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Offlinemrmycel
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Registered: 12/11/21
Posts: 41
Last seen: 1 year, 5 months
Fluffy layer on top of mycelium on agar, contam or no? (PICS)
    #27596618 - 12/27/21 06:12 PM (2 years, 1 month ago)

Did some transfers recently from these germ plates (cubensis) in this post

Seems to grow nicely, but the surface consists of a fluffy layer of something. Could it be anything other than mycelium? Present on ALL my 8-9 transfer plates to a high degree. The pictures show 5 days worth of growth.

Plate 1 - pretty fluffy:




Plate 2 - mega fluffy (different angles make a big difference)






Cheers!


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Offlinemrmycel
Stranger
Registered: 12/11/21
Posts: 41
Last seen: 1 year, 5 months
Re: Fluffy layer on top of mycelium on agar, contam or no? (PICS) [Re: Notarealguy]
    #27596987 - 12/28/21 06:24 AM (2 years, 30 days ago)

Those two transfer were the most successful ones. I'd appreciate it if someone could look at these that to me look less successful.

What I'm most curious about is the glassy looking edge on these photos. In the OP, such a glassy ring is not visible.

The reason it scares me is because it looks a lot like what I get on spore germ plates right before the mycelium growth stops. So I'm guessing it is bacteria?

This is my spore plate I didn't use that's like 3 weeks old now. Just made new germ plates though and they develop the same film within days.



It not as visible on a photo but you can clearly see the edge of the reflective film that has grown out, stopping the mycelium from expanding.

I wonder if this type of film is the same as we can seen on my other plates (also, what is it? Bacteria?):

Plate 3:

Plate 4:

Plate 5:


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