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OfflineJXAllen
Cannalyst


Registered: 08/06/21
Posts: 615
Last seen: 14 hours, 1 minute
Re: Cultivation General Discussion [Re: Biskop] * 2
    #27495658 - 10/07/21 06:04 AM (2 years, 4 months ago)

Quote:

Biskop said:
Yo @JXAllen you bastard! You make me wish I started with PF Tek. I’ve only harvested one flush so far and everything else contaminated. Those mushies look real nice. I wouldn’t even complain, but yeah it’d be good to know if it’s just genetics or something you’re doing wrong.

Didn’t see you post for a while I thought you were tripping haha. Good work!




Ha Ha! Thanks man! I'm definitely liking the simplicity and ease of the PF Tek. I've got two FCs running right now, both with 5 cakes each. The first five have all given me their first flush and two just finished their second. The second tub is a couple weeks behind the first and is just starting to pin. If you decide to give it a try and I highly suggest you do, here are a couple lessons I've learned.

Go for the wide mouth 8oz canning jars, not the tall regular mouth ones. You'd think 8oz is 8oz, but the shorter, wider jars colonize faster and are less prone to stalling. That goes for trying out larger containers. You increase the rist of competitors and stalling.

Make sure you use course vermiculite!! If you use fine, the cake will collapse in the PC and the verm in the filter layer will fall down the sides. This makes the verm filter layer all but useless.

I've had a couple neat looking cakes in my first flush. Here's my KSSS cake. Very neat looking! like an alien or a monster. I think it looks like a beholder from dungeons and dragons :laugh:



My unknown mushroom turned out to be a pretty looking leucistic


And here's the second flush from my Mazatapec cake.



I've got the first flush from each cake in their own jars with a silica desiccant. They were all dried for 12-18 hours in a dehydrator before jarring. Cracker dry, I got just over 16g!! Just over half an ounce from my first flush from the cakes. Since this is all for personal consumption and sharing with friends, this is a great result for the effort!


I've also got the 6 millet jars colonizing from the agar wedges I added a week and a half ago. I shook them 5 days ago, they're coming along nicely! I added pictures to my thread. They should be ready to go into shoe boxes in a week or so.


--------------------
There are only 10 types of people in the world, those that understand binary and those that do not.
Cultures I'm workin on

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OfflineBiskop
Fokken Prawns
Male


Registered: 12/09/17
Posts: 192
Loc: Vietnam 🇻🇳
Last seen: 1 year, 8 months
Re: Cultivation General Discussion [Re: JXAllen]
    #27495839 - 10/07/21 09:43 AM (2 years, 4 months ago)

I’m honestly impressed! Most beginners like me don’t know what they’re doing and they fuck their first grows. You’ve obviously done your research and it has payed off.

Damn I should’ve gone with PF that's a really good haul for such a tiny jar. I’ll get there one day!

Cheers for the tips and good luck with them big as grain jars!

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OfflineOra
Stranger
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Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 2 months
Re: Cultivation General Discussion [Re: fahtster]
    #27495929 - 10/07/21 11:12 AM (2 years, 4 months ago)

Quote:

fahtster said:
Are you misting them?  They look a bit wet.  That yellow fuzz is suspect for sure




It was 2nd or 3rd flush and I did dunk the sub under water for a bit too long. The centers of the stem did look very dark/saturated with water.

You think its safe to eat?

Also to note the dunked water when dumped was yellowish

Edited by Ora (10/07/21 11:13 AM)

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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,884
Last seen: 13 hours, 42 minutes
Re: Cultivation General Discussion [Re: Ora]
    #27496061 - 10/07/21 12:57 PM (2 years, 4 months ago)

AKA: in another post we talked about cloning or taking tissue,
Baba Yaga, we also discussed this.


Before when I tried cloning, I didn't clean or treat the fruits at all, and could not get good enough growth to isolate


After adding the step of dipping tissue/fruits in 70% iso, less than 10 seconds, I was able to save a TWC clone, and establis a natalensis clone.

When recently attempting to grow myc, thinking on recent discussion, I dipped the 3 subtropica fruits for a longer time.


The fruits ended up total black, and nothing grew from them


What's the sweet spot here?
Is the black fruit a fluke?
I saw Aka post that he got growth from blackened fruits

What's the dealio here compadres


--------------------
Willpower is the one true virtue


Edited by smalltalk_canceled (10/07/21 12:59 PM)

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Offlinesilosound

Registered: 03/04/21
Posts: 135
Loc: CA
Last seen: 1 year, 4 months
Re: Cultivation General Discussion [Re: Ora]
    #27496103 - 10/07/21 01:36 PM (2 years, 4 months ago)

I've got a couple boxes of APEs going, my first time with them. I have a similar thing happening with the yellowing on the second flush. Like you, I think I got the substrate oversaturated after the first flush. I kept hearing, "APEs like water" and I guess I took it too far.

The fruits are really wet and some of the myc is yellowish at the base and even some of my stipes aren't pure white. My theory is that the color from the coir is leaching into the fruits/myc because it's so wet. Nothing else seems off, I'm pretty sure I don't have any contams and the fruits look fine when cut, aside from the color and wetness :shrug:

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Invisiblemilkboy
Child
I'm a teapot User Gallery


Registered: 05/01/21
Posts: 2,318
Re: Cultivation General Discussion [Re: silosound]
    #27496130 - 10/07/21 01:52 PM (2 years, 4 months ago)

Speaking of APE,
Swabbed these 7 guys today, 2 qtips each both ends so 4 swabs from each one. Wasnt really sure when was the best time to swab so I grabbed a variety of caps in shotgun approach.


--------------------
Gummies by milkboy
Milkboys Public Display of Affection

"Vires acquirit eundo"


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InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,343
Trusted Cultivator
Re: Cultivation General Discussion [Re: milkboy]
    #27496230 - 10/07/21 02:58 PM (2 years, 4 months ago)

I just wait until the caps are opened up as much as possible so you can get those swabs in there easily

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InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,293
Trusted Cultivator
OG Cultivator
Re: Cultivation General Discussion [Re: smalltalk_canceled] * 1
    #27496246 - 10/07/21 03:16 PM (2 years, 4 months ago)

Quote:

smalltalk_canceled said:
AKA: in another post we talked about cloning or taking tissue,
Baba Yaga, we also discussed this.


Before when I tried cloning, I didn't clean or treat the fruits at all, and could not get good enough growth to isolate


After adding the step of dipping tissue/fruits in 70% iso, less than 10 seconds, I was able to save a TWC clone, and establis a natalensis clone.

When recently attempting to grow myc, thinking on recent discussion, I dipped the 3 subtropica fruits for a longer time.


The fruits ended up total black, and nothing grew from them


What's the sweet spot here?
Is the black fruit a fluke?
I saw Aka post that he got growth from blackened fruits

What's the dealio here compadres




Have you tried H2o2 instead of iso?  I know H2o2 works.. iso would give me pause

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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 4,091
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Cultivation General Discussion [Re: fahtster] * 2
    #27496296 - 10/07/21 03:41 PM (2 years, 4 months ago)

I still have to try H2O2 but have used bleach in various concentrations and soaking times with washing in sterile water afterwards,
made the solution way to strong the first couple of times which stunned recovery too much and bacteria showed up before the myc recovered.

On my last run I bathed them in anti bacterial soap solution for 30 min with a few shakes in between. That worked alright which means I still
had bacteria showing up but the myc was recovering fast and grew past the bacteria on half the plates.

When using just a few drops of bleach on 40ml of water and with the soap all my pans blackened but were still recovering fine with first signs
of growth after 24hrs or less.

Here is another tip, if you have lots of bacteria covering the agar around your pin while the fruit itself is showing growth
you can simply transfer the pin or what ever you got to a new plate placing it with the recovery growth side down onto the agar.
That will give the clone enough of an advantage to outrun the contamination.

It looks like I got 8 or 9 pan clones ready in a couple of weeks, just need a few more transfers for good measure.

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Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 2,884
Last seen: 13 hours, 42 minutes
Re: Cultivation General Discussion [Re: Baba Yaga] * 1
    #27496332 - 10/07/21 04:09 PM (2 years, 4 months ago)

Got a few wood chip corn jars coming out

Want to transfer serbica to it, luteo and azur

Then straight to coir

Why ever do straight grain jars with exotics, I ask myself.

See after fruiting alot of supposed wood eater luteo from grains (also agar, but not the point) I'm wondering:

If a wood source is a hard requirement for fruiting supposed "wood eaters", is me easily fruiting luteo without wood a very rare exception, or are there many woodeaters, maybe more than half as a suggested frequency, that can fruit from grains, or corn specificly, when spawned to coir, fully colonized, and meetings the temperature requirements?

Oysters (pink, king, pearl), subtropica, semilanceata*, luteo, all pin from corn, pseudoaztecorum, indoors, but not azurenscens, don't know about serbica

*Spawned outdoors

What if the corn 🌽 the magic corn 🌽 provides lignin or cellulose in some form or acts like it, and that ends up being "close enough"?

One day the unified corn compendium will come.
Also one day find out why outdoors is such a big deal when they are not mycoriza


--------------------
Willpower is the one true virtue


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OfflineFailboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 12 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: fahtster]
    #27496358 - 10/07/21 04:24 PM (2 years, 4 months ago)

Quote:

fahtster said:
Quote:

smalltalk_canceled said:
AKA: in another post we talked about cloning or taking tissue,
Baba Yaga, we also discussed this.


Before when I tried cloning, I didn't clean or treat the fruits at all, and could not get good enough growth to isolate


After adding the step of dipping tissue/fruits in 70% iso, less than 10 seconds, I was able to save a TWC clone, and establis a natalensis clone.

When recently attempting to grow myc, thinking on recent discussion, I dipped the 3 subtropica fruits for a longer time.


The fruits ended up total black, and nothing grew from them


What's the sweet spot here?
Is the black fruit a fluke?
I saw Aka post that he got growth from blackened fruits

What's the dealio here compadres




Have you tried H2o2 instead of iso?  I know H2o2 works.. iso would give me pause



I've done iso and hand sanitizer that eas mainly ethanol with success.

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Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 2,884
Last seen: 13 hours, 42 minutes
Re: Cultivation General Discussion [Re: Failboat]
    #27496375 - 10/07/21 04:37 PM (2 years, 4 months ago)

Also big fuck you to psilocybe azurenscens

This is the the most boring, unresponsive, lazy, greedy under species of psilocybe that I have encountered. It only wants to eat, dry out and die

So fuck you azur

Also back to the flowerpot



They said it's not azurenscens in the woodeater thread but I don't believe them

Look at third picture and tell me that's not a dick

And if it's not azurenscens what the fuck is it


--------------------
Willpower is the one true virtue


Edited by smalltalk_canceled (10/07/21 04:39 PM)

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InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,343
Trusted Cultivator
Re: Cultivation General Discussion [Re: Failboat] * 1
    #27496378 - 10/07/21 04:39 PM (2 years, 4 months ago)

Quote:

Quirkmeister92 said:
Quote:

fahtster said:
Quote:

smalltalk_canceled said:
AKA: in another post we talked about cloning or taking tissue,
Baba Yaga, we also discussed this.


Before when I tried cloning, I didn't clean or treat the fruits at all, and could not get good enough growth to isolate


After adding the step of dipping tissue/fruits in 70% iso, less than 10 seconds, I was able to save a TWC clone, and establis a natalensis clone.

When recently attempting to grow myc, thinking on recent discussion, I dipped the 3 subtropica fruits for a longer time.


The fruits ended up total black, and nothing grew from them


What's the sweet spot here?
Is the black fruit a fluke?
I saw Aka post that he got growth from blackened fruits

What's the dealio here compadres




Have you tried H2o2 instead of iso?  I know H2o2 works.. iso would give me pause



I've done iso and hand sanitizer that eas mainly ethanol with success.



Ya'll sterilized water works perfectly fine. Chemicals damage mycelium. Water doesn't.

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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 4,091
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27496387 - 10/07/21 04:46 PM (2 years, 4 months ago)

Quote:

smalltalk_canceled said:
Also big fuck you to psilocybe azurenscens

This is the the most boring, unresponsive, lazy, greedy under species of psilocybe that I have encountered. It only wants to eat, dry out and die

So fuck you azur

Also back to the flowerpot



They said it's not azurenscens in the woodeater thread but I don't believe them

Look at third picture and tell me that's not a dick

And if it's not azurenscens what the fuck is it





I mean conditions can provoke all sorts of phenotypes but if I would have to guess which psilocybe that is
just from the last two pictures with now background info then I would say that's some wonky Ps. zapotecorum.

Do those fruits bruise at all? Could be something non active as well.....most of them are dicks :shrug:

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Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 2,884
Last seen: 13 hours, 42 minutes
Re: Cultivation General Discussion [Re: Baba Yaga] * 1
    #27496463 - 10/07/21 05:46 PM (2 years, 4 months ago)

This man is a genie, a genius, a scholar, because nobldy else than master Baba yaga pointed it out.

When I think of it (after hearing pseudo) I remember a jar of coriander seedf pseudoaztecorum that I discarded, and this must be it.

Wow Baba


--------------------
Willpower is the one true virtue


Edited by smalltalk_canceled (10/07/21 05:47 PM)

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OfflineFailboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 12 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27496485 - 10/07/21 06:06 PM (2 years, 4 months ago)

I really wanna expand my Azurescens now that you've talked about it. It looks clean AF in the fridge on agar, and I got a dumptruck full of mulch heaped up and a few weeks aged now.

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OfflineOra
Stranger
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Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 2 months
Re: Cultivation General Discussion [Re: silosound]
    #27496564 - 10/07/21 07:11 PM (2 years, 4 months ago)

Quote:

PsySounds said:
I've got a couple boxes of APEs going, my first time with them. I have a similar thing happening with the yellowing on the second flush. Like you, I think I got the substrate oversaturated after the first flush. I kept hearing, "APEs like water" and I guess I took it too far.

The fruits are really wet and some of the myc is yellowish at the base and even some of my stipes aren't pure white. My theory is that the color from the coir is leaching into the fruits/myc because it's so wet. Nothing else seems off, I'm pretty sure I don't have any contams and the fruits look fine when cut, aside from the color and wetness :shrug:




hopefully its just the coir leaching out. the fruits looked like they were cracking on the stems and a bit translucent, so probably the oversaturation.

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InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,293
Trusted Cultivator
OG Cultivator
Re: Cultivation General Discussion [Re: Ora]
    #27496664 - 10/07/21 09:43 PM (2 years, 4 months ago)

Quote:

Munchauzen said:
Quote:

Quirkmeister92 said:
Quote:

fahtster said:
Quote:

smalltalk_canceled said:
AKA: in another post we talked about cloning or taking tissue,
Baba Yaga, we also discussed this.


Before when I tried cloning, I didn't clean or treat the fruits at all, and could not get good enough growth to isolate


After adding the step of dipping tissue/fruits in 70% iso, less than 10 seconds, I was able to save a TWC clone, and establis a natalensis clone.

When recently attempting to grow myc, thinking on recent discussion, I dipped the 3 subtropica fruits for a longer time.


The fruits ended up total black, and nothing grew from them


What's the sweet spot here?
Is the black fruit a fluke?
I saw Aka post that he got growth from blackened fruits

What's the dealio here compadres




Have you tried H2o2 instead of iso?  I know H2o2 works.. iso would give me pause



I've done iso and hand sanitizer that eas mainly ethanol with success.



Ya'll sterilized water works perfectly fine. Chemicals damage mycelium. Water doesn't.




I assumed smalltalk was talking about cleaning up outer stem tissue for cloning his smaller exotics.  Have you used just sterile water for outer tissue before?  I use sterile water for inner tissue but thought more was needed for outer

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InvisibleTedsDead
 User Gallery


Registered: 01/03/17
Posts: 4,999
Trusted Cultivator
Re: Cultivation General Discussion [Re: fahtster]
    #27496752 - 10/07/21 11:50 PM (2 years, 4 months ago)

yeah, azzies are the shit!


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,343
Trusted Cultivator
Re: Cultivation General Discussion [Re: fahtster]
    #27496881 - 10/08/21 03:31 AM (2 years, 4 months ago)

Quote:

fahtster said:
Quote:

Munchauzen said:
Quote:

Quirkmeister92 said:
Quote:

fahtster said:
Have you tried H2o2 instead of iso?  I know H2o2 works.. iso would give me pause



I've done iso and hand sanitizer that eas mainly ethanol with success.



Ya'll sterilized water works perfectly fine. Chemicals damage mycelium. Water doesn't.




I assumed smalltalk was talking about cleaning up outer stem tissue for cloning his smaller exotics.  Have you used just sterile water for outer tissue before?  I use sterile water for inner tissue but thought more was needed for outer



use multiple water baths for dirtier specimens. I've done up to 4 dunks in sterile water jars before dropping wild fruits to plates. it works well. swirl em around good in the first few then just a quick dunk in the last one.

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