Home | Community | Message Board

Magic Mushrooms Zamnesia
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: < Back | 1 | 2 | 3  [ show all ]
OfflineForresterM
aspiring sociopath
Male User Gallery

Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 23 days, 21 hours
Trusted Cultivator
Re: Myco Parasites [Re: Kizzle] * 1
    #27560425 - 11/28/21 07:57 AM (2 years, 1 month ago)

Quote:

Kizzle said:
Also in all my encounters with mycogone-like fruit body deformations I've never seen any indication of a disease spreading. They usually appear in late colonization or early pinning and then normal fruit bodies follow.




FWIW, I've only run into mycogone infections a couple times, both with edible or medicinal species.

I think once was king oysters, and that time the substrate only produced one, giant MP bubble.  No other fruit bodies ever formed before or after that.  I left it to sit quite a while with the giant bubble because I didn't know at the time what it was.  Never fruited.

I think the other time may have been with reishi.  Again, IIRC no reishi fruits ever were produced, the MP seemed to entirely prevent it from doing so.

:shrug:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Myco Parasites [Re: Forrester] * 1
    #27560465 - 11/28/21 08:34 AM (2 years, 1 month ago)

This is my experience as well, nof fruits, no pins.


Extras: Filter Print Post Top
OfflinexVadisx
MountainsDogsAndFungi
Male


Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
Last seen: 10 hours, 19 minutes
Re: Myco Parasites [Re: QM33]
    #27580963 - 12/14/21 09:52 AM (2 years, 1 month ago)

I've been dealing with a series of fucked over the past few days that I suspect is some real nasty nasty. I've posted in a few places but sandman asked me to pop this over here so here are some quotes:

Quote:

xVadisx said:
It was near a few bags that looked like this:


Several rosecomb mushrooms. A few with early broken veils, rosecomb heads, and dark ripped-looking (but not split) stipes. Lots of dry-bubble-looking/yellow/blobby stuff. I saw it in ziplocks and from a cake of the same variety. I've thrown away the worst in fear.

I've isolated everything else outside the grow area and am letting things go and debating harvesting/using. I probably will go ahead and let them grow and harvest at least a first flush...I just don't want to fuck my upcoming grows/plates/work area. I have six PF cakes that I already took some tissue to agar from (a super spikey cake) but am now hesitant to continue with as I don't want to transfer ick.

I despise losing actives, but with so many things coming up that are clean, I want to keep them clean and am not that attached. I still don't want to waste unnecessarily. I have a shoebox and two ziplocks as well that looks much much healthier but were from the same timeframe/CV-batch.

So, the grow room just has jars of oats and plates and has been cleaned. Air filter is running for whatever its worth. Everything else is in a completely other room. I'm not going to mist the cakes any more. They do what they do now. One is doing a pushup.






Quote:

xVadisx said:
Is it okay to dehydrate bacterial fruit at 165F?

Logically, there is a fan... and it will blow hot air at these things moving air around (and I'm mostly curious if this air will then bring clouds of invisible-to-the-naked-eye-fuckedness to my home, a scientific term).

I haven't flipped the switch yet. I hate tossing actives.






If it is as nasty as I think I'm paranoid about spores getting everywhere, carried on me, etc. Gah.


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Myco Parasites [Re: xVadisx]
    #27670149 - 02/23/22 07:31 AM (1 year, 10 months ago)

Quote:

In contrast to viruses of plants and animals, mycoviruses uniformly lack an extracellular phase to their replication cycle.
Consequently, they are not infectious in the classical sense. Infections
cannot be initiated by exposure of uninfected hyphae to cell extracts
prepared from an infected fungal strain. Rather, mycoviruses are transmitted by intracellular mechanisms such as anastomosis (fusion of
hyphae) or through asexual spores.




Can anyone help me understand this?

Quote:

Both protocols require the
generation of cell-wall-free spheroplasts from virus-free C. parasitica strains. For transformation, a plasmid, that contains a full-length hypovirus cDNA copy and an independent selectable marker gene, is introduced into C. parasitica spheroplasts by DNAmediated transformation. Transformants that contain the chromosomally integrated
plasmid and cDNA-derived cytoplasmically replicating hypovirus RNA are selected
following cell-wall regeneration and growth in the presence of the appropriate antibiotic (Choi and Nuss, 1992). The hypovirus transfection system uses synthetic transcripts
corresponding to the hypovirus coding strand RNA (12.7 kb in the case of hypovirus CHV1/EP713) that are synthesized in a T7-polymerase-dependent cell-free transcription
system. The synthetic transcripts are introduced into spheroplasts by electroporation
and followed by cell-wall regeneration in the absence of any selection (Chen et al., 1994).
Replicating hypoviruses are able to migrate through the cytoplasmic network of the
regenerated hyphal colony.




You see, I'm not very bright but I have the spirit! I gots moxy!

Quote:

Mycoviruses are able to readily spread through the hyphal network that
comprises a fungal colony.




from here

I think this means that only spores from infected hyphae and the hyphae fusing can spread the virus? How does it start then? I don't fully grasp what this means.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (02/23/22 07:36 AM)


Extras: Filter Print Post Top
Offlinerockyfungus
dirty
 User Gallery

Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 19 days, 12 hours
Re: Myco Parasites [Re: sandman420] * 1
    #27670169 - 02/23/22 08:07 AM (1 year, 10 months ago)

I'll keep it short as I'm not 100% sure on the language and only read your excerpts. Also nothing is a "virus" in the study. It's a parasite they took genetic info from and inserted into a bacteria (plasmid)??

Mycoviruses don't traditionally hijack a cell extracellularly. Viruses typically find a receptor attach and inject genetic material.

Fungal viruses are active in hyphael networks and spores? How do they get in?

The 2nd blurb is how they got the genetic material in. They had to destroy the cell walls of a parasite so it could take up genetic info and form spheroblasts. Sphereoblasts could only enter a (damaged?) hyphal network. They destroyed the hyphaes and rebuilt them?
Which I suppose this parasite is resembling the mycovirus to prove they can only infect through those processes?

So where in growth does hyphal colony have shedded cell walls that can be infected. Or how do spores get infected?
Basically is there a place in fungal reproduction where it's hyphael network is bare and exposed?

Rambled if you have a direct question I can try and break it down. Or if you want a term broken down. I hate explaining stuff to other mycologist like they don't understand science. I've done a fuck ton of biology so some words are standard. The more educated people get in a singular field the less they understand what normal people know.
So if you need it explained like your 5. No worries I'll try.


Edited by rockyfungus (02/23/22 08:28 AM)


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Myco Parasites [Re: rockyfungus] * 1
    #27670246 - 02/23/22 09:32 AM (1 year, 10 months ago)

By asexual spores do they mean chlamydospores?


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Myco Parasites [Re: QM33]
    #27670251 - 02/23/22 09:43 AM (1 year, 10 months ago)

I know that the uhh..spores of infected hyphae carry the virus so it spreads that way. I recall reading one paper that said something to the effect of 80%+ of spores from the infected fungi have the virus.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Myco Parasites [Re: sandman420]
    #27670269 - 02/23/22 10:09 AM (1 year, 10 months ago)

""Rather, mycoviruses are transmitted by intracellular mechanisms such as anastomosis (fusion of
hyphae) or through asexual spores.

I mean basidiospores are not asexual right?

I mean this is what I got for 5 seconds on Google

Are Basidiospores asexual? No. The life cycle of Basidiomycota can be divided into two phases – sexual and asexual. Basidiospores are used in sexual reproduction.Jan 14, 2019
https://www.bustmold.com › resources


Idk this might be worth a read
https://www.sciencedirect.com/topics/immunology-and-microbiology/asexual-spore


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Myco Parasites [Re: QM33]
    #27670296 - 02/23/22 10:37 AM (1 year, 10 months ago)

Ok well I think the hangup is on that one paper simply using the word asexual spores there. They may have been specifically referring to the fungi in question on that paper, I think it was a rust blight or something and guessing it makes chlamydospores?

It definitely spreads through the spores of mushrooms, which correct me if I'm wrong would be basidiospores right.

Quote:

While sharing some characteristics with animal and plant viruses, mycoviruses also have the following unique characteristics: (1) most mycoviruses lack an extracellular route for infection; (2) mycoviruses are transmitted intercellularly only through cell division, sporulation, and cell fusion; and (3) mycoviruses apparently lack a movement protein, which is essential for the life cycle of animal and plant viruses.




On this paper they don't specify asexual spores.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (02/23/22 10:49 AM)


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Myco Parasites [Re: sandman420]
    #27670318 - 02/23/22 11:13 AM (1 year, 10 months ago)

Now I present to you exhibit Q

I believe this sandbag to be infected with a mycovirus or mycoparasite.

This is just an example of my last run of several sandbags, every bag was identical to this pretty much.

The culture was a plate pin clone culture that looked, not to toot my own horn but it looked way better than anything you'll ever grow in your life sucka :crazy2: :rocket: :evil:...

It's natalensis.

The plates were so clean.

LC clean.

Grain was clean but ~80% of the bags stalled.

The grains that finished looked fine, they all looked fine but they would stall after shaking. No stank. No bahturia even on the stalled ones.

So i spawned my few sandbags with the good grain and they colonized mostly ok. Took a long fucking time to pin.

Heres what we gots homey...

Mostly less than 1" tall. NO SPORES. Very shitty. The plate clone was a huge spaghetti monster and sporulated LIKE CRAZY on the plate

Now you may say just genetics but fuck no man. This same shit has been happening to everything all kinds of cultures of cubensis too, getting worse every month till now I think it's hit max fucky.

I know that mycovirus (and probably mycoparasite too) effect the sporulation. I have been getting so many first flush no spores on so many projects.



So I know I have a huge fucking problem. I don't know how far it goes is the problem. I think everything in my culture storage is carrying this. I think all of my spores carry this.

I am shutting down my room, selling out, moving, ordering fresh spores from a virgin monk in Indonesia, and starting fresh. It's the only way to be sure.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (02/23/22 03:18 PM)


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Myco Parasites [Re: sandman420] * 1
    #27670322 - 02/23/22 11:15 AM (1 year, 10 months ago)

:batlol::baaaam:


Extras: Filter Print Post Top
OfflineForresterM
aspiring sociopath
Male User Gallery

Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 23 days, 21 hours
Trusted Cultivator
Re: Myco Parasites [Re: sandman420] * 1
    #27670323 - 02/23/22 11:16 AM (1 year, 10 months ago)

Wow, that's insane what you've got going on there.  Eesh...


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Myco Parasites [Re: Forrester]
    #27670330 - 02/23/22 11:29 AM (1 year, 10 months ago)

:fuck:

I wanted to move anyway.:feelsbatman:

But heres the thing, this is a fresh print that was sent to me from someone and I just worked with it on agar in the flowhood besides open air spawning so how the fucks did I get this fuckin fuck?! Fuck.

I would be lead to believe the mycoviruses are not air transmittable by my new learning. But not mycoparasites to my knowledge.

The fuck is going on.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
Offlinerockyfungus
dirty
 User Gallery

Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 19 days, 12 hours
Re: Myco Parasites [Re: sandman420] * 1
    #27670355 - 02/23/22 12:00 PM (1 year, 10 months ago)

Someone should send you a known culture already colonized, see what happens?
Or flip. Work somewhere else with fresh clothes, tools, print. Fruit at normal place and elsewhere and compare?

Or you just picked a turd or need to dial those conditions in :grin:


Extras: Filter Print Post Top
OfflineDendrocopos
Latin woodpecker
I'm a teapot


Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
Re: Myco Parasites [Re: rockyfungus]
    #27675408 - 02/27/22 11:50 AM (1 year, 10 months ago)

Repost from agar envy thread. Sorry if that is not allowed. My cultures have all started to look kind off funky ever since i bough some maz spores. I get crazy rosecomb/mutants on everything i grow. Along weird mycogone like blobs of stem tissue oozing metabolites. Mr. Sandman told me i most likelly have some sort of mycovirus/mycoparasite based on my plates. Could someone give me a second opinnion before i toss all my shit ? Or, how does one get rid of something like that without tossing everything ?

Looks good, but rhyzomorphs weirdly lift into the air like they are escaping from something.



weirdly ununiform growht



3D/fuzzy look. Looks like bacterial bubbling.



?clean plate? i am so paranoid, i don't know at this point



Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Myco Parasites [Re: Dendrocopos]
    #27675630 - 02/27/22 02:27 PM (1 year, 10 months ago)

Bacteria as a mycoparasite is my main interest right now. We need more information on that. I bet it's a big one.

I think your last plate is looking good.

But your other picture sure do show a lot of intersting stuff that if linked to your problem could be handy for others.

First pic the multiple layers is odd. The tendril like upcropping's in the middle are noteworthy.

2nd picture the petering off and confused unorganized look is noteworthy.

3rd picture I mean dang what's going on with the grainy spots?

Love to hear someone else talk about this for a minute too :tomatoface:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
Offlinerockyfungus
dirty
 User Gallery

Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 19 days, 12 hours
Re: Myco Parasites [Re: sandman420]
    #27676041 - 02/27/22 07:32 PM (1 year, 10 months ago)

Unrelated but possibly related? Was reading about the parasites I may come in contact with in my line of work.
The trophozoites are mobile and consume bacteria (which allows for the diagnosis on E. coli plates).  The trophozoites form double walled cysts which are incredibly resistant to methods of eradication (including freezing, heating, and irradiation).

Not going to dig but do they eat fungi?

https://en.wikipedia.org/wiki/Trophozoite


Extras: Filter Print Post Top
InvisibleGandalfTheWhite
Newb


Registered: 05/02/21
Posts: 84
Re: Myco Parasites [Re: Stipe-n Cap]
    #28006984 - 10/19/22 07:57 PM (1 year, 3 months ago)

Saw that there was a couple posts in General Disc. in regards to this subject, but then noticed this thread was created. Apologies for resurecting an older thread.

Dealing with something quite similar with my APE culture. Swabbed a few plates, put some cotton fibers on the plate, snapped the tip off on a couple dishes and nothing looks right.

This is one of the T1's from the germ plates that I made early on. As you can see the scalloping and disorganized growth is obvious. Even the germ plates are starting to go south with discoloration and some bruising of the myc.



Think this culture is going to be a wash and will have to start over with another source


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Growth Inhibitory Compound Discussion (BLIS-Chitinase) [Re: GandalfTheWhite]
    #28007081 - 10/19/22 08:57 PM (1 year, 3 months ago)

Classic floccose appearance with scalloped edge, I don't personally even touch those unless I plan on scoping it; considering I don't yet own a scope....do you own one?


Extras: Filter Print Post Top
InvisibleGandalfTheWhite
Newb


Registered: 05/02/21
Posts: 84
Re: Growth Inhibitory Compound Discussion (BLIS-Chitinase) [Re: Stipe-n Cap]
    #28007229 - 10/19/22 10:40 PM (1 year, 3 months ago)

Thats kind of my plan ever since I noticed all this ragged growth. I've been searching around the past few days trying to find something decent that would work for this type of application.
Feel like a stereo scope would be good for looking at plates and early transfers, but for this I would need a compound scope to see whats going on.
I'd really like to look into stuff like this even though I could easily toss the plate and start over, but this has me curious.

My current dilemma being I want both.. at some point. Just not sure where to start comparing all the different specs or whats decent/trash


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* I influenced my partners mushroom growth with meditation! enotake2 1,579 16 02/09/03 04:42 PM
by z@z.com
* Gypsum and Drywall Compound nettwerk 2,139 5 03/21/03 09:32 AM
by nettwerk
* Which of these pan cyan growth patterns is preferable? Eminence 339 15 10/23/22 03:47 PM
by Eminence
* No mycelium growth sammyem1 150 5 10/16/22 04:41 PM
by sammyem1
* Now slow fuzzy jar growth andriscus 923 2 11/03/03 07:18 PM
by andriscus
* Tell me about pH and its role in mushroom growth World Spirit 1,067 3 02/10/02 04:07 PM
by Azure
* Compounding pharmacies are tremendous! Anonymous 581 1 01/10/04 09:59 AM
by Yarry
* .
( 1 2 all )
dr_gonz 7,032 25 12/08/04 11:46 AM
by scatmanrav

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,682 topic views. 16 members, 156 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.011 seconds on 14 queries.