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O0ZE
Ascended Drone for the hive
Registered: 02/28/18
Posts: 321
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screwed up agar? no. spores were just slow to grow.
#27596624 - 12/27/21 06:17 PM (2 years, 2 months ago) |
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Nothing will grow on the Agar I made because I didn't first come here to run it by you guys. wa wa wa. it's made with 500ML water of boiled sweet potatoes and oats. followed the cooking and sterilization steps here: https://www.shroomery.org/forums/showflat.php/Number/18431006
did not do yeast, or malt extract. seems as though my base liquid as it were is useless. I think I should unfreeze what I saved to add malt extract and yeast. OR just throw the base out since nothing grows on it anyways... what should I do?
Also, I'm looking for a microscopy hobbyist point of contact. I would like to study some spores under a microscope. I'm currently studying Cubensis A+ and Golden Teacher. fascinating. Please DM if looking for a hobby buddy. I would also like to study the peyote seed's structure if that's legal. I live in Southern California.
tell me I'm a dumbass, and how to fix my agar please
Edited by O0ZE (01/26/22 10:01 PM)
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MysticMycologist
Dirt Sherpa
Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE]
#27596628 - 12/27/21 06:19 PM (2 years, 2 months ago) |
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What did you inoculate with, and when?
Try this agar tek
-------------------- Two eyes to look, One eye to see. Prying open my third eye
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Smartattack
C'mon man
Registered: 12/21/18
Posts: 3,778
Loc: A thought
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE] 1
#27596629 - 12/27/21 06:20 PM (2 years, 2 months ago) |
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What are you trying to grow on it? Do you know that you are putting viable things on it? Even really low nutrients will get things going.
Edit. Mystic beat me basically
-------------------- * Smarts videos * Planet of the APES I'm a fungal white supremacist.
Edited by Smartattack (12/27/21 06:20 PM)
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O0ZE
Ascended Drone for the hive
Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 7 months
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Re: Screwed up Agar. Nothing is growing on it. [Re: Smartattack]
#27596651 - 12/27/21 06:37 PM (2 years, 2 months ago) |
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Mystic and Smartattack,
that's kinda why I don't get it, but this is only my 3rd try after 4 years off from the hobby and 2.5 successful big grows. The spores are at least 4 years old. I even pulled a dish from my SAB and got some gunk on it and NOTHIN. it should be crawling with bacteria right now, but it's not. The Agar is F37king useless. how can that be? the water was a sweet potato and oat base boiled for hours. I inoculated with Golden teachers using a print and sterile inoculation loops. prints came from the same friend that helped me with my first two grows, so I don't think it's the prints at all.
-------------------- Not everything that counts is countable and not everything that is countable counts.
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MysticMycologist
Dirt Sherpa
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE]
#27596664 - 12/27/21 06:45 PM (2 years, 2 months ago) |
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How many days ago did you put spores to agar. How long ago did you intentionally contam that test plate?
Edit, sorry I see you used a print.
-------------------- Two eyes to look, One eye to see. Prying open my third eye
Edited by MysticMycologist (12/27/21 06:46 PM)
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Smartattack
C'mon man
Registered: 12/21/18
Posts: 3,778
Loc: A thought
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Re: Screwed up Agar. Nothing is growing on it. [Re: MysticMycologist] 1
#27596675 - 12/27/21 06:54 PM (2 years, 2 months ago) |
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We'll be pissed when you say it's only been 5 days lol.
-------------------- * Smarts videos * Planet of the APES I'm a fungal white supremacist.
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O0ZE
Ascended Drone for the hive
Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 7 months
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Mystic, (thanks for your help)
I inoculated on the 21st and I intentionally contaminated a dish yesterday. I don't remember shit taking this long, but I have a terrible memory.
Edited by O0ZE (12/27/21 06:58 PM)
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MysticMycologist
Dirt Sherpa
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE]
#27596685 - 12/27/21 06:58 PM (2 years, 2 months ago) |
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Dude your agar is fine. Give it a couple weeks.
Smart attack called it.
-------------------- Two eyes to look, One eye to see. Prying open my third eye
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O0ZE
Ascended Drone for the hive
Registered: 02/28/18
Posts: 321
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Last seen: 1 year, 7 months
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oh... ok well that's good news. glad I'm still keeping things clean. I really don't remember things taking this long, but i know the whole process takes time. I'm in no rush.
should i increase nutrients on the next sleeve of petris?
-------------------- Not everything that counts is countable and not everything that is countable counts.
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Ashtray161
SettledNomad
Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Quote:
MysticMycologist said: Dude your agar is fine. Give it a couple weeks.
Smart attack called it.
Give it at least 14 days
-------------------- (You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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O0ZE
Ascended Drone for the hive
Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 7 months
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Re: Screwed up Agar. Nothing is growing on it. [Re: Ashtray161] 1
#27596715 - 12/27/21 07:20 PM (2 years, 2 months ago) |
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feels good to be back here. I don't recognize anyone yet.
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MysticMycologist
Dirt Sherpa
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE] 1
#27596748 - 12/27/21 07:37 PM (2 years, 2 months ago) |
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I use dilute grain soak water, but you can tweak your nutrients if you want. Cubensis is pretty forgiving.
-------------------- Two eyes to look, One eye to see. Prying open my third eye
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Smartattack
C'mon man
Registered: 12/21/18
Posts: 3,778
Loc: A thought
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Re: Screwed up Agar. Nothing is growing on it. [Re: MysticMycologist] 1
#27596802 - 12/27/21 09:06 PM (2 years, 2 months ago) |
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If anything your agar is going to be overboard if you boiled sweet potatoes and oats in it lol. Ive made a liter of agar that worked by just throwing in a random pinch of MEA powder.
-------------------- * Smarts videos * Planet of the APES I'm a fungal white supremacist.
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Kizzle
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE] 1
#27596853 - 12/27/21 11:06 PM (2 years, 2 months ago) |
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Quote:
O0ZE said: oh... ok well that's good news. glad I'm still keeping things clean. I really don't remember things taking this long, but i know the whole process takes time. I'm in no rush.
should i increase nutrients on the next sleeve of petris?
It will always seem like it's taking too long. You get used to watching colonies growing where there are noticeable changes every day and then you make some spore plates and there's nothing growing every day.
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bigfootscreepyuncl
Stranger
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Re: Screwed up Agar. Nothing is growing on it. [Re: Kizzle] 1
#27598022 - 12/28/21 10:11 PM (2 years, 2 months ago) |
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I usually get concerned when I get growth on my plates within 3-5 days
-------------------- I 5318008 NOT a virgin!
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Ashtray161
SettledNomad
Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Quote:
bigfootscreepyuncl said: I usually get concerned when I get growth on my plates within 3-5 days
-------------------- (You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Ankhseramun
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Re: Screwed up Agar. Nothing is growing on it. [Re: O0ZE]
#27598186 - 12/29/21 02:42 AM (2 years, 2 months ago) |
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The spores from my "fresh" Spore Syringe (no idea how long it was laying around in a warehouse) took around 1,5 weeks to just germinate on agar in ideal conditions, then about 2-3 weeks for good colonization to be ready for transfers. 4 year old spores will probably take more time, give it about 2 weeks, maybe 3, if there's nothing growing after this time, I'd say your spores are dead.
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Ashtray161
SettledNomad
Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Screwed up Agar. Nothing is growing on it. [Re: Ankhseramun]
#27598869 - 12/29/21 04:42 PM (2 years, 2 months ago) |
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Quote:
Ankhseramun said: The spores from my "fresh" Spore Syringe (no idea how long it was laying around in a warehouse) took around 1,5 weeks to just germinate on agar in ideal conditions, then about 2-3 weeks for good colonization to be ready for transfers. 4 year old spores will probably take more time, give it about 2 weeks, maybe 3, if there's nothing growing after this time, I'd say your spores are dead.
(edit. Whoops I misread)
-------------------- (You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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smalltalk_canceled
Babnik
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Re: Screwed up Agar. Nothing is growing on it. [Re: Ashtray161]
#27598922 - 12/29/21 05:15 PM (2 years, 2 months ago) |
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Some prints are crap, cheers for redundancy
-------------------- Willpower is the one true virtue
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O0ZE
Ascended Drone for the hive
Registered: 02/28/18
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Last seen: 1 year, 7 months
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Eureka! the spores began to grow mycelia! no tendrils though. I let them sit for awhile because what was growing was fuzzy. no change in color. they are still white. it's been about three weeks total. two to start and one of mycelial growth I think. posting pictures of the new life I've brought into this crazy world. the pictures are horrible by the way.
I made another sleeve of agar plates-20. this time I added dark malt extract and yeast. I have a question. why does Cubensis eat aluminum? I read on here once that it metabolizes aluminum. I want to observe this myself. any harm or foul in that?
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