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InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: The Official Semilanceata Thread [Re: scapo] * 1
    #28537246 - 11/10/23 02:08 PM (2 months, 16 days ago)

Quote:

scapo said:
Hey all! I recently succeeded with my journey cultivating libs and wanted to share. I wrote in a journal here but will copy it here as well: https://www.shroomery.org/forums/showflat.php?Cat=0&Number=28537157&page=0&vc=1#28537157

Jan 2023: obtained a spore print from a wild fruit in Sweden

Prepared agar and after 2 weeks, it germinated nicely. I did a single agar transfer, and from that T1 plate, I prepared a small 8oz LC jar from the mycelium. My LC has LME and peptone.

Once that was colonized, I inoculated a small 8oz BRF cake (replacing rye flour as it's all I had around). That took over a month to fully colonize, these cakes are definitely slower than grains.

Come May 2023, I prepared a 5gal fabric pot with the following:

- Happy frog soil (~55%)
- Vermiculite(~20%)
- Coco (~10%)
- Alder smoking chips (~10%)
- Worm castings (~5%)

These were all mixed into a tote, the pot was filled half way, then the colonized cake was broken up into the center of the pot. The pot was filled up to 90%, then perennial rye grass was planted into half the pot. The pot was topped off and left outside throughout the summer getting top & bottom watered like a normal plant. I watched the grass to make sure it was alive.

Once the temperatures dropped below 60F at night at the end of August, I got pins!



I left them to do their thing and had mild challenges with animals ripping them out but overall they performed very well. They fruit continuously and as of today they're still fruiting!































These have been sequenced and are confirmed Psilocybe semilanceata. They tested pretty high baeocystin as well.







Oh yes, those are beauties. Just amazing how they develop such thick stems when cultivated. Their divers looks can be really mind boggling.

Thanks for sharing.


Edited by Baba Yaga (11/10/23 03:50 PM)


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OfflineFungus Gnat
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Registered: 11/08/22
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 1
    #28537419 - 11/10/23 04:07 PM (2 months, 16 days ago)

:popcorn:
I saw this pop up on inat a week or two ago, seriously nice work on that scapo. Awesome to see all the headway on this species.


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Offlinescapo
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Registered: 11/05/22
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 2
    #28537906 - 11/10/23 10:14 PM (2 months, 15 days ago)

I am almost positive it has to do with the addition of wood chips. Wood grown libs are thick!


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Offlinescapo
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Re: The Official Semilanceata Thread [Re: Fungus Gnat]
    #28537907 - 11/10/23 10:15 PM (2 months, 15 days ago)

Thank you! I appreciate it, I was surprised I ended up with fruits as fast as I did and I'm fortunate I was able to get sequencing and HPLC data relatively quickly with the help of some friends


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Invisiblealaskappalachian
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Registered: 10/22/19
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Re: The Official Semilanceata Thread [Re: scapo] * 4
    #28540578 - 11/12/23 04:15 PM (2 months, 14 days ago)



Transferring in a few from the first three of maybe 9 cultures I was cleaning up on this water agar.    Can't wait to get through the grain spawn hurdles stage eith these again :lol:  Nice pic porn, scapo.


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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Offlinegoatboy666
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Re: The Official Semilanceata Thread [Re: alaskappalachian] * 2
    #28548750 - 11/19/23 05:52 AM (2 months, 7 days ago)



I had some on agar a few years back but was unsure if I’d picked the correct shroom…. Anyway, got some this year and it grew like the old stuff….what is weird is that the mycelium seems to grow into the agar as well as the surface? Anyone noticed this?


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Offlinescapo
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Re: The Official Semilanceata Thread [Re: goatboy666] * 1
    #28549007 - 11/19/23 10:16 AM (2 months, 7 days ago)

Yes, some species will even grow more inside the agar with minimal surface growth.

I see this in libs, pelliculosa, baeocystis, and several other exotics but those are the ones I see it in consistently


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Offlinegoatboy666
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Re: The Official Semilanceata Thread [Re: scapo]
    #28549400 - 11/19/23 04:48 PM (2 months, 7 days ago)

Great πŸ‘πŸ» that’s helpful to know


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InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
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Re: The Official Semilanceata Thread [Re: goatboy666] * 4
    #28558633 - 11/27/23 05:36 PM (1 month, 30 days ago)

Have revived the last of the LCs I made last year and put some to PF-pucks.

The mycelium is looking nice on it. This is T2 counting from LC.





and T3 on agar




It seems the growth has lost that pube-curliness I am used to see, might be coming back later though.


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OfflineTweeq
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 5
    #28563649 - 12/01/23 11:24 AM (1 month, 26 days ago)

Most here will already have plenty prints I guess but I'm giving away some fresh ones from the NL here:

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=28563586&page=0&vc=1#28563586


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OfflinePsilocyberino
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Re: The Official Semilanceata Thread [Re: Tweeq] * 2
    #28593650 - 12/22/23 06:00 AM (1 month, 5 days ago)

Hello! I'm completely new here but decided to chip in with my own experience and also questions.

I'm from Switzerland and collected my own P. semilanceata spore print. I will attach pictures to how my plate looked like after 1 week and after 2 weeks of scraping a spore print on my agar plates.

By the way, I came here solely from scapo's amazing posts from Reddit!! Congratulations to your awesome work and documentation




Edited by Psilocyberino (12/22/23 06:06 AM)


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Offlinescapo
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Re: The Official Semilanceata Thread [Re: Psilocyberino]
    #28593922 - 12/22/23 10:35 AM (1 month, 5 days ago)

Ayyy I appreciate it!

That looks great so far, the one spot does look contaminated but nothing to worry about. I would take a transfer from spots that look like they have multiple germinating spots next to each other so you increase your chances of getting a dikaryon, and from a distance away from those center suspicious looking one.

How long did these take to germinate?


Edited by scapo (12/22/23 10:43 AM)


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OfflinePsilocyberino
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Re: The Official Semilanceata Thread [Re: scapo] * 1
    #28600491 - 12/28/23 04:40 AM (30 days, 17 hours ago)

Thanks scapo! So I checked again and the first picture was taken exactly 7 days after scraping spores onto the plate and the second picture was taken 13 days after. The plate is rich Yeast Malt extract media.

I made multiple transfers a week ago and I'm looking forward to replicate what you did in spring. :laugh:


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Offlinescapo
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Re: The Official Semilanceata Thread [Re: Psilocyberino]
    #28600658 - 12/28/23 09:17 AM (30 days, 12 hours ago)

I'm looking forward to seeing your progress!


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Invisiblealaskappalachian
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Re: The Official Semilanceata Thread [Re: scapo] * 3
    #28602546 - 12/29/23 06:29 PM (29 days, 3 hours ago)

Fucks sake... finally got a clean jar of semi spawn again.  The first hard part is over :lol:  I still can't find a rhyme or reason for selecting semi myc.  My plates that fail look flat and featureless when they age and the ones that succeed age like
:shrug:  Think my other WBR jar will be clean too as long as it recovers from yesterday's shake.  G2G time :lookslucrative:

Edit: the green is a reflection from my weed container...


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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Offlinegoatboy666
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Re: The Official Semilanceata Thread [Re: alaskappalachian] * 3
    #28610499 - 01/05/24 01:01 PM (22 days, 9 hours ago)



Going to try a pot grow. Suprisingly didnt have any contam issues with a wild print. Very slow growing mycelium


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Offlinesmalltalk_canceled
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Re: The Official Semilanceata Thread [Re: goatboy666] * 4
    #28618998 - 01/12/24 05:54 PM (15 days, 4 hours ago)

4 substrate mix tubs being given the fimetaria treatment in a freezing to 10c garage.


--------------------
Willpower is the one true virtue



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Offlinescapo
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Re: The Official Semilanceata Thread [Re: smalltalk_canceled]
    #28619629 - 01/13/24 11:12 AM (14 days, 10 hours ago)

Hell yea! Loving the updates!


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: scapo] * 4
    #28620269 - 01/13/24 08:57 PM (14 days, 1 hour ago)

Inoculated 4 LC broths today.

One of the donor plates.



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OfflineTweeq
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 4
    #28620576 - 01/14/24 06:54 AM (13 days, 15 hours ago)

Nice!

As it happens I also inoculated some lc broths just now :smile: Had a bunch of plates to choose from and 6 of them looked slightly better than the others.



Hopefully lc will also speed things up a little bit. I find Semi myc to be quite slow on agar (1.4pct lme).


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