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InvisibleGnomenclature
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Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 1
    #27672220 - 02/24/22 07:56 PM (1 year, 10 months ago)

Thank you for the explanation! Excited to see how it goes.


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InvisiblerhizoRider
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Re: Path to fruiting ovoids "indoors" [Re: Gnomenclature] * 1
    #27672267 - 02/24/22 08:35 PM (1 year, 10 months ago)

:popcorn:


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Offlineworimi1
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Re: Path to fruiting ovoids "indoors" [Re: rhizoRider] * 1
    #27672322 - 02/24/22 09:17 PM (1 year, 10 months ago)

You can add honey to the LC list, 5ish ml to quart.
The mycelium is responding well and is thrown onto the stir plate every other day. I’ll update this post with a picture.

Looking forward to seeing how the rain/hail/storm simulation goes as much as reading the write up on the experiment.


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OfflineMycoplex
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Re: Path to fruiting ovoids "indoors" [Re: worimi1]
    #27672327 - 02/24/22 09:22 PM (1 year, 10 months ago)

Quote:

worimi1 said:
You can add honey to the LC list, 5ish ml to quart.
The mycelium is responding well and is thrown onto the stir plate every other day. I’ll update this post with a picture.

Looking forward to seeing how the rain/hail/storm simulation goes as much as reading the write up on the experiment.




Hey worimi1, I agree that honey lc is a great one.  I've tried this one too.  If you have some shots feel free to share, I am not good at photographing LC.  PS, I hope you saw your vegemite dishes on the front too.


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Offlineworimi1
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Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 1
    #27672511 - 02/25/22 12:39 AM (1 year, 10 months ago)

Yeah tops to see a posting of those pictures up front.
I’m starting to understand what you mean Mycplex, photographing liquid culture is difficult. I’ll try my best haha.


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OfflineMycoplex
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Re: Path to fruiting ovoids "indoors" [Re: worimi1]
    #27684289 - 03/05/22 07:47 PM (1 year, 10 months ago)

Hey fellas, has anyone had luck or interesting discoveries recently?

On my end I am still chugging along and following my same protocol for the indoor tubs.  The only thing that I decided to try differently starting yesterday was flooding every day rather than once a week or so. 

The reason I decided to start flooding the tubs every day was that I was struggling to find extra clues about what pinning triggers are for this species other than "rain," but after hopping on Wikipedia again (back to the basics) I noticed that it mentions their tendency to fruit after it has rained for several consecutive days, a common pattern in spring.  Ie, instead of one significant flood, perhaps a flood over several days with fresh water each time might make a difference?  This is the part that is different from what I've been doing so far, so I hope to at least stay busy with this even if it leads nowhere.

Ref: https://en.wikipedia.org/wiki/Psilocybe_ovoideocystidiata


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InvisibleGnomenclature
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Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 1
    #27684431 - 03/05/22 10:41 PM (1 year, 10 months ago)

Tried fruiting a cake post freeze/flood with filtered water. It just grew through the casing. Seeing your notes on several days of rain makes me wonder if using rain water would make a difference. Maybe the additional nitrogen source in the rainwater is a trigger?


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OfflineSingularFusion
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Re: Path to fruiting ovoids "indoors" [Re: Gnomenclature] * 1
    #27684472 - 03/06/22 12:04 AM (1 year, 10 months ago)

Hey dude

No further luck here unfortunately, even though temps came down to correct levels again, nothing turned up :kidreally:

Giving them a drying out cycle, see if anything can be teased out once they get wet again after that... Otherwise I will keep them around till autumn and hopefully nature will get them done then

Proceeding with outdoor expansion as normal but still have several things in various stages hanging around indoors, all cyan and azure mine is though of course, but some will stay indoors, some will go out, and some will be tubs that get nature for a couple of months but are essentially indoor tubs, a sorta hybrid model if you like

will let you know if anything happens

:chugbeer:


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OfflineSofaking420
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Re: Path to fruiting ovoids "indoors" [Re: SingularFusion] * 1
    #27689478 - 03/10/22 08:08 AM (1 year, 10 months ago)

Last year i did ovoids on popcorn and red wheat. Then i make shoeboxes 1:1 with hardwood blend of pellets for a pellet smoker grill. Once the shoe boxes where fully colonized i mixed them with more pellets and chips down by a small creek.

Ive checked them a few times over the winter when the snow melted and the myc is spreading and no animals have dug up that patch for grains.

Every other time my patches get dug up for the grains.

The pellets i treated the way i would coir. Boiling water into the coir bucket with pellets and slightly less then feild capacity.


Im really hoping to see some fruits this year


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OfflineMycoplex
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Re: Path to fruiting ovoids "indoors" [Re: Sofaking420]
    #27689708 - 03/10/22 11:58 AM (1 year, 10 months ago)

Quote:

Sofaking420 said:
Last year i did ovoids on popcorn and red wheat. Then i make shoeboxes 1:1 with hardwood blend of pellets for a pellet smoker grill. Once the shoe boxes where fully colonized i mixed them with more pellets and chips down by a small creek.

Ive checked them a few times over the winter when the snow melted and the myc is spreading and no animals have dug up that patch for grains.

Every other time my patches get dug up for the grains.

The pellets i treated the way i would coir. Boiling water into the coir bucket with pellets and slightly less then feild capacity.


Im really hoping to see some fruits this year




Hey Sofaking, best of luck to you this year.  If the patch survived last year, that must be a good sign.  Perhaps this Spring you'll start seeing some fruits.

I'm curious if the snow melting was significant here.  After the snow melted, what's the general moisture level of the patch a week or two later?  Thanks for sharing this!


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InvisibleInfinite Monkeys

Registered: 09/01/21
Posts: 276
Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 1
    #27694068 - 03/13/22 05:08 PM (1 year, 10 months ago)

Ovoids (same culture as previously posted) on potato/dextrose/honey agar.





The culture doesn't look terribly impressive on this type of agar. The culture is also displaying some type of contamination which didn't appear on any other type of agar.

The "clean" looking mea and millet agar plates got sent to grains and they tammed out quite quickly. It's interesting that the contamination was only apparent on PDHA.


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OfflineMycoplex
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Re: Path to fruiting ovoids "indoors" [Re: Infinite Monkeys] * 1
    #27694347 - 03/13/22 09:44 PM (1 year, 10 months ago)

Hey InfiniteMonkeys,

I'm thinking we can also add a "contam" section to the front that tracks some of the more common contams when cultivating ovoids.  I know I've personally seen cobweb a few times.  If you have any luck ID'ing what the contam is in the plate it would be nice to know.


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InvisibleInfinite Monkeys

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Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 1
    #27694401 - 03/13/22 10:27 PM (1 year, 10 months ago)

Quote:

Mycoplex said:
Hey InfiniteMonkeys,

I'm thinking we can also add a "contam" section to the front that tracks some of the more common contams when cultivating ovoids.  I know I've personally seen cobweb a few times.  If you have any luck ID'ing what the contam is in the plate it would be nice to know.




Hey mate.

Good idea.

The PDHA photos above are T5. The jars that were inoculated with T4 plates displayed a hint of green a few days in. Overnight, all of the grains turned green.

I threw the jars away rather than cleaning them :\

Trichoderma would be my best guess.

I'm going to start from spores again. This time, each transfer will be made as early as possible.

Since the contamination became obvious on the PDHA, I'll use that recipe again for T5 plates and see how the plates turn out.


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OfflineMycoplex
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Re: Path to fruiting ovoids "indoors" [Re: Infinite Monkeys]
    #27700893 - 03/19/22 12:05 AM (1 year, 10 months ago)

Hey guys, one thing that I experienced with indoor ovoid cultivation recently was one single quart jar of WBS which seemed to be colonizing way slower than the rest.  About 6 or 7 other jars were finished colonizing in about a month's time, but one was stuck at around 50%.

I feared contam obviously, but then I noticed a difference about this jar compared to the other, successful, ones.  The jar lid on the one straggler that was slow was using a syringe filter, whereas all the rest of the jars were using micropore tape as a filter.

I'm pretty sure that this exact thing has been brought up before and that this isn't the first time it's being written but when it comes to ovoids, and perhaps other woodlovers, having a restrictive jar filter (like a syringe filter) seems to slow and inhibit growth.

What I did to solve this was pry off the syringe filter (it's easy to do because RTV silicone is not really glue, it's easy to undo) and cover the hole with micropore.  I also 'burped' the jar in front of a flowhood for a couple seconds and after about 2 days the jar is proceeding to colonize the part that was untouched before.


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OfflineSingularFusion
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Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 2
    #27701171 - 03/19/22 09:31 AM (1 year, 10 months ago)

for sure way more air needed for smooth and fast colonization with these than cubes in my limited experience... they really seem to thrive on fresh air and the couple of bags I made did not fare well due to restriction of air I believe

At the time I made a big cut across the top of the ziplocks which I then taped over with micropore tape to make like a long filter strip on the bag. Was not enough in most cases and failed. Some of the fail may have been down to other things, but they really do like much air. That's about all I do know so far

Air, and also live compost and grasses or other plants seem to fire things up. I think I will be going more for a multi-layered sorta naturescape approach in future. Blackberry canes (brown ones), little twigs and stuff, soil/compost mixed throughout, not just as top layer. I have several intended refinements to attempt. But outdoor season looms and I am rushing to get things put together. Might even give up cube cult for half a year and just spawn endless WL projects till we win lol

shame they are so difficult indoors :sad: hope your luck proves better than mine mate


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Offlinesmalltalk_canceled
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Re: Path to fruiting ovoids "indoors" [Re: SingularFusion] * 2
    #27708282 - 03/25/22 03:12 PM (1 year, 9 months ago)

Joining this thread now, got the first grain jars of ovoids ready


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Willpower is the one true virtue



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OfflineMycoplex
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Re: Path to fruiting ovoids "indoors" [Re: smalltalk_canceled] * 1
    #27708318 - 03/25/22 03:54 PM (1 year, 9 months ago)

Hey welcome smalltalk.  What was your grain choice, and what are your plans for spawning the jars?  Looking forward to hearing from you.


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Offline7SunsS
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Re: Path to fruiting ovoids "indoors" [Re: Mycoplex] * 1
    #27711266 - 03/28/22 07:49 AM (1 year, 9 months ago)

Following :vaped:


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Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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OfflineKeefersutherland
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Re: Path to fruiting ovoids "indoors" [Re: 7Suns] * 2
    #27711704 - 03/28/22 03:42 PM (1 year, 9 months ago)

Got my first ovoid print coming from a giveaway. I’m getting on this immediately as soon as it arrives. I have a ton of leaf debris in my yard. Is this suitable to layer with wood chips in an outdoor bed? Just trying to find some uses for it.


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LAGM 2023


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InvisibleMysticMycologist
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Re: Path to fruiting ovoids "indoors" [Re: Keefersutherland]
    #27711800 - 03/28/22 05:16 PM (1 year, 9 months ago)

I think I read that ovoids really like dead grass, so I’m sure some leaf litter compost couldn’t hurt. Hope you enjoy the swabs. I can’t wait for everyone to be trying to fruit these. Let’s spread the spores far and wide! It is going to be a very exciting year.


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Two eyes to look, One eye to see.
Prying open my third eye


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