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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 2 years, 20 days
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Re: Cultivation General Discussion [Re: TedsDead]
#27519000 - 10/26/21 02:44 PM (2 years, 4 months ago) |
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Quote:
Quirkmeister92 said: I noc dozens in a session and Ive been wiping the bag down, cutting the top off, innoculating, then sealing each double.
I'm gonna inoculate like 30 bags today, 9 are not presealed, but I'm gonna wipe em down all the same.
I run my FFU constantly, and I always wipe.
Quote:
TedsDead said: yeah, definitely a good idea if your gonna let em sit for any amount of time
I am not trying to argue wether to wipe or not, i am just sick of the amount of alcohol I use and I kind of feel that wiping with bleachy water would essentially do the same thing as wiping with iso. I still use plenty of iso for surfaces and other stuff, but I just can't deal with the smell anymore when mass inoculating bags. I think iso is overused and only gives us a false sense of security and maybe just wiping the floaters is just as good as killing (99%) of them. In the next weeks i will start to run some experiments and see whether iso really makes that difference. I thought I ask first what the current consensus is.
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TedsDead



Registered: 01/03/17
Posts: 4,999
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Re: Cultivation General Discussion [Re: eLeSDenes]
#27519005 - 10/26/21 02:54 PM (2 years, 4 months ago) |
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iso evaporates so u wont be sealing a wet bag which doesnt always work so well
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 18 days
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Re: Cultivation General Discussion [Re: TedsDead]
#27519046 - 10/26/21 03:31 PM (2 years, 4 months ago) |
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I agree about fast evaporation being nice. And it's definitely not the consensus, but I use ethanol-based sanitizer so I can inhale as much as I like.
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JohnnieYen
Okay



Registered: 03/15/11
Posts: 3,529
Loc: City Z
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Re: Cultivation General Discussion [Re: eLeSDenes] 3
#27519059 - 10/26/21 03:46 PM (2 years, 4 months ago) |
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Quote:
eLeSDenes said: People who do lots of bags:
So I am going through lots of bags, I pre-seal, PC and set on the shelf in the lab and inoc them when I have 100-200. They don't really get dirty (as it's a clean room with flowhood always running) but I always like to iso spray the top of the bags and wipe before I cut it to sanitise them. You can imagine the fume this process creates after you do like 100 bags.
SO not only this is very wasteful and potentially dangerous, but I think it's completely overkill. As an alternative, I was thinking about a bucket with 10% bleach water (tbh soapy water would work too) and dip the bags in for like a second to wash off any floaters. Does anyone do this? What do you guys do?
I didn't really let bags sit out more than a day and I never pre sealed them either. If I couldn't inoculate I would just leave them in the pc until I was ready. I just sanitized my gloves, not the bags. I had a lot of pc's (fifty 5lb bags per run) I had 3 x 941, 2 x 930, 3 presto 23quart, and a 75x

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TedsDead



Registered: 01/03/17
Posts: 4,999
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Re: Cultivation General Discussion [Re: JohnnieYen]
#27519102 - 10/26/21 04:16 PM (2 years, 4 months ago) |
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ya, I wouldnt wipe the bags unless they were sitting out on the flood or something
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: JohnnieYen]
#27519104 - 10/26/21 04:17 PM (2 years, 4 months ago) |
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You use propane, in the house, that close to a flammable wall!?
Blowing my mind man, wish I could do something like that.
Why don't u use electric?
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JohnnieYen
Okay



Registered: 03/15/11
Posts: 3,529
Loc: City Z
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Re: Cultivation General Discussion [Re: QM33]
#27519115 - 10/26/21 04:26 PM (2 years, 4 months ago) |
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Quote:
QM33 said: You use propane, in the house, that close to a flammable wall!?
Blowing my mind man, wish I could do something like that.
Why don't u use electric?
It wasn't my house, and the landlord saw my setup. He was already charging me more than he should for running my 75x and electric heat. You can't see it from the pic but I had french doors that were open and a 24" industrial fan exhausting. And a window open on the opposite end, so the airflow was quite good. I had it under control and someone was always there with a fire extinguisher.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 11 hours
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Re: Cultivation General Discussion [Re: TedsDead]
#27519120 - 10/26/21 04:28 PM (2 years, 4 months ago) |
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Quote:
TedsDead said: ya, I wouldnt wipe the bags unless they were sitting out on the flood or something
Exactly where I like to keep mine
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JohnnieYen
Okay



Registered: 03/15/11
Posts: 3,529
Loc: City Z
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Re: Cultivation General Discussion [Re: Failboat]
#27519140 - 10/26/21 04:52 PM (2 years, 4 months ago) |
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Can I get your guys opinion on this concept. I got a lot of reddit hate (like a lot) from noobs when I suggested it. https://www.shroomery.org/forums/showflat.php/Number/27506021
TL;DR its starting cultures by using mini grain jars to get myc started, then transferring a colonized grain to agar. The argument is why not just start with a drop to agar, its just an extra step and another chance of contamination. My argument is that i previously used these same syringes to agar and got nothing but bacteria after waiting 3 weeks. dishes pictured in the thread are 15 days from inoculation of grain. 10 days after inoculation the transfer was put to agar and it grew out for 5 days.
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TedsDead



Registered: 01/03/17
Posts: 4,999
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Re: Cultivation General Discussion [Re: JohnnieYen] 1
#27519152 - 10/26/21 05:00 PM (2 years, 4 months ago) |
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if it works it works but why take the syringes to grain if they were already bacterial on the plates? u want to try to get some germination and then clean it up after you put it to agar?
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 2 years, 20 days
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Re: Cultivation General Discussion [Re: Failboat]
#27519153 - 10/26/21 05:02 PM (2 years, 4 months ago) |
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yeah, unfortunately I can't keep them in front of the flowhood - i just stack them on shelves until I have like 100. Before using them I wipe the top of the bags with iso then cut them open. I spend quite a lot of time in front of the flowhood and my success rate is good but the amount of iso fume i breathe makes me gag every time and just made me question whether iso is even doing anything.
Next time I will try a 10% bleach solution to spray and wipe bags on 1 round of bags, and a soap + water on the other.
I am not trying to cut corners here I just think we are religiously using too much iso:D
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JohnnieYen
Okay



Registered: 03/15/11
Posts: 3,529
Loc: City Z
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Re: Cultivation General Discussion [Re: TedsDead]
#27519162 - 10/26/21 05:08 PM (2 years, 4 months ago) |
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Quote:
TedsDead said: if it works it works but why take the syringes to grain if they were already bacterial on the plates? u want to try to get some germination and then clean it up after you put it to agar?
i was waiting 3 weeks and not getting germination, just bacteria. For me this worked faster and I got clean plates after the grain transfer
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TedsDead



Registered: 01/03/17
Posts: 4,999
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Re: Cultivation General Discussion [Re: JohnnieYen] 1
#27519209 - 10/26/21 05:54 PM (2 years, 4 months ago) |
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if it works good for ya and is repeatable then screw what people on reddit say
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 11 hours
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Re: Cultivation General Discussion [Re: Inocuole]
#27519231 - 10/26/21 06:14 PM (2 years, 4 months ago) |
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No seal bags are nice... definitely making the switch.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,212
Last seen: 10 hours, 42 minutes
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Re: Cultivation General Discussion [Re: Failboat]
#27519271 - 10/26/21 06:48 PM (2 years, 4 months ago) |
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what are the reasons to seal bags when using a steamer? Does a steamer not cool down fast enough to make a vacuum seal?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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TedsDead



Registered: 01/03/17
Posts: 4,999
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Re: Cultivation General Discussion [Re: tedoro]
#27519319 - 10/26/21 07:24 PM (2 years, 4 months ago) |
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it makes a seal but it takes a very long time to cool down and could possibly introduce contams. maybe u can get away with not doing it but its what I do. plus I cant fit 50 bags in front of my hood at once so it gives me extra peace of mind knowing that they're fine sitting out of the flow
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 11 hours
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Re: Cultivation General Discussion [Re: TedsDead]
#27519879 - 10/27/21 08:46 AM (2 years, 4 months ago) |
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I tried bucket tek again, and I think I like it. Definitely limited by the water heater it seems 4 or 5 really good ones back to back, but sunce it's prep work I can just do sets of 4 and stack em up. Got 10 to spawn now and I'll prep the next 10 today. Getting caught up
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,884
Last seen: 13 hours
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Re: Cultivation General Discussion [Re: Failboat]
#27519905 - 10/27/21 08:58 AM (2 years, 4 months ago) |
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In the market for 100 L water boiler fo' sho
-------------------- Willpower is the one true virtue
  
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,884
Last seen: 13 hours
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I think I'm going crazy..
-------------------- Willpower is the one true virtue
  
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 6 months
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Re: Cultivation General Discussion [Re: JohnnieYen] 1
#27520165 - 10/27/21 12:30 PM (2 years, 4 months ago) |
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Quote:
JohnnieYen said: Can I get your guys opinion on this concept. I got a lot of reddit hate (like a lot) from noobs when I suggested it. https://www.shroomery.org/forums/showflat.php/Number/27506021
TL;DR its starting cultures by using mini grain jars to get myc started, then transferring a colonized grain to agar. The argument is why not just start with a drop to agar, its just an extra step and another chance of contamination. My argument is that i previously used these same syringes to agar and got nothing but bacteria after waiting 3 weeks. dishes pictured in the thread are 15 days from inoculation of grain. 10 days after inoculation the transfer was put to agar and it grew out for 5 days.
I posted this in that thread too:
When you pull colonized grains to agar you'll get much faster growth due to the grain nutes you're bringing along. If you do a transfer to just agar after that they'll slow down considerably.
But I like the syringe => grain => agar route for germination and culture isolation - see https://www.shroomery.org/forums/showflat.php/Number/26369976/fpart/all/vc/1 for a whole PE isolation grow log using this method.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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