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Offlinetroy_white42
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Registered: 12/04/20
Posts: 150
Last seen: 2 years, 10 days
Swapping from gourmet
    #27517986 - 10/25/21 07:17 PM (2 years, 6 months ago)

I can grow gourmet pretty legit.  12×12×15 humidity chamber room with a 50g drum pumping humidity with a HOH strip.  Positive/negative airflow for FAE with a fan in/fan out set to auto cycle with a timer and a humidity detector to auto activate the 50g.

I can rock and roll like 300 bags if I built an autobagger and a 50g drum sterilizer.  Haven't went that big yet due to needing a whole saler to dump product.  Working with 2 pressure cookers and a cement mixer for bags atm which is tedious but.. to the point.

I've got about 10 monotubs cut and filtered to do some tests with magic mushrooms.  Also plan on doing some bags and top fruiting the long side of the bag.  I'm guessing the surface area of the mono is preferable for max yield.  I wonder if the size would be bigger with bags just less mushrooms.  Seems a bit easier to avoid contamns..

I'm about 4 agar transfers from prints of several species in.  Got a few good looking rhizomorph plates I'm pushing to grain.  Still working a few more plates to clean contam and get more rhizo.  I have trouble xfering agar to grain as my flowhood is 12×12x12.  LC to grain bags would be optimal then dump in a mono.

I'm wondering if I can upgrade from verm/choir with bags to a poo. If so will the home depot manure work?

Once I get all my 20 different strains on clean agar I plan on crossing them for results as a side project.

Can I fruit the monk in the chamber?  Or just wait till they start to fruit then place them in as adolescents.  Lastly foes anyone have a visual chart on cob web, etc, to ID contams.  I known trich, yeast, mold and when xfering plates rhizo is the better cut to xfer from?

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InvisibleCreonAntigone
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Registered: 05/30/21
Posts: 2,970
Re: Swapping from gourmet [Re: troy_white42]
    #27518568 - 10/26/21 09:14 AM (2 years, 6 months ago)

Why are you developing 20 strains? You aren't even sure yet if you have one strain that's worth it.

Treat your starting process as an alkaloid discovery process. You do not yet know which mushrooms will perform if you're starting multi-spore. Eventually you're going to want to figure out which of the 20 strains grows the most potent mushrooms.. then clone that. Remember that besides APE and a few other studied strains, strains are just names invented by vendors. You ideally want your own strain that has been proven to make mass alkaloids.

Growing a lot of psilocybin is 100% about your culture so you're going to have to do some cultural discovery.

Quote:

several species




I assume you mean several strains? Several species would be if you worked with P. Cubensis, P. Mexicana and P. Tampanensis seperately. But if they're all Cubensis that's one species.


Edited by CreonAntigone (10/26/21 09:16 AM)

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Offlinetroy_white42
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Registered: 12/04/20
Posts: 150
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Re: Swapping from gourmet [Re: CreonAntigone]
    #27518632 - 10/26/21 10:04 AM (2 years, 6 months ago)

Several species and Several strains.  I'm working them all from agar to see which ones I can get the best clean rhizo plates to start focusing on first while I continue to clean up the other ones.  Right now I got some pretty nice clean rhizo plates, a few clean plates that are growing nice but the rhizo isn't as rope.  Gonna xfer this a few more times before I move them to grain.  Gonna clone the rhizo plates and move a few to grain and a few LC jars.  Playing with all options to get a feel for each of them.

I did the same with gourmet until I found the producers on sub.  Retired the semi producers to agar and focused on the big boys which turned out to be blue oyster. Unfortunately, hurricane killed my power and my mini fridge culture's went semi sour so I'm transferring to plates for clean up again.  Life's a struggle. I enjoy the lab work though.

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InvisibleCreonAntigone
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Registered: 05/30/21
Posts: 2,970
Re: Swapping from gourmet [Re: troy_white42]
    #27519511 - 10/26/21 09:49 PM (2 years, 6 months ago)

Quote:

troy_white42 said:
Several species and Several strains.  I'm working them all from agar to see which ones I can get the best clean rhizo plates to start focusing on first while I continue to clean up the other ones.  Right now I got some pretty nice clean rhizo plates, a few clean plates that are growing nice but the rhizo isn't as rope.  Gonna xfer this a few more times before I move them to grain.  Gonna clone the rhizo plates and move a few to grain and a few LC jars.  Playing with all options to get a feel for each of them.

I did the same with gourmet until I found the producers on sub.  Retired the semi producers to agar and focused on the big boys which turned out to be blue oyster. Unfortunately, hurricane killed my power and my mini fridge culture's went semi sour so I'm transferring to plates for clean up again.  Life's a struggle. I enjoy the lab work though.




Well here is your problem. You are judging based on what it looks like on agar and also based on its capacity to produce mushrooms. But you don't want to produce just any mushrooms, you want to produce potent mushrooms - the very strain that might make the cleanest-looking growth may produce fruits that are hundreds of times lower in alkaloidal potency due to the genetic lottery.

There are two ways of testing a strain of magic mushrooms for alkaloidal potency. One is to use lab equipment to isolate the alkaloids; the other is to use one's mouth and eat it. Eating it is the only way possible to test. How else will you know if this shroom you're holding isn't impotent bunk?

What I'd suggest to you: start grabbing transfers from plates as fast as possible, get some actual fungi in front of you, consume said fungi, then you will know which strains are your real producers. Or if you wish to abstain from it, have a friend who is willing to test.

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InvisibleUnknownfungi
Amateur mycologist
I'm a teapot


Registered: 09/28/21
Posts: 584
Re: Swapping from gourmet [Re: CreonAntigone]
    #27520091 - 10/27/21 11:39 AM (2 years, 6 months ago)

You can also test potency with $15 Miraculix psilocybin tests.


--------------------
Gymnopilus luteofolius potency results
Gymnopilus luteus potency results
Active Pluteus potency results
Panaeolus cinctulus potency results
Psilocybe caerulipes potency results
Gymnopilus luteus
Gymnopilus luteofolius
Psilocybe ovoideocystidiata
Psilocybe caerulipes
Panaeolus cinctulus
Pluteus americanus

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