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OnlineLand TroutM
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Re: The Official Semilanceata Thread [Re: Baba Yaga]
    #27641095 - 02/01/22 03:08 PM (1 year, 11 months ago)

:rockon:
Thank you for this legwork, thank you a lot!


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: Land Trout] * 1
    #27641137 - 02/01/22 03:51 PM (1 year, 11 months ago)

Yeah no worries, I love doing this stuff.

Having the feeling that they will do alright on most if not all of these substrates since they are being already on steroids from all the nutes
in the grain spawn. 100% coir compared to the rest is what I'm mostly interested in cause it has no decomposed plant matter in it.


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InvisibleMelliferous
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Re: The Official Semilanceata Thread [Re: Baba Yaga]
    #27641370 - 02/01/22 06:27 PM (1 year, 11 months ago)

I've got an old semi print from Spain - going to follow along and see if I get inspired enough to see if they'll germ. :smile:

Thanks for all the trailblazing sir!


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"No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."


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OfflineAlan RockefellerM
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Re: The Official Semilanceata Thread [Re: Melliferous] * 9
    #27651744 - 02/09/22 02:10 AM (1 year, 11 months ago)

I did some microscopy on fruits cultivated by smalltalk_canceled, here are my findings:

Spores measure (11.5) 12.2 – 13.7 (16.5) Γ— (6.9) 7 – 7.6 (8.6) Β΅m
Q = (1.6) 1.7 – 1.89 (1.9) ; N = 30
Me = 13 Γ— 7.3 Β΅m ; Qe = 1.8

Spores 1000x



Cheilocystidia 1000x



Behind the scenes: 



Today a few people visited my lab including Peter Werner, who is a professional microscopist and a coauthor on the paper that described Psilocybe allenii.  We decided to study this sample, and found that microscopically it's a perfect match for Psilocybe semilanceata.  Peter made the cheilocystidia mounts and I was able to get some really nice photos of them.  The cheilocystidia in this species is very distinctive, and we don't think there's any chance that it's something else.


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: Alan Rockefeller] * 1
    #27651760 - 02/09/22 02:57 AM (1 year, 11 months ago)

Awesome news Alan, thank you very much!


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Offlinesmalltalk_canceled
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 10
    #27651968 - 02/09/22 08:15 AM (1 year, 11 months ago)

Haha , it's official.

Take lunch, disbelievers


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Willpower is the one true virtue



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OfflineAdas
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Re: The Official Semilanceata Thread [Re: smalltalk_canceled] * 3
    #27652027 - 02/09/22 09:10 AM (1 year, 11 months ago)

We are happy for you, Smalltalk! It's great success for sure. :cheers:


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OnlineLand TroutM
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Re: The Official Semilanceata Thread [Re: smalltalk_canceled] * 4
    #27652063 - 02/09/22 09:43 AM (1 year, 11 months ago)

How simple can it be, or how complicated can we make it?  Wish I had some from you collection on agar smalltalk, but was waiting for that confirmation.  It will be neat to compare it with my other cultures. 
One really low effort experement I was running might be interesting.  I had one plate the had some bacteria show up, so it went on the discard shelf.  I had a hay bale that was pretty shitty, sometimes my sheep are either spoiled or they bale is just too stemmy for them, so I just left this bale outside all winter.  It’s sprouted, moldy, and colonized with inky caps.  I cut a big handful from the bale put it in a unicorn bag pasteurized it and put the semi plate with the bacteria on it inside the bag.  And the fungi jumped from the plate to the spoiled hay.  It was slow going but was going about as fast as I’m observing on grain.  I also added a little bit of dry coir to the bag to balance the moisture content.  I know it’s not a huge breakthrough, but gets the wheels turning for possible future experiments.  I’ll try this with some more control shortly.  Oh, almost forgot the important part.  I put it outside by a big clump of grass and it looks like it died.😿


Edited by Land Trout (02/09/22 09:50 AM)


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Offlinesmalltalk_canceled
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Re: The Official Semilanceata Thread [Re: Land Trout] * 4
    #27652209 - 02/09/22 11:42 AM (1 year, 11 months ago)

I have prints for any shroomery member interested, first come, first served.

Looking for Bohemica and Baeocystis, but no requirement


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Willpower is the one true virtue



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Invisiblemind.at.large
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Re: The Official Semilanceata Thread [Re: smalltalk_canceled] * 1
    #27652340 - 02/09/22 01:16 PM (1 year, 11 months ago)

Smalltalk that is dope news!! Way to go dude super awesome!


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InvisibleMysticMycologist
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Re: The Official Semilanceata Thread [Re: mind.at.large]
    #27652363 - 02/09/22 01:35 PM (1 year, 11 months ago)

And so it begins.


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: MysticMycologist] * 3
    #27652592 - 02/09/22 04:53 PM (1 year, 11 months ago)

Almost done with spawning.

I've done 3 different, smaller outdoor patches instead of one big patch. From left to right the substrates are bagged compost, partially
composted grass clippings and the last one is just soil that was in there since last year.




Also a few planter pots, three with compost coir, one with potting soil right out of the bag and the shoebox is compost coir as well
which I had colonizing inside but started to show a bit of the mean green.




In total 2 shoeboxes and 1 minimono have shown either green or pin mold. I hope this is not culture related, have used 4 different LCs to
noc up 20 jars so I doubt that all of them will turn sour, could have gotten in during noc-up as well.

All outdoor substrates will get some grass on top, not that I think it will help because of some symbiotic relationship, it's simply because
it's so windy and dry here at times that I need something growing on top to keep things from drying out when I'm out of town.



The cakes are almost all done, will let those consolidate for another 3-4 weeks before putting in the ground. Lost 5 out of 21 early on.




And the jars that were inoculated over 4 month ago. Lost 4 out of 11 early on.




One spawn jar left gonna use this one to replace the mono tub I lost.


Edited by Baba Yaga (02/10/22 12:29 PM)


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 2
    #27653819 - 02/10/22 01:41 PM (1 year, 11 months ago)

Just checked on the two jars I cased and left with the lids on loose. Was putting them in the fridge every now and then at the beginning but
this didn't do anything so I just kept them in the cupboard and misted every now and then.

Now we had quite high temps for a week in the mid to high 80's and I went to mist the jars since I haven't looked at them for 3 weeks or so.
Found one of the jars with what seems to be lots of knots. Gave this one a good mist and will be putting it in the fridge for a couple of days or more.

I hope this is what I think it is.



Edited by Baba Yaga (02/10/22 01:58 PM)


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InvisibleMelliferous
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Re: The Official Semilanceata Thread [Re: Baba Yaga]
    #27653832 - 02/10/22 01:47 PM (1 year, 11 months ago)

:popcorn:


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"No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."


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InvisibleTormatoMFacebookDiscordReddit
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Re: The Official Semilanceata Thread [Re: Melliferous] * 1
    #27654103 - 02/10/22 05:34 PM (1 year, 11 months ago)

Well....you got my attention for sure.

:sneakypeek:


Just happen to have 2 lib prints :rockon:


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OfflineYeetusdeetus
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Re: The Official Semilanceata Thread [Re: Tormato]
    #27654254 - 02/10/22 07:15 PM (1 year, 11 months ago)

:threadmonitor:


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InvisiblerhizoRider
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Re: The Official Semilanceata Thread [Re: Yeetusdeetus]
    #27654328 - 02/10/22 07:58 PM (1 year, 11 months ago)

:popcorn:

I wish wxom ( spelling) would post back also with agar plate looking like enigma cubes!
Does anybody remember that agar plate cluster pic?πŸ“


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Offlinesmalltalk_canceled
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Re: The Official Semilanceata Thread [Re: rhizoRider] * 1
    #27654699 - 02/11/22 03:28 AM (1 year, 11 months ago)

Yep I saw. But primordia on agar plates is commonplace for me, the fruits won't mature though so far.


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OnlineLand TroutM
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Re: The Official Semilanceata Thread [Re: smalltalk_canceled]
    #27654842 - 02/11/22 08:24 AM (1 year, 11 months ago)

How long have your grains taken to colonize.  I got two quarts that were knocked on the 10th of January.  They’re looking good, but god damn, still were only about 75% the other day.


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: Land Trout] * 1
    #27655377 - 02/11/22 04:12 PM (1 year, 11 months ago)

The jars took 4-5 weeks to fully colonize with temps being around ~75F (~25C). I noced up with LC and had PF-Tek substrate on top of the
grain to make sure the LC recovers fast. This might have made a difference. Shook them twice.



So far the my cultures have mostly been OK on agar and grain in terms of grow speed but I read a few times that they can be slow.


Edited by Baba Yaga (02/11/22 04:18 PM)


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