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mrmycel
Stranger
Registered: 12/11/21
Posts: 41
Last seen: 1 year, 5 months
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Input on mycelium on my first agar
#27587345 - 12/19/21 02:15 PM (2 years, 1 month ago) |
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Hi, cultivating shrooms the first time. Started with agar right away. Can anyone spot contamination? Getting ready to do transfers!
Scraped spores onto four plates. Black specks are spores.
Plate 1 - has the only clean looking mycelium, i.e. as I expected it to look. But I think the rest is mycelium as well, what do you think?:

Plate 2 - less growth. No nice looking mycelium like plate 1. If plate 1 is mycelium only, then this is too I suppose

Plate 3 - same as plate 2

Plate 4 - bacterial growth from what I've gathered. Thin film all over the plate and no other growth. Appeared in just a couple of days.

Considering how plate 4 looks, I'm a bit concerned about the other plates as well. They all have a border that looks similar, so I'm wondering if it could be bacterial growth as well under the mycelium. Difficult to get a good photo, but here's an attempt.
Edited by mrmycel (12/19/21 02:16 PM)
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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,502
Last seen: 8 months, 28 days
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Re: Input on mycelium on my first agar [Re: mrmycel]
#27587386 - 12/19/21 02:50 PM (2 years, 1 month ago) |
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plate 1 3-5 o'clock and do it fast imho the rest are a mess, and even plate 1 might be a stretch but is the best you got.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,774
Loc: A thought
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Re: Input on mycelium on my first agar [Re: kanemush]
#27587428 - 12/19/21 03:26 PM (2 years, 1 month ago) |
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You have stuff to work with a plenty. You definitely circled the best growth and you should get transfers started now. Germ plates are a mine field so it's best to get the goods off as soon as you can.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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mrmycel
Stranger
Registered: 12/11/21
Posts: 41
Last seen: 1 year, 5 months
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Re: Input on mycelium on my first agar [Re: Smartattack]
#27590714 - 12/22/21 01:11 PM (2 years, 1 month ago) |
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Quote:
kanemush said: plate 1 3-5 o'clock and do it fast imho the rest are a mess, and even plate 1 might be a stretch but is the best you got.
Quote:
Smartattack said: You have stuff to work with a plenty. You definitely circled the best growth and you should get transfers started now. Germ plates are a mine field so it's best to get the goods off as soon as you can.
Thanks guys. I did 10 transfers and some new germ plates with a better PC just in case it all goes to hell. Some plates ought to succeed eventually.
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