19 PM (10 months, 21 days ago)

Quote:

myc_ousin_vinny said:
I’m waiting for the glovebox demo video :popcorn:



I just wanna know what chemical sterilizer to get:shrug:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Unfilter Print Post Top
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