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OfflineFailboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
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Re: Post Your Cultivation Picture of The Day [Re: multifractal]
    #27514061 - 10/22/21 06:16 PM (2 years, 5 months ago)

I got trust issues:shrug:

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Invisiblemultifractal
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Re: Post Your Cultivation Picture of The Day [Re: Failboat]
    #27514066 - 10/22/21 06:19 PM (2 years, 5 months ago)

Haha as do I, which is why I checked this out

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InvisibleStipe-n CapMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: Failboat] * 1
    #27514075 - 10/22/21 06:21 PM (2 years, 5 months ago)

Any plates that I've run that looks anything close to that has failed miserably, without exception.

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Invisiblesandman420
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Registered: 06/17/04
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Re: Post Your Cultivation Picture of The Day [Re: multifractal]
    #27514080 - 10/22/21 06:22 PM (2 years, 5 months ago)

Quote:

multifractal said:
My PEU×MM F2 plates are pretty uniform by now (my F3 plates are another story) but I see multiple types of mycelium on the substrates, one that looks sort of like a dry gray mold that never beads with moisture but is indeed mycelium and several more rhizomorphic tufts that pin later on.

Im interested to hear if you see the same type of grayish mycelium that I mentioned.

Speaking of sectoring, check out this one F3 plate (ostensibly the worst of the lot):



I checked this one out under a microscope I managed to get access to (had to call in a small favor) and saw no signs of anything besides cubensis mycelium to my relatively untrained eye that is so I could be mistaken of course. I get to use it again on Monday so I'll try taking some photographs through the lens.




I believe (with little to nothing to back it up) this to be from a mycovirus. It's literally dying on the edges. I have a similar plate that I suspect has a mycovirus. It grew slowly for a bit and then just died over 2 months ago and has just been sitting, dead. Was from a melmac spore swab that I made myself :frown:

Any flaky stuff is nutritional yeast added to the agar, shit doesn't dissolve very well.













--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineFailboat
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Re: Post Your Cultivation Picture of The Day [Re: sandman420]
    #27514082 - 10/22/21 06:23 PM (2 years, 5 months ago)

I've got this with some APE plates

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InvisibleStipe-n CapMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: Failboat]
    #27514087 - 10/22/21 06:26 PM (2 years, 5 months ago)

Melmak and APE have been the culprits for my experience as well, especially mak swabs.

Lab grade yeast dissolves quite well.

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Invisiblesandman420
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Re: Post Your Cultivation Picture of The Day [Re: Failboat]
    #27514089 - 10/22/21 06:26 PM (2 years, 5 months ago)

did it dies and stop growing?

also it could make sense for a mycovirus to attack a couple of strains that rely on swabbing only from the way we trade spores. Could be something to think about.

Quote:

p9hu7 said:

Lab grade yeast dissolves quite well.





But then what would I do with this 2lb jug of nutritional yeast I bought 15 years ago?!


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (10/22/21 06:35 PM)

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Invisiblemultifractal
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Re: Post Your Cultivation Picture of The Day [Re: sandman420]
    #27514099 - 10/22/21 06:34 PM (2 years, 5 months ago)


Some time later:

(Ended up pinning like crazy with nice fat fruits)
Cleaned up well:


(Melmak)

I had it with these plates and fruited successfully with them on bulk substrate so at least one exception. I think it's just competing colonies that don't get along with each other in that case. Could be other causes of it though leading to difficulties that some report.

Edited by multifractal (10/22/21 06:48 PM)

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Offlinenektar61S
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Re: Post Your Cultivation Picture of The Day [Re: sandman420]
    #27514119 - 10/22/21 06:43 PM (2 years, 5 months ago)

Quote:

sandman420 said:

But then what would I do with this 2lb jug of nutritional yeast I bought 15 years ago?!




You've been on here way longer than me, but I'll throw in my 2 cents since you're asking.

If you're worried it has bacteria, put it in jars with the lid on loose and PC for 2 hours before use.

Not sure about viruses. I've only heard in theory of viruses on shrooms and mycelium. I think it's possible,  but I've read thousands of posts on here and never seen it.

Try the advanced search. Limit it to last 5 years, most of what's on here from before that is old tek, and the further you go back the more it's conjecture and the less it's truth. (with a few exceptions).
https://www.shroomery.org/forums/search.php

with advanced search I also recommend ticking
" Only show one result per thread"
or you'll get too much info to sort easily.


--------------------
-NEW? Start here.

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Invisiblesandman420
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Re: Post Your Cultivation Picture of The Day [Re: nektar61] * 1
    #27514131 - 10/22/21 06:51 PM (2 years, 5 months ago)

Oh no I was joking about him saying a different type of yeast dissolves better, added to agar for benefit not a contam.

Mycoviruses are totally a real thing not theory. A cool one is Called Mushroom Virus X


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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InvisibleStipe-n CapMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: sandman420]
    #27514141 - 10/22/21 06:57 PM (2 years, 5 months ago)

I bought yeast to see if it would coax some cultures into expressing different morphology, I was disappointed to observe no effect....it actually caused one of my most rhizomorphic cultures to lose its rope.

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Offlinenektar61S
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Re: Post Your Cultivation Picture of The Day [Re: sandman420]
    #27514147 - 10/22/21 06:58 PM (2 years, 5 months ago)

Quote:

sandman420 said:

Mycoviruses are totally a real thing not theory. A cool one is Called Mushroom Virus X




I'm almost afraid to look. ha ha.


--------------------
-NEW? Start here.

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InvisiblefahtsterM
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Re: Post Your Cultivation Picture of The Day [Re: multifractal] * 1
    #27514180 - 10/22/21 07:21 PM (2 years, 5 months ago)

Quote:

multifractal said:

Im interested to hear if you see the same type of grayish mycelium that I mentioned.





I do get a lot of what you describe that kind of stalls out.. happens almost every time with my early gens.  This is PE/pfrs f2


When that area stalls out, I have to wait til aggressive myc to come and colonize those parts which makes the cakes take forever to fully colonize.  Those are from a jar print so I’m pretty certain they’re not bacterial.. at least excessively… t he same SS was used to knock up a glc jar that went to qts and looks great.  I think some sub strains are just shit genes

I’ve got some of that crazy aggressive nat myc in a couple cakes and it’s actually saving my ass because it’s taking over the areas that stalled and pretty sure these jars are bacterial.. came from a foil print.. I just need a fruit to clone so I can get a tub goin


Faht

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Invisiblemultifractal
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Re: Post Your Cultivation Picture of The Day [Re: fahtster]
    #27514201 - 10/22/21 07:37 PM (2 years, 5 months ago)

Ah yes, very similar to how it sometimes looks on grain. Here is a shot of it on substrate:




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InvisibleThe Fresh PrintsS
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Re: Post Your Cultivation Picture of The Day [Re: multifractal] * 3
    #27514252 - 10/22/21 08:10 PM (2 years, 5 months ago)



Wheat/Milo


Got my brew gear out of storage. Game changer for grain prep.


--------------------

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OfflineSmoothcat
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Registered: 10/07/15
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Re: Post Your Cultivation Picture of The Day [Re: The Fresh Prints] * 1
    #27514733 - 10/23/21 07:57 AM (2 years, 5 months ago)

PESA spawn bag nearly at 100%



Always be bagging :thumbup:


--------------------
Back once again with the ill behaviour



Links I like

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Invisiblesandman420
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Re: Post Your Cultivation Picture of The Day [Re: The Fresh Prints]
    #27514770 - 10/23/21 08:34 AM (2 years, 5 months ago)

Quote:

The Fresh Prints said:


Wheat/Milo


Got my brew gear out of storage. Game changer for grain prep.




nice! I guess there is some sort of mesh to prevent the spigot from clogging up too or does the false bottom keep it all out?

do you wear a witches hat and cackle while you toil over it on occasion?


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineB H O
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Re: Post Your Cultivation Picture of The Day [Re: sandman420]
    #27514775 - 10/23/21 08:40 AM (2 years, 5 months ago)

Does anyone here have any experience with enigma?



I received a few edible liquid cultures from a friend a while back and was told the one labeled lions mane was actually enigma. I put it to grain and then fruited a little shoebox. It started pinning about 3 weeks ago and it stalled but didn’t look aborted so I left it alone. Eventually I forgot about it and found it this morning and that’s what I found. I see lots of the pins turned into fan like shapes and shows a lot of blue bruising so it looks like it’s psychoactive..

But here in the middle… is this normal for an Enigma cluster or is this literally some lions mane mixed with Enigma?

I’m so confused…

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Offlinerockyfungus
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Re: Post Your Cultivation Picture of The Day [Re: B H O]
    #27514785 - 10/23/21 08:58 AM (2 years, 5 months ago)

WTF, isn't that a lions mane? Has the classic pom just not very toothy from the mono I'd assume. Is it fuzzy or a bunch of tendrils?
Really looks like enigma fins and a big bear's head, nice sized too.

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OfflineA.k.aM
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Re: Post Your Cultivation Picture of The Day [Re: rockyfungus] * 1
    #27514791 - 10/23/21 09:13 AM (2 years, 5 months ago)

Lol yeah I think they must be mixed. Or maybe if he refilled the lions mane syringe with enigma some myc survived.


It does look a little off though. Eat some and see if you trip :lol:  They’re supposed to be synergistic anyways.


--------------------
LAGM2020

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