Home | Community | Message Board

World Seed Supply
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Offlineyas champ
Stranger
 User Gallery
Registered: 06/27/19
Posts: 107
Last seen: 3 hours, 54 minutes
how to solve contamination problems
    #27557078 - 11/25/21 04:59 AM (3 days, 9 hours ago)

Hello, I prepared a medium using MEA with an infusion of straw instead of normal water (I boiled straw in water) and I had this result in 24 hours almost 48 hours, I know that it is impossible to have a mycelium in such a short time, I would like to have your opinion please?
ps: mushroom used is agaricus bisporus

     


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleWrightii
Stranger Danger
I'm a teapot User Gallery


Registered: 05/15/20
Posts: 149
Re: how to solve contamination problems [Re: yas champ]
    #27557081 - 11/25/21 05:09 AM (3 days, 8 hours ago)

Did you go from agar to agar? And did you sterilize the whole bunch? Indeed a bit sus that this happens in 24 hours, but maybe A. bisporus just does that?
I mean it could be cobweb I guess but could be whispy mycelium just as well.
At least, thats how far my reasoning goes


--------------------
The Official Hericium Thread


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineyas champ
Stranger
 User Gallery
Registered: 06/27/19
Posts: 107
Last seen: 3 hours, 54 minutes
Re: how to solve contamination problems [Re: Wrightii]
    #27557086 - 11/25/21 05:28 AM (3 days, 8 hours ago)

no it's from a fungus to the agar, and I sterilized the medium for 30min at 121° C, precisely, for the agaricus it usually takes me between 15 to 20 days for an average growth, so for me it's rather a contamination, and I wanted to confirm.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleAssyrian
Stranger
Registered: 11/17/21
Posts: 156
Re: how to solve contamination problems [Re: yas champ]
    #27557175 - 11/25/21 08:14 AM (3 days, 5 hours ago)

Quote:

yas champ said:
no it's from a fungus to the agar, and I sterilized the medium for 30min at 121° C, precisely, for the agaricus it usually takes me between 15 to 20 days for an average growth, so for me it's rather a contamination, and I wanted to confirm.




Did you do a reasonably clean transfer from mushroom to agar?
Best would be to split open a mushroom stem inside a still air box by tearing it apart (not cutting it), then use some sterilized tweezers to get a piece of the inside of the stem and transfer that to agar.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineForresterM
embracing the subtle
Male User Gallery

Registered: 02/05/13
Posts: 7,201
Loc: Maine, USA Flag
Last seen: 3 hours, 46 minutes
Trusted Cultivator
Re: how to solve contamination problems [Re: Assyrian]
    #27557180 - 11/25/21 08:24 AM (3 days, 5 hours ago)

Quote:

Assyrian said:
Best would be to split open a mushroom stem inside a still air box by tearing it apart (not cutting it), then use some sterilized tweezers to get a piece of the inside of the stem and transfer that to agar.




:whathesaid:


--------------------
Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineyas champ
Stranger
 User Gallery
Registered: 06/27/19
Posts: 107
Last seen: 3 hours, 54 minutes
Re: how to solve contamination problems [Re: Forrester]
    #27557250 - 11/25/21 09:23 AM (3 days, 4 hours ago)

I work between two bunsen burners and I cut the small piece of mushroom with a sterile scalpel, that's why I don't really understand the source of this contamination. but I never tried to make pieces inside the stem I usually take at the cap.
otherwise i hear about the glove box but i'm not so sure about the sterility, the air inside is not sterile, how to sterilize plastic?


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleAssyrian
Stranger
Registered: 11/17/21
Posts: 156
Re: how to solve contamination problems [Re: yas champ]
    #27557258 - 11/25/21 09:35 AM (3 days, 4 hours ago)

Quote:

yas champ said:
I work between two bunsen burners and I cut the small piece of mushroom with a sterile scalpel, that's why I don't really understand the source of this contamination. but I never tried to make pieces inside the stem I usually take at the cap.
otherwise i hear about the glove box but i'm not so sure about the sterility, the air inside is not sterile, how to sterilize plastic?




Air inside doesn't have to be sterile, it's just to prevent dust particles to fall and move around.
It won't be as good as a flow hood but it's still better than working between two bunsen burners


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleWrightii
Stranger Danger
I'm a teapot User Gallery


Registered: 05/15/20
Posts: 149
Re: how to solve contamination problems [Re: Assyrian]
    #27557268 - 11/25/21 09:51 AM (3 days, 4 hours ago)

Dude, you wrote like 130 posts over the last 8 days. Which are also you're first 8 days as a new member. Pretty impressive!:ooo: Were you a lurker before? Thats some steep learning curve:tongue:


--------------------
The Official Hericium Thread


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineyas champ
Stranger
 User Gallery
Registered: 06/27/19
Posts: 107
Last seen: 3 hours, 54 minutes
Re: how to solve contamination problems [Re: Assyrian]
    #27557304 - 11/25/21 10:16 AM (3 days, 3 hours ago)

ok thanks for the advice:smile:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleAssyrian
Stranger
Registered: 11/17/21
Posts: 156
Re: how to solve contamination problems [Re: Wrightii]
    #27557307 - 11/25/21 10:19 AM (3 days, 3 hours ago)

Quote:

Wrightii said:
Dude, you wrote like 130 posts over the last 8 days. Which are also you're first 8 days as a new member. Pretty impressive!:ooo: Were you a lurker before? Thats some steep learning curve:tongue:




Been a lurker on and off for a very long time, then just realized there are parts of this website that cannot be accessed if you aren't registered :facepalm:


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineyas champ
Stranger
 User Gallery
Registered: 06/27/19
Posts: 107
Last seen: 3 hours, 54 minutes
Re: how to solve contamination problems [Re: Wrightii]
    #27557310 - 11/25/21 10:20 AM (3 days, 3 hours ago)

yes I was a former lurker lol, I stopped a while ago, since I had no conclusive results, but now I'm back for some tests hoping it will be positive :smile:


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineForresterM
embracing the subtle
Male User Gallery

Registered: 02/05/13
Posts: 7,201
Loc: Maine, USA
Last seen: 3 hours, 46 minutes
Trusted Cultivator
Re: how to solve contamination problems [Re: yas champ]
    #27557411 - 11/25/21 11:38 AM (3 days, 2 hours ago)

Quote:

yas champ said:
I work between two bunsen burners and I cut the small piece of mushroom with a sterile scalpel, that's why I don't really understand the source of this contamination. but I never tried to make pieces inside the stem I usually take at the cap.
otherwise i hear about the glove box but i'm not so sure about the sterility, the air inside is not sterile, how to sterilize plastic?




You work between bunses burners just in open air?  That's why you're getting contamination.

Yes you need a still air box, all you do is wipe down the inside with alcohol and let it sit for a while with all your work inside ready to go.  Then spores from the air don't settle onto what you're working on.

It's important to rip the stem apart, because if you cut into it you're dragging bacteria from the outside of the stem into your sample.  Just rip apart the stem, then use tweezers or a scalpel to take a sample from the inside, place on agar and close the lid.  You should get much better results.


--------------------
Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineyas champ
Stranger
 User Gallery
Registered: 06/27/19
Posts: 107
Last seen: 3 hours, 54 minutes
Re: how to solve contamination problems [Re: Forrester]
    #27560221 - 11/28/21 03:18 AM (10 hours, 43 minutes ago)

thank's for all these tips:smile:


Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* problems cloning wild enoki ... ragadinks 3,718 14 01/19/04 08:52 PM
by r05c03
* natural gas multi-jet burner msmushrooms 1,627 3 05/10/05 04:39 PM
by msmushrooms
* Straw problems MushMushi 1,637 5 01/07/03 08:53 PM
by zeronio
* agar problems taters 1,340 5 05/04/03 11:17 AM
by taters
* Pet hair and contamination? MizzJazzy 1,218 8 11/22/09 09:35 PM
by Mephistophelian
* Does anyone cultivate/make Tempeh?
( 1 2 3 all )
MycoCultures 8,103 52 07/15/05 04:03 PM
by ShroomOmatic
* Oops. I guess wood can contaminate. Baby_Hitler 1,392 4 04/14/03 11:54 PM
by falcon
* paddy straw mushroom - culture contaminated by coprinus drzira 3,076 12 08/12/04 03:25 AM
by drzira

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, wildernessjunkie, cronicr, Pastywhyte, Forrester, Stromrider
118 topic views. 4 members, 4 guests and 3 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.036 seconds spending 0.007 seconds on 13 queries.