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Offlineferret
strange

Registered: 08/20/01
Posts: 164
Loc: VIC, Australia
Last seen: 2 years, 2 months
'subaeruginosa' - brown AND purplebrown spores
    #2751317 - 06/01/04 12:10 AM (13 years, 4 months ago)

i found two patches of subs, one with adnate gills the other adnexed gills, consistently. its a shitty pic but you can see the adnate gilled subs definately have a plain brown sporeprint, the ascending gilled subs have the purple-brown sporeprint.

  :confused: pretty fucked up.


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Invisiblezee_werp
a fractalcreature
 User Gallery

Registered: 03/25/03
Posts: 1,026
Loc: Aotearoa
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: ferret]
    #2751352 - 06/01/04 12:19 AM (13 years, 4 months ago)

Well, given the huge amount of variation within the subaeruginosa species itself, and especially since they were from 2 seperate patches, its not that surprising to see some difference in spore colour.

Of course, without microscopically identifying them, you have no way of knowing for sure if both of them are infact Psilocybe subaeruginosa.

But yes it is definatley possible and probably quite common for variations in sub-species to have different spore colours.

Nice find by the way!


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OfflineHardicus
MR
Male
Registered: 06/27/03
Posts: 68
Loc: Australia
Last seen: 6 months, 14 days
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: ferret]
    #2751947 - 06/01/04 03:20 AM (13 years, 4 months ago)

That's interesting, i've found many macroscopically different p.subs, but no matter how extremely different looking and where they came from, the sporeprints have always been identical.

Very interesting.


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Offlinesuboriginal
enthusiast

Registered: 05/06/04
Posts: 278
Last seen: 13 years, 19 days
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: Hardicus]
    #2752095 - 06/01/04 04:46 AM (13 years, 4 months ago)

I made a bunch of prints from the same batch/ patch recently, cause I was starting to think a 'purple' tint from sub's was like the emperors new clothes... I can never see it on paper prints (even white paper in great light etc...), and this time made some on paper, some on foil and some on brown cardboard, assigned at random all from the same patch. No purple in the paper, but most of the ones I made on foil it was pretty obvious to me. I'll attach some decent pics showing the proper contrast later. Meantime here's an old batch paper-print I heve on the server already, and a crappy rez pic of one of the foil prints from the last batch/ patch.





--------------------
Peace, love and organic brown rice...


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Offlineferret
strange

Registered: 08/20/01
Posts: 164
Loc: VIC, Australia
Last seen: 2 years, 2 months
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: suboriginal]
    #2752169 - 06/01/04 06:03 AM (13 years, 4 months ago)

hmm and my last batch did it too... umbonate/adnate = brown
wavy/ascending = purple.

what colour are your nz sub prints zee?

ive had a few quick goes at viewing some fresh gill fragments under light microscope, but im shit at taking thin sections off the gills. anyone got any tips to get a nice thin slice?


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Offlinesuboriginal
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Registered: 05/06/04
Posts: 278
Last seen: 13 years, 19 days
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: ferret]
    #2752186 - 06/01/04 06:29 AM (13 years, 4 months ago)

Don't do it while they're at room temp, chill them first (don't freeeze' em though, unless you want to make a 'cryosection'... very thin indeed, but distorted cells!). I chill the scalpel blade/ razor in packet alongside them, I prefer to use an actual scalpel with a handle, to minimise heat tranferance to the shroom flesh from a directly gripped razor blade, but this might be over-kill. Not even sure chilling the blade helps that much, but I've gotten some great thin sections this way.


--------------------
Peace, love and organic brown rice...


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Offlinesuboriginal
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Registered: 05/06/04
Posts: 278
Last seen: 13 years, 19 days
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: suboriginal]
    #2755901 - 06/02/04 07:03 AM (13 years, 4 months ago)

The 'better' pics of spore prints from the same patch of Melbourne subaeruginosa... purple only evident on foil...







--------------------
Peace, love and organic brown rice...


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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: 'subaeruginosa' - brown AND purplebrown spores [Re: ferret]
    #2755912 - 06/02/04 07:19 AM (13 years, 4 months ago)

I know Ferret and he knows his subs...
Teh colour is weird - we better get those under a microscope and have a look see...
The confirmed eucalypta variant from NSW had a very brownish print and as you ahev seen, very adnate attachment whereas the wavys and other vic subs had the strong ascending attachment with purple gills...
interesting... Teh thing is though that those subs around at your palce were all drying like vic subs and not like those eucys - but since they are all possibily expressions of the same entity possibly in the process of delineating then who knows..


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