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InvisibleaNewBeginning

Registered: 06/26/21
Posts: 708
Re: Post Your New Tools Of The Trade. [Re: TedsDead]
    #27565443 - 12/01/21 10:29 PM (2 years, 5 months ago)

Yeah copper was not a great idea, in hindsight.

The handle has held up well, and I'm standing by that :P

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OfflinexVadisx
MountainsDogsAndFungi
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Registered: 09/03/21
Posts: 1,321
Loc: Secret World HQ
Last seen: 10 hours, 56 minutes
Re: Post Your New Tools Of The Trade. [Re: Failboat] * 1
    #27565685 - 12/02/21 05:32 AM (2 years, 5 months ago)

Quote:

Quirkmeister92 said:
I like my scalpel and blades. Once my culture is clean I cut strips of agar into tiny squares and expand 1 into stacks. Takes a few minutes and they grow picturesque enough.

Yalls punch plates are pretty, but if it's destined to be smooshed perhaps more time on all of the other work will be more fruitful.

MacGyver would be proud I'm sure :wink:




I'm all for a solid MacGyver moment -- but am also a fan of a scalpel and a sharp blade! Tried and true.


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
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Invisiblemicelio
Song of Silence
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Registered: 04/22/14
Posts: 1,547
Re: Post Your New Tools Of The Trade. [Re: xVadisx]
    #27565927 - 12/02/21 09:28 AM (2 years, 5 months ago)

Quote:

xVadisx said:
Quote:

Quirkmeister92 said:
I like my scalpel and blades. Once my culture is clean I cut strips of agar into tiny squares and expand 1 into stacks. Takes a few minutes and they grow picturesque enough.

Yalls punch plates are pretty, but if it's destined to be smooshed perhaps more time on all of the other work will be more fruitful.

MacGyver would be proud I'm sure :wink:




I'm all for a solid MacGyver moment -- but am also a fan of a scalpel and a sharp blade! Tried and true.





I use my scalpel most of the time. But there are situation that the needle punch can extract clean culture out of a tight spot on a dirty dish..


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OfflineFailboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 25 days, 5 hours
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Re: Post Your New Tools Of The Trade. [Re: micelio]
    #27565954 - 12/02/21 09:43 AM (2 years, 5 months ago)

Fair nuff. Don't tell Josex but I've not tried a needle poke. I have several cases of petris on hand and I'm not afraid of a extra transfers. Plates are much cheaper than cocaine and nobody's ripping me off about it either:rofl:

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InvisibleDrboomer
The lord magnificent
I'm a teapot User Gallery

Registered: 09/22/19
Posts: 957
Re: Post Your New Tools Of The Trade. [Re: Failboat]
    #27566315 - 12/02/21 02:12 PM (2 years, 5 months ago)

I use my scalpel too mostly. I use the punched on the days my hand is fucked up and won't cooperate. I'm on that josex train, as soon as shits clean poke to lc. Or straight to li if I'm doing a small run.  I'm going to all bottles though pretty much. It's just easier for me. It's faster and I don't have to do much myco work.

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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
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Re: Post Your New Tools Of The Trade. [Re: Drboomer]
    #27566885 - 12/02/21 08:52 PM (2 years, 5 months ago)

#ScalpelGang


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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InvisibleRaven44
Entry not permitted to muggles
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Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
Re: Post Your New Tools Of The Trade. [Re: LotKid] * 2
    #27567595 - 12/03/21 12:24 PM (2 years, 5 months ago)

I’ve noticed one common flaw with agar punching in general

Every tool I’ve seen forces the user to have his hand directly above the sample. This is not ideal and can create problems

Ideally the punch tool would allow the users hands to be as far away from the sample as possible while retrieving and/or “punching” the agar disc out

Personally I’m not attracted to punch tools at all but for those lacking in precise hand movements I can see it may be handy. Shaky hand days can be a real bummer, sobriety seems to help a lot tho ime.

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OfflineAbabyphoenix
You
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Registered: 03/21/19
Posts: 1,015
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Re: Post Your New Tools Of The Trade. [Re: Raven44]
    #27567605 - 12/03/21 12:33 PM (2 years, 5 months ago)

I used to punch and ran into that same thought. To fix is i started picking my plates up in my left hand and tilting them so i could punch out the transfers at an angle without my hand going over them. Just a thought.


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:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Invisiblemilkboy
Child
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Registered: 05/01/21
Posts: 2,389
Re: Post Your New Tools Of The Trade. [Re: Ababyphoenix]
    #27567640 - 12/03/21 12:57 PM (2 years, 5 months ago)

Shit I even do that with my ketchup cups when I use a scalpel. Alot easier for me to see/cut when im holding it. Except I use my right hand to hold the cup cause im a lefty :grin:


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Gummies by milkboy
Milkboys Public Display of Affection

When the student is ready, the teacher appears


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OfflineAbabyphoenix
You
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Registered: 03/21/19
Posts: 1,015
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Last seen: 2 months, 25 days
Re: Post Your New Tools Of The Trade. [Re: milkboy]
    #27567687 - 12/03/21 01:35 PM (2 years, 5 months ago)



Another bag of poly and more pretty pwetty for my agar


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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OfflineHindsight
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Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 28 days
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Re: Post Your New Tools Of The Trade. [Re: Raven44]
    #27567953 - 12/03/21 05:20 PM (2 years, 5 months ago)

Quote:

Raven44 said:
I’ve noticed one common flaw with agar punching in general

Every tool I’ve seen forces the user to have his hand directly above the sample. This is not ideal and can create problems

Ideally the punch tool would allow the users hands to be as far away from the sample as possible while retrieving and/or “punching” the agar disc out

Personally I’m not attracted to punch tools at all but for those lacking in precise hand movements I can see it may be handy. Shaky hand days can be a real bummer, sobriety seems to help a lot tho ime.




I made a home-brew punch. It has a plunger in it in case the plug gets stuck inside the stainless tube but that only happens 1 out of 20 times. I hold the plate at a steep angle (nearly 90 degrees) and then punch. That way nothing is over the plate. Then I set the punch down and while keeping the plate at a steep angle, grab my knife and pull the plug out then put it onto the receiving plate.

The only reason I like using a punch is that it helps you see if the mycelium grows evenly and helps you see real sectors better.

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OfflineA.k.aM
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Registered: 10/27/19
Posts: 17,072
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Re: Post Your New Tools Of The Trade. [Re: Hindsight]
    #27568003 - 12/03/21 06:17 PM (2 years, 5 months ago)

Yeah I hold my plates sideways while cutting a lot too.


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LAGM2020

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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Post Your New Tools Of The Trade. [Re: Ababyphoenix] * 1
    #27568113 - 12/03/21 07:40 PM (2 years, 5 months ago)

Seems like extra work to me just to not use a scalpel. I also dont need to sterilze my scalpel before use. Iso the handle and fresh blades are sterile.

Call me lazy, i guess. :shrug:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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OfflineFailboat
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Re: Post Your New Tools Of The Trade. [Re: LotKid] * 2
    #27568116 - 12/03/21 07:42 PM (2 years, 5 months ago)

I torch the same ol crusty blade till it falls off.:smoker:

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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
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Re: Post Your New Tools Of The Trade. [Re: Failboat]
    #27568143 - 12/03/21 07:57 PM (2 years, 5 months ago)

I go thru more blades doing transfers than i do innoculations. But yes, torch for the win


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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OfflineAbabyphoenix
You
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Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 2 months, 25 days
Re: Post Your New Tools Of The Trade. [Re: Failboat]
    #27568151 - 12/03/21 08:03 PM (2 years, 5 months ago)

Quote:

Quirkmeister92 said:
I torch the same ol crusty blade till it falls off.:smoker:



Yep. Same lmfao. Not that my word means shit but. Torch ftw


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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OfflineSpaceBaby
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Registered: 11/01/20
Posts: 2,030
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Re: Post Your New Tools Of The Trade. [Re: Ababyphoenix]
    #27568180 - 12/03/21 08:21 PM (2 years, 5 months ago)

bactizapper for me


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

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OfflineHindsight
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Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 28 days
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Re: Post Your New Tools Of The Trade. [Re: SpaceBaby]
    #27568183 - 12/03/21 08:27 PM (2 years, 5 months ago)

Charcoal blade gang here too

As for a punch and the technique I mentioned above - it’s definitely not fast. If someone were doing 30+ transfers in a sitting I could see what a pain it would be but for a dozen or so it’s fine. To me, the growth pattern and consistency in comparison between plates that using plugs provides is worth it.

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Offline0ptiquest
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Registered: 07/05/20
Posts: 509
Last seen: 2 months, 18 days
Re: Post Your New Tools Of The Trade. [Re: SpaceBaby]
    #27568218 - 12/03/21 08:49 PM (2 years, 5 months ago)

Quote:

SpaceBaby said:
bactizapper for me




:whathesaid: never going back to torch

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Offlinedeadmandave
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Registered: 02/16/10
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Re: Post Your New Tools Of The Trade. [Re: 0ptiquest]
    #27568251 - 12/03/21 09:19 PM (2 years, 5 months ago)

Alcohol lamp and a fresh blade every other year works for me.

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