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mushboy
modboy
Registered: 04/24/05
Posts: 32,654
Loc: where?
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hobotechnology]
#27505919 - 10/15/21 07:58 PM (2 years, 5 months ago) |
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noice lc
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AtmozFear
just a shade of myself
Registered: 01/25/19
Posts: 1,032
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Re: AGAR ENVY! (Anything and All things agar!) [Re: karri0n]
#27506066 - 10/15/21 11:02 PM (2 years, 5 months ago) |
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Quote:
karri0n said: I've been holding onto these for a bit. I wanted to make sure it was viable before posting it.
Blackstrap Molasses.
Blackstrap molasses has about half the sugar of LME and approximately 1000% of the various micronutrients, namely calcium, iron, copper, potassium, magnesium, manganese, selenium and vitamin B6. Many of these minerals are dissolved as salts, making them more absorbable than say, in the form of rock gypsum, dolomite, or even the cellulose filled hulls of grain.
Blackstrap molasses agar 8g blackstrap molasses 10g agar 500ml Brita filter water
The plate pin has mold on the plate - sterile technique problems on my part. However I like the sectoring happening on the one plate.
The gourmets love this stuff. Chicken of the woods and ganoderma Tsugae.
I also did a couple of LC using 1.5g BSM to 500ml filtered water. I would do 1g or .5g next time. It's obviously dark in color, but the clarity is better than any LME broth or grainwater I have ever made. No sediment whatsoever, and I think it's because the BSM starts as a liquid?
PF redspore on the left. It's in a jar with a filter I normally wouldn't use for LC, so I am afraid to swirl it too hard. APE on the right, broken up nicely.
I would love to see someone experiment with this stuff who has a hood and real plates - the condiment cups are ok but you can't get the full picture.
Thanks so much for this! My LME became moldy and I thought I had nothing but brf left, but you made me realize I could use the BSM that I use in compost tea for my plants!
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nektar61
Into SporePlay
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AtmozFear]
#27507137 - 10/16/21 08:03 PM (2 years, 5 months ago) |
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Both are 13 days old.
Psilo Nat:
Psilo cyan
-------------------- -NEW? Start here.
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Wall.E
Bacteria's Bitch
Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 2
#27507181 - 10/16/21 08:45 PM (2 years, 5 months ago) |
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Nats looking good
-------------------- Lifeβs shit, but Iβm loving it
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multifractal
Student
Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E] 2
#27507287 - 10/16/21 10:40 PM (2 years, 5 months ago) |
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Melmak.
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A.k.a
Stranger
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
#27507561 - 10/17/21 07:35 AM (2 years, 5 months ago) |
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Yeah that nat culture should be good. Thatβs exactly the type of myc I try to find. Not super rhizo and doesnβt immediately climb the walls.
-------------------- LAGM2020
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Samushlove
Water
Registered: 03/09/21
Posts: 248
Loc: Foothills
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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a] 1
#27508047 - 10/17/21 02:37 PM (2 years, 5 months ago) |
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PE/B+ plate has been making pins for months...
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Baba Yaga
Fomo Kills The Cat
Registered: 09/13/20
Posts: 4,153
Loc: Try 'n' Error Boot Camp
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Samushlove] 1
#27508115 - 10/17/21 03:24 PM (2 years, 5 months ago) |
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Bacterial Pan cambo clone pinning directly from clone tissue
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multifractal
Student
Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Baba Yaga] 4
#27508178 - 10/17/21 04:17 PM (2 years, 5 months ago) |
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More progress on the culture made from spores I took from wild, pasture cubensis. This plates cousins went to grain yesterday.
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Failboat
Fuck Up
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
#27508257 - 10/17/21 05:39 PM (2 years, 5 months ago) |
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Nichrome
I'm a torso!
Registered: 12/17/18
Posts: 6,764
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
#27508464 - 10/17/21 08:47 PM (2 years, 5 months ago) |
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Quote:
multifractal said:
More progress on the culture made from spores I took from wild, pasture cubensis. This plates cousins went to grain yesterday.
I love to see the wild beasties.
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βTea is the magic key to the vault where my brain is kept.β β Frances Hardinge
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multifractal
Student
Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] 2
#27508512 - 10/17/21 09:26 PM (2 years, 5 months ago) |
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Me while doing SAB work:
You can't show any fear when transferring this culture or it will mount you in a second flat and take its pleasure. Prints available soon.
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Baba Yaga
Fomo Kills The Cat
Registered: 09/13/20
Posts: 4,153
Loc: Try 'n' Error Boot Camp
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
#27508521 - 10/17/21 09:35 PM (2 years, 5 months ago) |
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Looks legit , just make sure your arm holes are big enough.
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nektar61
Into SporePlay
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
#27508543 - 10/17/21 10:02 PM (2 years, 5 months ago) |
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Quote:
multifractal said: You can't show any fear when transferring this culture or it will mount you in a second flat and take its pleasure. Prints available soon.
Sounds like P-Nat mycelium.
-------------------- -NEW? Start here.
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aNewBeginning
Registered: 06/26/21
Posts: 708
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 2
#27508621 - 10/18/21 01:22 AM (2 years, 5 months ago) |
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Transferred my previously shown Ps. cyan plate to PDA (+yeast) and to LMEA (+yeast).
Ps. Cyan T4 - PDA
Ps. Cyan T4 - LMEA
T1 Ovoid (from print)
TAT Swab T1
The T0 plate got contaminated but the swab was fine so I transferred the swab to a new plate.
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Doctor Mario
πππππ
Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning] 2
#27509548 - 10/18/21 08:57 PM (2 years, 5 months ago) |
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This plate is heavily contaminated but I wanted to try to clone this pin anyway.
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conehead
Stranger
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario] 3
#27509561 - 10/18/21 09:14 PM (2 years, 5 months ago) |
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First plate I'm proud enough to post here haha. T2 of a clone I took out of my first shoebox. Sent to millet last night!
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narmaduke
Stranger
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Re: AGAR ENVY! (Anything and All things agar!) [Re: conehead]
#27509631 - 10/18/21 10:25 PM (2 years, 5 months ago) |
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Are any of these plates salvageable? They come from a super old GT MS syringe
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multifractal
Student
Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
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Re: AGAR ENVY! (Anything and All things agar!) [Re: narmaduke]
#27509641 - 10/18/21 10:41 PM (2 years, 5 months ago) |
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You sometimes see growth like this on spore syringe plates. I'd go for the 8 o'clock area of the second plate or some near the bottom of the small circular spot below the center of the third plate and evaluate those transfers to decide if you want to go forward with this culture.
Quote:
conehead said: First plate I'm proud enough to post here haha. T2 of a clone I took out of my first shoebox. Sent to millet last night!
This growth pattern was caused by the transfer wedge landing on a small puddle of surface moisture:
Edited by multifractal (10/18/21 11:30 PM)
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Atomsplit
Registered: 01/16/21
Posts: 1,511
Loc: SAB
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] 2
#27509664 - 10/18/21 11:28 PM (2 years, 5 months ago) |
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You all are killing it!
T0C. T-4 was about to grain it but haven't been sleeping well so maybe when I feel rested.
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