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OfflineMycomatic
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Registered: 12/30/21
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Potency Analysis on Major and Minor Hydroxy-Tryptamines * 1
    #27600193 - 12/30/21 06:13 PM (2 years, 4 months ago)

Hi everyone.

Link to Article

This is a short article defining potency analysis on major and minor hydroxy-tryptamines including:
  • Psilocybin
  • Psilocin
  • Baeocystin
  • Norbaeocystin

All the information is theoretical. With that said, the article opens up the conversation for more in depth discussions on the chemical composition of psilocybe cultivars.

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OfflineAlan RockefellerM
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Re: Potency Analysis on Major and Minor Hydroxy-Tryptamines [Re: Mycomatic] * 1
    #27600614 - 12/31/21 12:17 AM (2 years, 4 months ago)

Interesting how they tested small, medium and large Avary Albino - the smaller ones were only slightly more potent, indicating that they should definitely be left to mature to full size for maximum psilocybin.

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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,230
Loc: Floating in liquid gardens
Re: Potency Analysis on Major and Minor Hydroxy-Tryptamines [Re: Alan Rockefeller] * 1
    #27601021 - 12/31/21 10:23 AM (2 years, 4 months ago)

This is interesting. I’m glad to see more testing being done as it’s amazing to see just how much variability in alkaloid production there is within this species. But I’m wondering if any recent tests have been done on other psilocybin producing species, given that these newer tests show numbers way higher than previously recorded, at least when referencing that old chart from a while ago that put azurescens on top.

But I’m wondering if anyone now knows what else goes into determining potency levels, besides just genetics. I remember reading an article a few months ago showing highly varied levels within a monoculture. If potency levels vary within the same genetics, then there has to be some sort of environmental conditions that can manipulate alkaloid production.


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OfflineMycomatic
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Re: Potency Analysis on Major and Minor Hydroxy-Tryptamines [Re: Alan Rockefeller]
    #27605308 - 01/03/22 02:54 PM (2 years, 4 months ago)

That's definitely a great point!

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OfflineMycomatic
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Re: Potency Analysis on Major and Minor Hydroxy-Tryptamines [Re: mind.at.large]
    #27605328 - 01/03/22 03:06 PM (2 years, 4 months ago)

Quote:

But I’m wondering if any recent tests have been done on other psilocybin producing species, given that these newer tests show numbers way higher than previously recorded, at least when referencing that old chart from a while ago that put azurescens on top.




I'd love to see a link that old chart.

With that said, the higher data points could be an indicator that the testing method, LCMS (Liquid Chromatography Mass Spectrometry) provides more accurate results, or that genetics i.e. potency is stronger in present day cultivars.

Quote:

But I’m wondering if anyone now knows what else goes into determining potency levels, besides just genetics.




Environmental conditions likely do play a major part in potency. When growing outdoor cannabis, plants within the same crop could yield varying degrees of cannabinoid content depending on soil content, sunlight, etc in a specific area.

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Invisiblemind.at.large
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Re: Potency Analysis on Major and Minor Hydroxy-Tryptamines [Re: Mycomatic]
    #27605882 - 01/03/22 07:54 PM (2 years, 4 months ago)

https://www.erowid.org/plants/mushrooms/mushrooms_info4.shtml

That’s the old chart that I’m talking about. The new tests, like in the op and the Oakland hyphae ones, demonstrate a significantly higher tryptamine level in cubensis, which I’m not surprised by. Obviously us growers have been selecting for potency over the decades, so, like cannabis, we will end up with more potent varieties. But I doubt that much genetic manipulation has happened in the less frequently cultivated species so I’m interested to know what modern tests will say about their levels.


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OfflineTri-Polar
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Re: Potency Analysis on Major and Minor Hydroxy-Tryptamines [Re: mind.at.large]
    #27607581 - 01/05/22 05:13 AM (2 years, 4 months ago)

I wonder if the hydroxy-tryptamines really have a noticeable effect when they are at less than 100 times the concentration of total psilocin

I've seen people argue these compounds could be responsible for a varieties "trip profile" but im not convinced at all, it seems like it mostly just comes down to more or less psilocin


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