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OfflineLostkeys
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Haywire]
    #27393661 - 07/19/21 12:00 PM (2 years, 9 months ago)

I really want to try this Tek, but I'm having a terrible time sourcing these containers locally. I have quart jars and plastic lids, but I imagine these make it difficult to harvest.

Edit: I managed to find off brand PP5 containers of this exact sort at Target.

Edited by Lostkeys (07/19/21 01:32 PM)

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OfflineMakusan
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Lostkeys]
    #27416993 - 08/06/21 02:41 PM (2 years, 9 months ago)

Ok this GS Tek, calls for "Lawn" grass seed...NOT Rye grass seed. It appears that the grass seed(Fescue)is the one. I'm still not sure (here a few others)  If someone could please clarify which one?!
(https://www.chegg.com/flashcards/cool-season-grass-seed-list-51070b52-ecda-4c8f-8c5b-b65341cb76be/deck). -namaskaram

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Offlineblackout
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Makusan] * 1
    #27418239 - 08/07/21 01:20 PM (2 years, 9 months ago)

Quote:

Makusan said:
Ok this GS Tek, calls for "Lawn" grass seed...NOT Rye grass seed.



can you quote the bits that gave you that impression?

It is using RGS, commonly used on lawns.

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OfflineMakusan
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: blackout]
    #27420395 - 08/08/21 09:52 PM (2 years, 9 months ago)

"On the right is rye grain. Although it works quite nicely with this technique, it is not the grain here referenced.
On the left is our grass seed. Around these boards it's been known as "rye" grass seed, and much of the world's lawn seed is also called "rye." There are ~9000 identified species of grasses on this planet at the moment.
Regardless of what nickname it's been given, we're talking about lawn grass here. This definitely slims that category down a lot but it is not so slimmed as to include only one variety of a grass species. If it looks like the seed on the left, it's the stuff."

See pic. I would like to know which Grass Seed specifically is on the Left side? It might be a "Tall Fescue Grass Seed"?!


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OfflineGrounder
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Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Violet]
    #27563022 - 11/30/21 11:30 AM (2 years, 5 months ago)

1 thing that I may have missed out on here.

when colonizing can the lids be on tight or would you unscrew them a tad until done colonizing and then unscrew loose as mentioned in the OP?

I just can't remember if the myc needs FAE during colonization?

Thanks.

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Invisiblettching8475
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Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Grounder]
    #27563284 - 11/30/21 02:37 PM (2 years, 5 months ago)

Keep the lids on tight during colonization and then loosen for fruiting.


--------------------
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OfflinebrainAcid
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Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: ttching8475]
    #27577512 - 12/11/21 03:15 PM (2 years, 5 months ago)

Violet thank you very much. This is so enjoyable. I have this love for your work. I cant wait to have some nice fruits to upload some pics. I read all your stuff with great joy. Will soon have PC and grass seeds to do some nice GTs and also will try the poms tek. I'm going to experiment with off brand Orbeez and show some results. I want to soak them in the grain water. I wonder....

Thank you for this tek. It's the method I use and I'm very happy with all the work you have done.

Gracias.

Currently using Minute/Uncle Ben's brown rice cups/bags in front of a homemade flowhood made with a mattress pump into knock offs lid lock plastics I found at dollar tree. These china-made cheap not PP5. I have those on the way with the PC.

https://imgur.com/a/ZZflSb4
https://imgur.com/a/K7mmtWG

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OfflinebrainAcid
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: brainAcid]
    #27577580 - 12/11/21 03:55 PM (2 years, 5 months ago)

Quote:


Violet said:
The remaining concern is making sure the pinning surface itself doesn't dry out which would kill any pins before the have a chance to grow.





Come in Orbeez.

Quote:

Google said:

The scientific explanation behind this magic is that Orbeez are super absorbent polymer beads made of acrylic acid, sodium hydroxide and water, to which color pigmentation is added to give more variety of nuances. It is the water molecules that make Orbeez grow.






I have seen the mycellium colonize with no issues. There is a Reddit as well that people having success as a "neglect tek". What if we made a layer of grain water absorbed beads and a layer of clean water beads and PC them and try a new pom tek. I love this hobby!

Thanks Violet for all this!

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OfflinebrainAcid
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: brainAcid]
    #27577599 - 12/11/21 03:59 PM (2 years, 5 months ago)


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OfflinebrainAcid
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: brainAcid]
    #27577660 - 12/11/21 04:40 PM (2 years, 5 months ago)

Quote:

Amazon says:

Details
Brand EBOOT
Water crystal beads: designed to absorb and retain water, with good water absorption, they will gradually grow up in water; Note: keep them out of direct sunlight
Size: the beads are about 1 mm in diameter; They will grow up to 9 - 11 mm after absorbed enough water
Expandable material: water pearl beads are made from safe and tested polymer that make the beads expand when soaked in water
Wide usage: act as substitutes for water in your vases or just as a decorative display, also good for weddings, shower parties decorations
Package includes: approx. 10000 pieces of water crystal beads in one bag; Weight: approx. 50 g per bag;
Available colors: clear, black




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Offlinegun fuy
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: attfalt]
    #28010339 - 10/22/22 07:58 AM (1 year, 6 months ago)

Quote:

attfalt said:
I can't get these kinds of containers where I live (the ziplocs), how would the method differ if I were to use 1 liter glass jars instead? Would loosening the lid work for air exchange as well or would I have to use holes + polyfill? Does it make a difference if they're not wide-mouth?

As a first timer I'm pretty excited about finding this tek and I don't want to make any assumptions





I'm also a total noob and enjoying reading through some of Violets stuff. Interesting about the strain selection, sounds like a bit of fun.

I noticed that nobody answered to attfalts question quite a while back and I actually wanted to ask more or less the same thing.
Why is it that glass would be inferior for these TEKs?
Noticed a mention of using glass but with plastic lids instead of the usual metal lids.
What would be the problem/disadvantage with the metal lids?


Regarding the containers, I've seen some 1L plastic containers where I live from the brand TATAY. They're PP5 and on them it gives symbols saying it's freezer and microwave safe but max 100 degrees. Does this mean no good for the PC?


--------------------
"Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save." - RR

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Offlinebongoman
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: gun fuy]
    #28011859 - 10/23/22 02:25 AM (1 year, 6 months ago)

Violet certainly left a unique legacy here, her posts are pretty interesting even if she is seen as a controversial maverick. There's no doubting that there was a strong logical consistency to her approach borne from much experimentation. I still hope and wonder if she might make an appearance here again one day! Her threads are some of my favorite here on the shroomery.

I think Violet liked PP5 because it is easier to handle and manage than glass and also requires less sterilisation time.

There's no reason that I can see why her teks wouldn't work with glass. Mudafuka bottles for example, another in vitro method that is great, uses glass or PP5 interchangeably. You would need to increase PC time a bit though.

If the Tatay containers are actually pp5 then you are good. I'd maybe do a test run in your pressure cooker — add a cup of water to one and PC it for 60mins and see how it holds up. Plastic that isn't PC ready will at best distort in shape, or at worst, totally melt.

If you can get some air exchange with metal lids, then I can't see why they wouldn't work. Again, in the mudafuka bottles  thread, there are pics of glass jars with both plastic lids and modified metal lids.

I'd modify the lids by drilling holes and using with an SFD but that's just because that's how I do my lids for grain jars in general. Again, any mason jar lid tek should work here.

Edited by bongoman (10/23/22 02:26 AM)

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OfflineMoopers
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: bongoman]
    #28011927 - 10/23/22 04:07 AM (1 year, 6 months ago)

Glass isn't great because the mouth taper makes it harder to harvest and makes the fruits crowded if tall, and the metal canning lids might not allow enough FAE for fruiting. If you could find straight-sided 1L glass containers with screw on plastic lids or single-piece metal lids (not two-piece canning lids), it would work the same.

I did maybe 2 dozen of the 1L bottles when I first was growing cubes, and the tek works well enough, except that getting enough FAE without drying out the grains/casing can be a challenge after the first flush, depending on climate and air flow of the room. Fruits were leggy from needing more oxygen, or if I put the lids askew to increase FAE, they would get too dry.

It was also prone to side pins after the first flush when the sub (grains + casing) shrank, but that's often true of unlined monos, too. Bottom watering to solve the humidity/drying problem eventually would breed bacteria/yeast.

If you're not looking to grow a lot at once and need something discreet, this is a decent tek. It's less bothersome than PF tek because you fruit in vitro (no SGFC needed, no constant misting/fanning), so if you want to use brf and spore syringes, this is a good choice. I mostly used brown rice with casing and LC, and I PCd the jars. I did only a handful with BRF and spore syringes.

This tek also doesn't require coir (though you could certainly use it for the casing if you have some). I think that's a benefit. And the microwaved sifted potting soil casing Violet wrote up worked well. I never had it contam. The "never use a microwave in growing or things will go wrong" comments didn't line up with my experiences with that tek.

Another issue was the pp5 containers eventually warped, usually the lids, and if gripped too firmly, air from inside was pushed out and air from outside filled the vacuum. It was a contam risk when it happened during colonization. I ended up putting all my Glad screw-top containers in the recycle bin and going back to glass for monotub grows.

After my first monotub, I didn't return to this tek and don't think I ever will. Shoeboxes have better FAE and are easier to manage humidity and to harvest. I no longer recommend it to new growers unless they need discretion, can't source coir, or don't have a PC.

Edited by Moopers (10/23/22 04:08 AM)

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Offlinegun fuy
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Moopers]
    #28012090 - 10/23/22 08:10 AM (1 year, 6 months ago)

Ah yes I saw Muda's bottle tek and I've noted it to read through in more detail. Same for the shoeboxes, they sound pretty neat from the little I've read on them so far.

Actually the glass jars I've seen here are terrine jars so they don't taper in at the top. They are the brand Le Parfait "Familia Wiss". They have a two piece lid but the top piece that screws on doesn't have a big hole like some canning jars appear to have - it completely covers the "sealing" piece underneath.

For now I'm just looking at growing oysters and I know they're not suited to these kinds of teks. Still curious and enjoying learning about them. Thanks for the detailed replies :smile:


--------------------
"Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save." - RR

Edited by gun fuy (10/23/22 08:40 AM)

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OfflineUnicorncaca
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: gun fuy]
    #28018401 - 10/26/22 09:17 PM (1 year, 6 months ago)

Hey violet I have followed your instructions for rye grass seed and I have been having contamination issues. I have been having a hell of a time figuring out what was going on. I think I finally figured it out. I left some sterilized jars of seed for a few weeks and almost all of them started to mold . I pc'd for 90 minutes, not real sure where I'm going wrong. Also some of the plastic containers I knocked up have contam as well. Do you think I need to increase my PC time?

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Offlinebongoman
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Unicorncaca]
    #28018422 - 10/26/22 09:34 PM (1 year, 6 months ago)

You probably won't get a reply from Violet, she hasn't appeared here for some time now.

I would have thought that 90mins is sufficient for plastics — one of the advantages that Violet claimed for using PP5's was the shorter sterilisation time.

Could your lids be leaking too much air? Can you maybe wrap a test PP5 in Saran Wrap or parafilm around the lid and see if it contaminates?

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OfflineUnicorncaca
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: bongoman]
    #28018886 - 10/27/22 08:18 AM (1 year, 6 months ago)

Yes I'll give that a go, some of them were jars also. So frustrating I've never had problems like this with birdseed. I bought a 50# bag...

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OfflineMoopers
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Unicorncaca]
    #28019143 - 10/27/22 11:02 AM (1 year, 6 months ago)

I used the Glad brand 1L PP5 screw top containers and never had contam, but I used brown rice.

You are screwing the lids on tight after PC and after inoculation yes? With PODS tek, you don't partially open the lids until full colonization. No gas exchange is needed during colonization with this tek.

Are you venting your PC steam for 10 minutes before adding the weight?

If you're closing the lids tightly and are properly venting your PC, and PCing at 15psi for 90 mins (more than sufficient time for this tek, IME), then the contam is from your inoculant or your SAB work.

What are you using to inoculate: MSS, LC, agar?

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OfflineUnicorncaca
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Moopers]
    #28019770 - 10/27/22 04:23 PM (1 year, 6 months ago)

Lids were loose during pc and yes I vented until there was constant steam coming out. I may have left them loose after inoculation, I'm going to do another run tomorrow. Maybe I should soak overnight to help gid rid of the endospores? I'm using agar at the moment, and have been for awhile. I lost about 2/10 with birdseed and got many more flushes. I'm losing 7/10 now and I'm lucky if I get 1 flush before contam. Also the size of the fruit is smaller and less abundant.

Thanks a bunch!

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OfflineMoopers
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Unicorncaca]
    #28020335 - 10/27/22 08:49 PM (1 year, 6 months ago)

Once steam comes out full force (I go by when the pressure lock pops up), set a timer for 10 minutes and put the weight on when the timer is up. Otherwise, the inside of the PC won't come up to the right temperature.

See this post and this one for the reasons why.

You shouldn't be getting 20% contam rates on WBS if your cultures are clean (you're using agar, which goes a long way) and if your SAB work is sanitary. The only other place to look is PCing.

Re: endospores, here's a post with some science showing soaking won't help.

Best of luck! I hope you get your contam rates under control

Edited by Moopers (10/28/22 03:41 PM)

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