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Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs * 28
    #27615199 - 01/11/22 02:05 PM (2 years, 16 days ago)

Positive pressure boxes, Box fan flowhoods, Gloveboxes, and Automated Mono-Tubs.


Plenty of new folks have questions regarding equipment, their application and efficacy. From YouTube influencers, sub reddits, to Facebook and Instagram, there's no shortage of encouragement regarding these devices; let's take a moment to talk about these contraptions.

First:

Can one produce clean cultures while using any of the contraptions covered by this post? absolutely!

Cultivators with little to no experience can produce clean cultures in the open air without a box-fan flowhood, positive pressure box, shmuvbox, clean bench, SAB, or any other known cultivation tool. Humans have been growing mushrooms for thousands of years.

One can produce clean germination plates by shooting, or streaking multispore solution onto agar; clean spawn can be produced by spraying MS-solution liberally over cereal grains. It's incredibly easy to produce cultures or spawn, and by extension to grow mushrooms; anything can be achieved with a little luck.

We're not operating in a CDC clean room environment with virulent/pathogenic viruses or bacteria, cultivators can get away with an awful lot of shenanigans on a small enough scale.

Can you produce mushrooms while using a: shmuvbox, positive pressure box, boxfan flowhood, glovebox, etc? Yes!

Everything "works" so long as you possess the appropriate genetic starting materials like spores, or live mycelium, and a substrate upon which they will grow.

Cubensis will grow on manure, cigarette butts, bibles, fancy underwear, rags, etc. Just because you can, doesn't mean you should.

The topic of this thread covers the usage and theory relating to equipment employed by cultivators, not the statistical probability for rates of contamination while using them. Having said that, the statistical probability of failure is much higher while employing incorrect equipment or techniques.


Let's start with the appropriate equipment first, once those are explained things will make more sense when we go over the fundamental flaws of the others.

Laminar Flowhood/Fan Filter Unit (FFU)




A laminar flow cabinet/FFU utilizes velocity and geometry to force air currents into ordered lamina through a filter designed to efficiently remove particulate down to at least .3 microns. These filters are specifically designed for laminar flow applications.

Laminar flow cabinets are calibrated to produce a flow rate of 90-110 feet/min evenly across the entire face of the filter. This velocity will ensure stable flow without transitioning to turbulent flow.



This lamina has been arranged by the resistance generated by the HEPA filter (static pressure), the internal geometry of the filter, and the volumetric output of the blower.

The blower has been matched precisely to the surface area of the filter face, and to the filters resistance/flow rate @ 90-100fpm; this combination allows for sterile, turbulence free air to flow over your work area.









The turbulence generated by objects in flow (including your hands and tools) has the potential to pick up microscopic contaminant particles off of your hands, etc, and then deposit those particles into your work if you do not have great technique. Needless to say, these particles will produce mold.

Attention to detail is important when working with microorganisms, sterile work not only requires sterile media but proper maintenance of that sterility by minimizing exposure to potential contamination while working. Plan ahead of time where to place objects in flow; plan how you expect to move, where to place utensils; and how not to produce unnecessary turbulence by crowding your work area or by placing objects between the filter face and your work while moving.




Still Air Box






A still air box on the otherhand is an enclosed area that allows for particles to settle via gravity due to the absence of turbulent air currents that would otherwise keep them aloft, this is why they're called a "still air box".

Slow, calculated movements are required to prevent creating turbulence which will carry contamination into your work.



This is showing the time to settle in still air from a height of 1.5m; allow ample time for particles to settle in your still air environment.

Household dust typically consists of some combination of: human skin flakes and animal fur; decomposing insect parts; food debris; lint and fibers from clothes, bedding, and other fabrics; tracked-in soil; soot and ash; particulate matter from smoking and cooking; and allergens and pollutants from the environment. Climate also affects the composition of dust.

When operating your SAB: Turn off fans, AC or furnace ducts that disturb air currents within your space. Don't place speakers on top of your SAB; don't bang your SAB; don't place warm lights in contact with the top of your SAB; Keep your work elevated off of the floor of the box where the contsminant particles will settle; do not spray isopropyl alcohol inside of your box; do not flame sterilize instruments inside of your box.....don't disturb the air within the 'SAB' at all, up to and including pumping air directly into your SAB (filtered or not).

Use a SAB if you do not have an appropriate flow cabinet/FFU!


Glove Box



By securing gloves to the opening of your box you will be creating a vortex type turbulence within the box:






Gloveboxes create vortex type turbulence within an enclosed space, the frequency and amplitude of that turbulence is far greater than that of external air currents that interact with a standard SAB.

Don't make a glovebox:

"A glove box is a sealed container used to manipulate materials where a separate atmosphere is desired. They are commonly used to protect workers from hazardous materials or to protect chemicals and materials that may be sensitive to air or water vapor"

Glove boxes are not suited for microbiology, do not attempt to build or use one of these for cultivation.


Positive Pressure Box



As you can see these are SAB' outfitted with fans, the addition of fans will create a turbulent air box.

Positive pressure implies airflow into an enclosed area which means that this air regime will by definition become turbulent flow due to the geometry of the ducting and work area.

When turbulence is introduced into a system it creates turbulent eddies within the workspace:



These eddies will inevitably move particles (mold spores etc) around and into your work. This is no bueno.

No matter how clean your environment may appear to the naked eye, this is the reality:



Particles are suspended in open air as well as inside of your box. You are covered in particles, you shed particles produced by your skin, your hair, your respiratory system, etc.

SAB' work because you're allowing gravity to settle airborne particles that line/coat the plastic walls, floor, and ceiling of your tub, including any ductwork should you attempt to attach fans; this will remain true even if you were capable of evacuating all particles from the system due to the particles found on your arms, jars, bags, petri dishes, etc.

Introducing any gases, filtered or not, will kick these invisible particles back up into the air due to the velocity of the introduced gas and the internal geometry of the box.

Any air movement in a confined space will create turbulence/eddies.

Turbulence = contamination, no exceptions!

Not suited for microbiology, do not attempt to build or use one of these for cultivation.


Box Fan Flowhood/budget "flowhoods"



Check out the mold babies this guy created with his "flowhood"



"Gordo tek" is nothing more than a low quality filter matched to an over powered blower. This is not a flowhood:



1 fan + 1 filter doesn't equal anything other than mold.

Why doesn't this "budget flowhood" work? Well, remember that laminar flow is required which is the product of resistance, velocity, and geometry; furthermore the filter is not designed to filter out microscopic particles with high efficiency.

A blower attached to a filter, or set of filters, would be more aptly called a "blowhood". The particle sampling data clearly illustrates how ineffective the standard hardware store filters are when it comes to filtration efficiency.

3 reasons why a boxfan/blowhoods fail to mimic actual clean benches:

1. The velocity produced by a box fan falls well short of the minimum required to produce laminar flow;

2. Hardware store filters are not the sort of filter designed for high efficiency particle filtration, even if they were, not all HEPA filters are designed for laminar flow applications;

3. The filter does not produce enough resistance and does not possess the correct internal geometry to align the airlflow into lamina.

As a result you're left with a turbulent flow machine that sucks in contaminant particles, (maybe the filter catches some of the larger debris like cat hair, etc) but ultimately blows microscopic particulate all over your work area.


Quote:

Brewtality said:
I have some data to add to this thread.

I am not sure of the tek responsible for proper construction of a "Blowhood", but I went to Homie Depot and picked up the finest ingredients available on the shelf.

1 Lasko (Made in the USA) 20" box fan.
1 Honeywell 20"x20"x1" Elite Allergen Air Filter - FPR10
Roll of tape.

A quick run through the Gargler told me FPR10 is roughly equal to MERV13.

I ran a few tests using a Lighthouse Solair 3100 Particle Counter and these are the results.

This strictly evaluates the potential for contamination based on the number of particles in the air. Organisms that contaminate don't have feet, they don't walk into your plates, they ride on particulate.

The testing and method:
The samples were collected 12" from the filter face.
Each location was sampled 5 times and the results were averaged.
The sample duration was 1 minute.
The sample size was equal to 1 cubic foot of air.
All values represent particles 0.5 microns and larger.

Background Sample: Room Air Sample, no fan running (Aka Baseline)
High: 299,691
Low: 296,907
Average: 298,246 particles per cubic ft

Fan Sample: - Fan running on high with no filter
High: 284,209
Low: 260,840
Average: 270,921 particles per cubic ft

Fan with Filter: Speed setting 3 (High) with Filter attached.(Avg velocity 180 ft/min)
High: 61,895
Low: 29,653
Average: 39,734 particles per cubic ft

Fan with Filter: Speed setting 1 (Low) with filter attached. (Avg velocity 79 ft/min)
High: 20,190
Low: 18,577
Average: 19,571 particles per cubic ft

Chinese FFU Imported and marketed to the mushroom farmer: Speed Setting 3 (Average velocity 63ft/min)
High: 0
Low: 0
Average: 0 particles per cubic ft

The data above shows that using the method I have outlined the box fan filter fails to adequately reduce the particulate load when compared to a proper HEPA filter. You can decide if your time and money are worth the risks.




Particles sampled using this equipment:

https://www.golighthouse.com/en/airborne-particle-counters/solair-3100

Brewtality certifies clean benches and clean rooms for ISO classification.

Common hardware store filters do not efficiently reduce airborne particle concentrations, they are not designed for laminar flow nor do they produce laminar flow; this class of filters will produce particles. 


Not suited for microbiology, do not attempt to build or use one of these for cultivation.


Automated Mono-Tub




Quote:

au·to·ma·tion

noun

the use of largely automatic equipment in a system of manufacturing or other production process.




Mono-tubs are equipment that are used for the production of mushrooms, this equipment, despite lacking electricity or moving parts, is automated. Mono-Tubs are 100% self contained, self sufficient, self regulating, self humidifying, fresh air exchanging environments.



By properly hydrating your substrate at an appropriate depth you are creating a moist/humid environment, this environment will maintain the appropriate humidity throughout the fruiting cycle providing that there isn't excessive air movement in the room you choose to house them in. No need for fanning, no need for misting.

The holes on the walls of the tub allow for warm air created by your culture to travel up and out of the holes situated at the top of your tub, as well as the gaps between your lid (so long as the tubs aren't stacked); as the warm air exits through the top holes as fresh air comes in through the holes situated at substrate level. This is passive automation, mono-tubs are designed to not require active automation. The addition of automated humidifiers and fresh air exchangers actually inhibits the efficiency of microclimate to regulate itself.

I personally prefer modified tubs (tubs with holes drilled into the ends below the handles and sides above the substrate level) for this reason: they can be stacked;

Unmodified tubs (tubs without holes) lose airflow when stacked for obvious reasons (the lid gap is closed when stacked which prevents airflow ; ) )

I have never once saw a tub automated by the addition of some contraption out-perform a dialed mono, and neither have you.

Don't use automated mono-tubs.


UV light's


Ultraviolet germicidal irradiation (UVGI) is a disinfection method that uses short-wavelength ultraviolet (ultraviolet C or UV-C) light to kill or inactivate microorganisms by destroying nucleic acids and disrupting their DNA, leaving them unable to perform vital cellular functions

Ultraviolet lights are 100% unnecessary, this equipment has no application for the production of agar plates, liquid media or spawn.

1. Sterilization is required for media and grain spawn, why attempt to use two methods of sterilization when steam sterilization is the most versatile and effective of the two?

2. UV light cannot penetrate grain, this should be self evident, this is why we utilize autoclaves and pressure cookers;

3. Pressure cookers/autoclaves destroy bacterial endospores, UV does not compare:

Bacillus endospores are 5–50 times more resistant to UV radiation than their corresponding vegetative cells

https://academic.oup.com/femsec/article/51/2/231/503139

4. Agar and other liquid media is sterilized using autoclaves/pressure cookers, it would be pointless to utilize UV to sterilize groups of individually poured petri dishes;

5. UV lights will not sterilize the "air" within a glove box, positive pressure box, SAB, etc.

Ultraviolet lights are pointless, do not attempt to use these tools for the purposes of producing clean cultures.
Steam sterilization is effective and safe., UV is not.

UV has no practical application for  mushroom cultivation.

https://www.ehs.washington.edu/about/latest-news/trouble-uv-light-your-biosafety-cabinet


Ozone

When inhaled, ozone can damage the lungs. Relatively low amounts of ozone can cause chest pain, coughing, shortness of breath and, throat irritation. It may also worsen chronic respiratory diseases such as asthma as well as compromise the ability of the body to fight respiratory infections.

Just don't use ozone, please...smh :facepalm3:

Adopting poor habits, using the incorrect tools or procedures does not make you a pioneer or a trailblazer, it makes you a noob.

Use the right tools for the right job, employ the proper procedures and techniques.








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Re: Box fan flowhoods, glove boxes, Positive pressure boxes. [Re: sandman420] * 2
    #27615239 - 01/11/22 02:28 PM (2 years, 16 days ago)

I figure it falls under the same umbrella, but I can add the FFU part to the heading.

And done.


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Box fan flowhoods, glove boxes, Positive pressure boxes [Re: Land Trout]
    #27615386 - 01/11/22 04:51 PM (2 years, 16 days ago)

Thanks man.


Edited by Stipe-n Cap (01/12/22 01:34 AM)


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Stipe-n Cap] * 5
    #27711233 - 03/28/22 06:56 AM (1 year, 9 months ago)

Bumping to remind you kids to stop buying dumb shit.:lol:


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: CowsPoopShrooms]
    #27812031 - 06/09/22 10:22 AM (1 year, 7 months ago)

I'm glad that you've found it useful; once you know the why you'll simultaneously know the how in many instances.

Cultivation is quite simple once you understand a few basic principles which will apply to nearly every situation.

A firm grasp of the basic principles will fine tune your bullshit detector so that you won't have to build a "boxfan-flowhood" for yourself to understand why it isn't practical or advisable.


Edited by Stipe-n Cap (09/14/22 06:31 PM)


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: mushboy]
    #27812107 - 06/09/22 11:28 AM (1 year, 7 months ago)

; )


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: ruawakeyet] * 1
    #28006171 - 10/19/22 10:04 AM (1 year, 3 months ago)

That's part of the intention with this thread, to clarify what and why.

The SAB portion of the OP clearly states that SAB is not only an acronym for still air box but includes the details describing why still air is necessary.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: LiquidGlass] * 4
    #28018916 - 10/27/22 08:39 AM (1 year, 2 months ago)

Quote:

LiquidGlass said:
I use a ghetto boxfan "flow hood" and I have great results using it for agar and grain transfers. So, while it is not ideal, there is a way to use that one successfully





I hate to break it to you but the a science is not up for debate, it simply isn't.

In this case a great result would mean that you actually achieved laminar flow with your build, did you? No, you didn't.

What you mean here by "great result" is that despite the shortcomings of the design features of your contraption and the gaps in your understanding you managed to produce some mushrooms.

Producing plates, grain jars, or mushrooms of any quality whatsoever is not a sufficient condition for claiming that any given procedure, technique, or piece of equipment is a great success; it is a necessary condition, but not a sufficient condition.

On a small enough scale much can be achieved even with a fan blowing mold spores at you, much can be achieved with incomplete sterilization, septic technique, septic initial conditions, etc.

The fact that one can produce mushrooms without the proper equipment; starting conditions, materials, or techniques isn't a testament to the efficacy of those practices or materials so much as it is the reality of statistical probability and the hardiness of the organism.

Can you produce cultures and mushrooms without fancy lab equipment: yes

Can you produce the same using duct tape, cardboard and a complete misunderstanding of microbiology and physics: yes

To say that any septic technique or procedure is legitimized by the production of mushrooms is a type 1 error.

Mush Cult is one of the few hobby communities where folks line up to receive trophies for a job barely done.


Edited by Stipe-n Cap (10/27/22 04:02 PM)


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: LiquidGlass] * 1
    #28021326 - 10/28/22 12:35 PM (1 year, 2 months ago)

Listen, man, this thread topic is related to the fundamental mechanics  of laminar flow, still air, and why they are preferable to all other half assed methods.

If you have a "cheap" set up that produces laminar flow then good for you; nobody, including myself is going to entertain cardboard boxes assembled with duct tape no matter what you have to say.

If that's not what you have, then say so, don't go bating with ambiguous commentary.

This thread will not turn into misguided arguments in favor of the very things this thread was designed to discourage, and they're to be discouraged for good reason.

It was o ly a matter of time before someone got butt hurt and posted about the grand efficiency of box fan flowhoods or automated mono-tubs, UVC, etc, etc.

If you want peer reviewed material then go find it, it's not like I engineered the clean bench and have foisted laminar flow on the community, there is good reason why you will never see this is in virology or microbiology laboratory:



If only the commercial farms new about this, the money they would save on purchasing or building expensive cabinets, bench', etc.

Why not just make a wall of laminar flow out of empty refrigerator boxes and call it a day.


Carry on.


Edited by Stipe-n Cap (10/28/22 12:51 PM)


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: LiquidGlass] * 1
    #28021361 - 10/28/22 12:55 PM (1 year, 2 months ago)

Quote:

LiquidGlass said:
I'm not but hurt at all.  I am just commenting for the people that are wondering are on the fence if they can do it for less money or not. And for clarity,I would guess it's more in between a positive pressure box/boxfan flowhood. And I didn't use cardboard.
And for the people wondering, ignore the haters and elitists and just know that you actually can construct a decent flowhood with a boxfan. I have gone through around 250lbs of grain transfers using this with less than 1%contam rates. Don't let people discourage you from cheap diy stuff if it works efficiently




Are you serious, 250 pounds! Wow, that sure is a lot of grain.:lol:

Like I said before and I will say it again:

ANYTHING is possible at small scale!

Laminar flow and still air are not elitist. Let me guess, did you use an instant pot to process all 250lbs or....

Let's keep this thread on topic, you're making it extremely difficult to gate keep
:rofl:


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: ruawakeyet] * 2
    #28021419 - 10/28/22 01:22 PM (1 year, 2 months ago)

Ok let's rein things in here.

Congratulations on your cultivation success using less than ideal methods, nobody ever once said that you cannot produce mushrooms this way, or any other less than ideal way; if you are happy with your results and process then that is excellent, well done.

I am not going to allow this thread to be highjacked by turbulent flow/positive pressure box hybrid enthusiasts.

There is absolutely no argument against still air or true laminar flow; fluid mechanics is a thing, physics is a thing, this has been clearly articulated in the OP:

Turbulence = contamination. Full stop.

Thanks.


Edited by Stipe-n Cap (10/28/22 03:14 PM)


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: LiquidGlass] * 2
    #28021631 - 10/28/22 03:08 PM (1 year, 2 months ago)

Quote:

LiquidGlass said:
It may not be as efficient as an expensive setup but less than 1% contamination rate is  very good for any grower.




This is not a supported claim.

I have no idea who you are, I cannot confirm nor deny the veracity of the claim, I cannot confirm your ability to identify contamination, I cannot confirm that the pictures are yours or if they were actualy grown out using the equipment that you've mentioned.

What I do know is that the science is known, the mechanisms are well defined and understood; these practices are inherited from the scientific community at large.

To be on topic here we would be engaging in discussion about WHY your equipment should work, what are the mechanisms/principles that are responsible for the effective deployment of this equipment against airborne particles. The equipment in question should have a clear casual relationship between materials and expected outcome, this needs to be clearly articulated.

Why should your equipment work? We know why laminar flow and still air work, we understand why turbulence should be avoided and how; There is a clear causal relationship between turbulence and contamination.

Making unfounded claims that upend decades of scientific inquiry will require more information.

If you are unable to supply this information then it's because you are unaware of why it should work. If there is no clear explanation why it should and does work then the likelyhood that your results are a false positive exponentially increase and may simply be the result of chance, which is a type 1 error as mentioned in my previous reply.

If your equipment is incapable of achieving laminar flow or still air then you must supply a reasonable replacement, otherwise your anecdotal experience is just that, left to chance, statistically insignificant across time and not repeatable/falsifiable.

Type 1 error tek.


Edited by Stipe-n Cap (10/28/22 03:36 PM)


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: ruawakeyet] * 2
    #28021682 - 10/28/22 03:41 PM (1 year, 2 months ago)

What an elitist :lol:

Quote:

LiquidGlass said:
There is no way you can say it's a turbulent airbox without actually seeing the design and how it flows




So what you're saying is that you're actually using laminar flow?:facepalm3:

So what is it then? Laminar flow or chaotic turbulent flow, there are only two choices here.

You've highjacked my thread to send out a PSA to noobs to not listen to the elitist dogma found in this thread, that we won't accept "alternative" methods; all I'm asking you for is clarity.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: LiquidGlass] * 2
    #28021704 - 10/28/22 03:56 PM (1 year, 2 months ago)

Quote:

LiquidGlass said:
There is no way you could know how the air is flowing in my contraption




I will ask you again,

Is your equipment designed for laminar flow or not?


Quote:

LiquidGlass said:
I am just commenting for the people that are wondering are on the fence if they can do it for less money or not. And for clarity,I would guess it's more in between a positive pressure box/boxfan flowhood. And I didn't use cardboard.
And for the people wondering, ignore the haters and elitists and just know that you actually can construct a decent flowhood with a boxfan.





Laminar flow is the result of geometry, velocity, and resistance. I can most certainly determine if the unit is capable of laminar flow just by looking at a build or by reading the details of it's design features.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: ruawakeyet] * 1
    #28021719 - 10/28/22 04:12 PM (1 year, 2 months ago)

He doesn't have an explanation for why his design should be effective against airborne particles, he won't explain if his design is capable of laminar flow or not; he has nothing to contribute other than to warn noobs against elitist dogmatism.

Thanks for your contribution.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: Brewtality] * 1
    #28022127 - 10/28/22 09:25 PM (1 year, 2 months ago)

I will certainly add this to the OP, excellent work; thank-you very much for the contribution. Top notch data.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: LiquidGlass] * 2
    #28022531 - 10/29/22 08:54 AM (1 year, 2 months ago)

Quote:

LiquidGlass said:
... However the boxfan flowhood is not something I've been using for a long time. I only adopted this method in the past couple months, so it's not about sticking to old methods for me.




This matter is now closed. Success with too small of a sample size will produce false positives. Failure will accrue over time, the materials are not capable of particle filtration nor laminar flow. The hard data and theory are complimentary and irrefutable.

It stands to reason that if we make the effort to produce sterile media then we should maintain that sterility by employing the appropriate techniques and equipment. We have 2 options at our disposal:

1. Laminar flow;
2. Still air. 

The OP clearly explains the why and how, we now have the further benefit of particle measurements to accompany that information. Particles and the turbulence that carries them produce contamination.

Quote:

Bertrand Russel said:
“You may reasonably expect a man to walk a tightrope safely for ten minutes; it would be unreasonable to do so without accident for two hundred years.”





The same applies to spawn production.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: SamuraiJACsr]
    #28033904 - 11/05/22 07:15 AM (1 year, 2 months ago)

If you have holes in your tub then you don't need to do anything else to the tub for fresh air exchange; the humidity sensor is unnecessary but it won't hurt anything.

Still air boxes are employed for sterile work, not for use with mono-tubs. Tubs are spawned in open air.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: PsillySeeEms]
    #28049010 - 11/13/22 02:50 PM (1 year, 2 months ago)

It's a shmuv box, I personally don't recommend anything that isn't still air or laminar flow as outlined in the op.


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Re: Box fan flowhoods, Glove boxes, Positive pressure boxes and Automated Mono-Tubs [Re: PsillySeeEms]
    #28049116 - 11/13/22 03:43 PM (1 year, 2 months ago)

Any SAB will be better than some rando contraption; the simplicity and effectiveness of still air outweighs the risk associated with contraptions other than appropriate laminar flow cabinets.

There are no real shortcuts long-term.


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