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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,779
Loc: A thought
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27691665 - 03/11/22 08:26 PM (2 years, 2 months ago) |
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Quote:
7Suns said: everyone here can be a stickler for even the slightest variable I should have known better
Well, welcome to science man.
Good on you for explaining I guess. That said, this thread isn't holding my interest so much at this point. Its nothing personal and I'm not going to rag on you unnecessarily, but its feeling like a bad 3rd date with a girl where the first two didn't go great either but you hung in there for potential. Ill text you in a month or so and see how you are doing.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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milkboy
Child



Registered: 05/01/21
Posts: 2,390
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Re: Possible success sterilizing oats in just 30 minutes? [Re: Smartattack]
#27691672 - 03/11/22 08:31 PM (2 years, 2 months ago) |
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-------------------- Gummies by milkboy Milkboys Public Display of Affection When the student is ready, the teacher appears
  
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: Smartattack]
#27691702 - 03/11/22 09:02 PM (2 years, 2 months ago) |
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I’m fucking crying laughing so hard
thats probably the funniest shit I’ve read on here but I appreciate the honesty hopefully I’ll have something more interesting for you next time lol, I just figured since you were so adamant about the internal temperatures of the jars I’d share it with you,
I’m going to open the rest of the jars and check those indicator strips tomorrow as well if you pop back in either way it’s always amusing to see what kind of criticism you have to dish out lol
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Speeker

Registered: 02/11/04
Posts: 894
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27692130 - 03/12/22 07:24 AM (2 years, 2 months ago) |
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Those strips might give you a general idea how hot it's in various parts of your PC. I.e putting bags too close together blocks heat or steam from getting into them.
Also the air is a good a insulator, within the jars too. The heat might go nicely go along the side of those bottles inside, but might stop into air-pockets between kernels too close together.
So, maybe this how-hot thing is a bit irrelevant? Maybe PC isn't needed even.. Maybe just a quick pasteurisation would be enough? IDK..
What I would like to see a heatless method for cultivation. Starting from the begining, i.e. spores. (Maybe using H2O2 could just do it, but it might take takes a while to start spores with it..) (edit: another way of starting from a print might be that one puts a spore print between cardboards. Worked once nicely in a hot balcony for me. Found a bunch of rotten fungi after the holidays..)
Edited by Speeker (03/12/22 08:35 AM)
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7Suns
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Re: Possible success sterilizing oats in just 30 minutes? [Re: Speeker]
#27692317 - 03/12/22 10:51 AM (2 years, 2 months ago) |
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Quote:
Speeker said: Those strips might give you a general idea how hot it's in various parts of your PC. I.e putting bags too close together blocks heat or steam from getting into them.
Also the air is a good a insulator, within the jars too. The heat might go nicely go along the side of those bottles inside, but might stop into air-pockets between kernels too close together.
So, maybe this how-hot thing is a bit irrelevant? Maybe PC isn't needed even.. Maybe just a quick pasteurisation would be enough? IDK..
What I would like to see a heatless method for cultivation. Starting from the begining, i.e. spores. (Maybe using H2O2 could just do it, but it might take takes a while to start spores with it..) (edit: another way of starting from a print might be that one puts a spore print between cardboards. Worked once nicely in a hot balcony for me. Found a bunch of rotten fungi after the holidays..)
Yes I’m going to also test steam sterilization at atmospheric pressure (212f)
And unfortunately in gourmet cultivation (which is all I do these days) we don’t really start from spores, it’s always cultures or g2g, and I’m not sure what you’re train of thought is about peroxide and spore germination but I have a Tek in my bio for antibiotic agar from scratch if that’s relevant
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Speeker

Registered: 02/11/04
Posts: 894
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27692630 - 03/12/22 03:19 PM (2 years, 2 months ago) |
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Quote:
7Suns said: Yes I’m going to also test steam sterilization at atmospheric pressure (212f)
And unfortunately in gourmet cultivation (which is all I do these days) we don’t really start from spores, it’s always cultures or g2g, and I’m not sure what you’re train of thought is about peroxide and spore germination but I have a Tek in my bio for antibiotic agar from scratch if that’s relevant
Once one has learned the labs it's relatively easy and already paid at that point. The idea was that let's make it easy for anyone without a "lab"***. Even for these blue fungi enthusiasts here. And maybe with better results than just pushing pieces of fruitbodies into logs, which can actually work quite well - sometimes... I have done it with various spp. At moment I have K. mutabilis growing in LC with some H2O2 in it, from spores, no labs involved. Spores were taken from mushroom logs that were done like mentioned.. Then again it might be a mold naturally, but looking good so far growing on the surface. But if it is a mold, at least I'ven't put much work on it...
***edit: have you ever made tempeh? That easy... (typos and more of them)
Edited by Speeker (03/12/22 03:32 PM)
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: Speeker]
#27692659 - 03/12/22 03:38 PM (2 years, 2 months ago) |
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Quote:
Speeker said:
Quote:
7Suns said: Yes I’m going to also test steam sterilization at atmospheric pressure (212f)
And unfortunately in gourmet cultivation (which is all I do these days) we don’t really start from spores, it’s always cultures or g2g, and I’m not sure what you’re train of thought is about peroxide and spore germination but I have a Tek in my bio for antibiotic agar from scratch if that’s relevant
Once one has learned the labs it's relatively easy and already paid at that point. The idea was that let's make it easy for anyone without a "lab"***. Even for these blue fungi enthusiasts here. And maybe with better results than just pushing pieces of fruitbodies into logs, which can actually work quite well - sometimes... I have done it with various spp. At moment I have K. mutabilis growing in LC with some H2O2 in it, from spores, no labs involved. Spores were taken from mushroom logs that were done like mentioned.. Then again it might be a mold naturally, but looking good so far growing on the surface. But if it is a mold, at least I'ven't put much work on it...
***edit: have you ever made tempeh? That easy... (typos and more of them)
Not sure how any of that was relevant but ok
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Speeker

Registered: 02/11/04
Posts: 894
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27692672 - 03/12/22 03:47 PM (2 years, 2 months ago) |
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Quote:
7Suns said: Not sure how any of that was relevant but ok 
Yeah.. maybe not relevant, but there is connection to this shit of yours. Maybe this turns into something useful after all.. let's hope so..
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: Speeker]
#27692686 - 03/12/22 03:53 PM (2 years, 2 months ago) |
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Lol yea it’s useful and lots of connections
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Speeker

Registered: 02/11/04
Posts: 894
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27692727 - 03/12/22 04:19 PM (2 years, 2 months ago) |
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Quote:
7Suns said: Lol yea it’s useful and lots of connections
Oh yeah, prove it! LOL.
Really, I think you should just say what you do or did and that is it. Let them prove otherwise if they want..
There have been many many methods before and used and made better and such.. not always this prove it shit. Maybe it is a bit because how you brought out this idea of yours.. IDK..  Also, if you/we can do the same with just by boiling that PC heat stuff is irrelevant.
edit: grammar
Edited by Speeker (03/12/22 05:16 PM)
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7Suns
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Re: Possible success sterilizing oats in just 30 minutes? [Re: Speeker]
#27692917 - 03/12/22 06:22 PM (2 years, 2 months ago) |
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I agree and appreciate the encouragement
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27694448 - 03/13/22 11:18 PM (2 years, 2 months ago) |
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As much as I hate to be wrong, I’m a man of my word and this is all in the name of science so it’s my moral duty to disclose all findings unbiased
I opened the other 6 jars that were prepared via lime hydration and to my dismay the steam indicator strips inside the grain failed suggesting different preparation make a difference and that traditional hydration prior to pressure cooking fails to reach 250f in 15 minutes after the weight starts rocking
Although I also need to do research if ph has any effect on the indicator tape
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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SingularFusion


Registered: 10/31/18
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27696048 - 03/15/22 08:40 AM (2 years, 2 months ago) |
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appreciate your honesty man
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: SingularFusion]
#27696110 - 03/15/22 09:41 AM (2 years, 2 months ago) |
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I just resterilized the same grain jars but this time I replaced the tape on the inside and outside and put the number 2 in red sharpie on it for my second test batch
This time they were sterilized for 30 minutes after the rocker started so hopefully the center vials reached 250f if not I will repeat this test in 15 minute increments until successful color change has occurred on the center vials
The importance of this test is to once and for all establish at what time interval the center of each jar reaches critical temperature
Also my other set of time/temp indicators arrived so we can more accurately determine when 250f is not only reached but also maintained for 15 minutes to complete the sterilization process
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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7Suns
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27696118 - 03/15/22 09:47 AM (2 years, 2 months ago) |
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Also just wanted to mention I have a new chemical disinfectant that breaks down clean and only leaves behind water as a byproduct so I’m going to start adding it to the lime grain either in the final rinse or together in the the hydration step to see if I can eliminate heat from the equation altogether
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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7Suns
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27696578 - 03/15/22 04:36 PM (2 years, 2 months ago) |
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Just checked the results for the steam indicator tape on the second batch
The first jar I pulled in open air confirmed 250f internal temperature so, I’m going to open the other 6 in the flowhood to confirm consistency among the other test jars
Next I’m going to do a 30 minute pc cycle with my new “yipak” class 4 indicator strips that tell time and temperature to determine when the internal temperature of the grain has been held for a minimum of 15 minutes to establish proper sterilization requirements
If further testing is required after batch 3 is established I might shell out the funds to do a biological indicator test which is the highest authority when it comes to steam sterilization testing
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27705437 - 03/23/22 06:03 AM (2 years, 1 month ago) |
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: Rotnpins]
#27705574 - 03/23/22 09:27 AM (2 years, 1 month ago) |
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Will update soon, been a bit busy
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns] 1
#27710416 - 03/27/22 11:17 AM (2 years, 1 month ago) |
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UPDATE: 3/27/2022
This was a batch of 36 quart jars with different grains and different strains
My conclusion is that the strain and grain doesn’t make much difference with the the exception being that lions mane seems to be negatively affected by the lime otherwise everything else preformed similarly
There was however a hiccup in my research, we had 15 out of 36 jars fail and it appears to be the result of obligate anaerobes because the top 3/4 of the jar colonized beautifully and the bottom 1/4 (where there’s low oxygen) seems to have stalled completely even after shaking to redistribute, the same thing happens again,
I’ve already devised a solution for this by either bumping the time back up to 30 minutes (now that we know the center of the grain reaches 250 in 30 minutes) and running another large batch or by adding another chemical sanitizer to the process in another trial run to verify if it can eliminate the obligate anaerobes (I have a good candidate in mind with all the right characteristics)
I’m under the impression the anaerobe responsible is the clostridium botulinum bacterium which is known for causing botulism in improperly canned food items, which makes sense because it’s in “canned” grain With low oxygen so it fits the bill as an obligate anaerobe, it’s also gram positive making lime hydration less effective against it, another tell tell sign is that it’s one of the few gram positive obligate anaerobes that produce endospores, making it the most logical culprit, knowing this will help me strategize a way to combat it.
Here are the photos of the 36 jars, they were separated into 2 groups the good and the bad
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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7Suns
Researcher



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Re: Possible success sterilizing oats in just 30 minutes? [Re: 7Suns]
#27710472 - 03/27/22 12:14 PM (2 years, 1 month ago) |
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Update: 3/27/22 steam indicator tape
So I let those test jars that were ran for 30 minutes with the indicator tape sit for 2 weeks now, there’s no contamination visible or when I smelled the grain, I decided not to open in the flowhood, but yea every jar reach 250f internally at 30 minutes from the time the weight starts rocking so I believe 30 minutes pc + lime is the appropriate method so far which still beats 90 minutes
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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