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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns] * 1
    #27710484 - 03/27/22 12:21 PM (2 years, 1 month ago)

Update: 3/27/22 original microwave jar

This was the original 3 minute microwave jar that I let sit for a month to see if any contamination would grow with it being un inoculated.

Nothing ever grew so I wanted to then put good mycelium on it to test that the grain was still viable and could host life on it so I transferred a wedge of summer white oyster in to it

The results speak for themselves,



--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offline7SunsS
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Re: Successful sterilization of oats in just 30 minutes [Re: 7Suns]
    #27711558 - 03/28/22 01:22 PM (2 years, 1 month ago)

That was a while ago, I done a lot more since then, just finished a test with 35 jars


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns] * 1
    #27792885 - 05/25/22 08:18 PM (1 year, 11 months ago)

Quote:

7Suns said:
So you guys remember the original jars that contaminated at the 2 week mark right at the top of the jar, probably because of the micropore tape?

Here’s an update of one of them.......



I’m actually very suspicious of it, I think it might be good fungi mycelium some how, and that made me think, I grow a lot of oyster in that room, what are the chances the spore load is so heavy some oyster spores could have found their way into the jar through the micropore??

I’m thinking I will spawn them to coconut coir for shits and giggles to see if it will produce a fruit body so I can attempt to identify it, it could be
schizophyllum commune??? They have been known to be a contaminate fungi





Funniest shit ever just wanted to give y’all an update
The 2 jars that originally contaminated we’re actually contaminated by the heavy spore load in the same room I grow in lmao I’ve never heard of or Sean this but apparently blue oyster spores are able to get through micro pore tape , to my surprise I went to clean out the lab and forgot about those contaminated jars and sure as shit it was pinning in vitro




--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns] * 1
    #27792888 - 05/25/22 08:19 PM (1 year, 11 months ago)

Also kept the 3 minute test microwave grain on agar around and looked at it yesterday, it’s still clean lmao 😂 didn’t take pics but I still can if y’all want


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offlinedyel
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
    #27793338 - 05/26/22 06:52 AM (1 year, 11 months ago)

cool, you now have fresh genetics without needing to go to agar

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: dyel]
    #27793395 - 05/26/22 08:02 AM (1 year, 11 months ago)

Quote:

dyel said:
cool, you now have fresh genetics without needing to go to agar




Didn’t think about that lol, I’ve been running the genetics into to ground with grain to grain lmao


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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OfflineDERRAYLD
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
    #27793482 - 05/26/22 09:26 AM (1 year, 11 months ago)

Oyster spore load is very heavy as you're aware, they definitely get through mp tape.
I always gre my oysters in their own filtered  tunnel.



Glad you've found success though.

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Offlinedyel
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Re: Possible success sterilizing oats in just 30 minutes? [Re: DERRAYLD]
    #27793600 - 05/26/22 11:18 AM (1 year, 11 months ago)

not trying to dirail the thread but since there is a lot of slack time due the subject in hand I'm gonna go ahead and ask
do you guys go back to spores after some time or do you just buy new LC from someone else?
I'm asking because I'm growing supermarket clones and I'm not sure how long I can keep pushing it
and if you do what is the best approach for isolation, i see a lot talking about cubes should I just select the fastest growing sectors?
thanks in advance

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: dyel]
    #27794326 - 05/26/22 08:21 PM (1 year, 11 months ago)

I haven’t had to restart mine yet but what I like to do is make 10 slants when I first get the mother culture and then put them in the fridge so when you start to see genetic degradation, just start back at square one with a slant, depending on how much you produce that should last a few years before you would need to go back to spores


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: DERRAYLD]
    #27794333 - 05/26/22 08:23 PM (1 year, 11 months ago)

Quote:

DERRAYLD said:
Oyster spore load is very heavy as you're aware, they definitely get through mp tape.
I always gre my oysters in their own filtered  tunnel.



Glad you've found success though.




Yea it blew my mind that they where able to get through the mp tape, I guess it’s a numbers game, if the air is heavily saturated it just a matter of time before the dam breaks metaphorically speaking


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
    #27794355 - 05/26/22 08:48 PM (1 year, 11 months ago)

Oh and I think for my next experiments I’m going try simmering grain with lime + oxyclean since it’s essentially concentrated hydrogen peroxide, and yes I’ll be using the “free and clear” version since it doesn’t contain any dyes or fragrances and the best part is when its heated it breaks down into oxygen and water so it shouldn’t leave behind anything that will hinder the good mycelium, and yes I’ve done research into concentrated peroxide being explosive, but this is still diluted enough that is not an issue, people actually boil deer skulls in oxyclean on the stove and they don’t blow up lmao


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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InvisibleCreonAntigone
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Registered: 05/30/21
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
    #27795547 - 05/27/22 06:49 PM (1 year, 11 months ago)

How about tannins? I once tried infusing grains with triphala tea. Triphala is an indian medicine made from three very tannic fruits. I made super strong tea from it, which would be bitter if anyone drank it, and seeped it into the grains. Nothing at all germinated in those jars, even though I added spores. Then I tried to open and close them a few times. And nothing still germinated in them. With a certain tannin content, nothing could grow even with all that available nutrition.

If one could infuse the right percent of tannins, one might be able to inhibit growth and not stop it entirely.

There are much cheaper ways to get tannins than special indian medicine - leeching acorns and sawdust would work.

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Offline7SunsS
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Re: Possible success sterilizing oats in just 30 minutes? [Re: CreonAntigone]
    #27796098 - 05/28/22 06:21 AM (1 year, 11 months ago)

I think wood loving strains would probably benefit the most from using tannins since they are probably already somewhat tolerant


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

Extras: Filter Print Post Top
Offline7SunsS
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I'm a teapot User Gallery


Registered: 05/11/14
Posts: 765
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
    #28769194 - 05/10/24 12:03 PM (8 days, 9 hours ago)

A lot has changed since i started experimenting with lime but i have a few updates i want to share

I was able to successfully sterilize oats using atmospheric steam and lime for 24 hours

I’m planning to conduct more experiments with shorter time and i hope to be able to get it down to just an hour or two using atmospheric steam and whole oats


Update: https://www.shroomery.org/forums/showflat.php/Number/28777482


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

Edited by 7Suns (05/18/24 12:27 AM)

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   OlympusMyco.com Olympus Myco Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Grow Bags   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag


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