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Offlinemrmycel
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Registered: 12/11/21
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Input on mycelium on my first agar
    #27587345 - 12/19/21 02:15 PM (2 years, 1 month ago)

Hi, cultivating shrooms the first time. Started with agar right away. Can anyone spot contamination? Getting ready to do transfers!

Scraped spores onto four plates. Black specks are spores.

Plate 1 - has the only clean looking mycelium, i.e. as I expected it to look. But I think the rest is mycelium as well, what do you think?:


Plate 2 - less growth. No nice looking mycelium like plate 1. If plate 1 is mycelium only, then this is too I suppose


Plate 3 - same as plate 2


Plate 4 - bacterial growth from what I've gathered. Thin film all over the plate and no other growth. Appeared in just a couple of days.


Considering how plate 4 looks, I'm a bit concerned about the other plates as well. They all have a border that looks similar, so I'm wondering if it could be bacterial growth as well under the mycelium. Difficult to get a good photo, but here's an attempt.


Edited by mrmycel (12/19/21 02:16 PM)


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Offlinekanemush
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Re: Input on mycelium on my first agar [Re: mrmycel]
    #27587386 - 12/19/21 02:50 PM (2 years, 1 month ago)

plate 1 3-5 o'clock and do it fast imho the rest are a mess, and even plate 1 might be a stretch but is the best you got.


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InvisibleSmartattack
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Re: Input on mycelium on my first agar [Re: kanemush]
    #27587428 - 12/19/21 03:26 PM (2 years, 1 month ago)

You have stuff to work with a plenty. You definitely circled the best growth and you should get transfers started now. Germ plates are a mine field so it's best to get the goods off as soon as you can.


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Offlinemrmycel
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Registered: 12/11/21
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Last seen: 1 year, 5 months
Re: Input on mycelium on my first agar [Re: Smartattack]
    #27590714 - 12/22/21 01:11 PM (2 years, 1 month ago)

Quote:

kanemush said:
plate 1 3-5 o'clock and do it fast imho the rest are a mess, and even plate 1 might be a stretch but is the best you got.




Quote:

Smartattack said:
You have stuff to work with a plenty. You definitely circled the best growth and you should get transfers started now. Germ plates are a mine field so it's best to get the goods off as soon as you can.




Thanks guys. I did 10 transfers and some new germ plates with a better PC just in case it all goes to hell. Some plates ought to succeed eventually.


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