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Amazon Shop for: Agar, Autoclave

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OfflineSrPompon
Satanicus Fungis
Registered: 05/31/04
Posts: 2
Loc: Argentina
Last seen: 12 years, 10 months
Medical Sterization Oven 0-572 Farenehits
    #2754931 - 06/01/04 11:47 PM (12 years, 10 months ago)

Hello,

I want to start my first grow from spore-prints.
I have a medical sterization oven 0 to 572 Farenehits, what amount of time and temp y need for a correct esterilization of agar+malt+extract? (i get this incubation media from a quimical suplier)

Thanks,

Mr. Pompon


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Offlinetripin420
jus chillin
Registered: 05/20/03
Posts: 170
Last seen: 12 years, 9 months
Re: Medical Sterization Oven 0-572 Farenehits [Re: SrPompon]
    #2754945 - 06/01/04 11:51 PM (12 years, 10 months ago)

whoa them sterization ovens are a pretty penny if me and u talking bout the same unit were talking like 900-1200 buxs my tat guy has one for all his tat needles


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Offlineyeehaw
Always SmoothNever Bitter

Registered: 05/05/04
Posts: 118
Last seen: 11 years, 28 days
Re: Medical Sterization Oven 0-572 Farenehits [Re: SrPompon]
    #2755380 - 06/02/04 01:52 AM (12 years, 10 months ago)

If it is an autoclave then it really just depends on the size of what you are sterializing.

At 121 degrees Celcius (15 psi) it takes 15 minutes to completely sterlialize a 10 ml test tube, 15 minutes for a 95 mL flask, 30 minutes for a 1500mL flask, 70 minutes for a 6750 mL fermentation bottle.

This was copied straight from a microbiology book...


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OfflineSrPompon
Satanicus Fungis
Registered: 05/31/04
Posts: 2
Loc: Argentina
Last seen: 12 years, 10 months
Re: Medical Sterization Oven 0-572 Farenehits [Re: yeehaw]
    #2756450 - 06/02/04 12:44 PM (12 years, 10 months ago)

Ok.. but at 125C for 30 minutes i dont "cook" the sustrate? like a pastel?


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Amazon Shop for: Agar, Autoclave

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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