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InvisibleLittledii
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LC 4 days lme
    #27470894 - 09/16/21 05:50 PM (2 years, 7 months ago)

4 days lme 1-1.3g to 600ml water. How to judge when it’s done, is it close?


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OfflineSlugster
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Re: LC 4 days lme [Re: Littledii]
    #27472283 - 09/17/21 09:55 PM (2 years, 7 months ago)

Are you trying to use inject it as soon as possible? You can always just use a little bit and let the rest keep on growing. Mine usually takes a couple of weeks to fully consume the sugar and stop growing.

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InvisibleLittledii
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Re: LC 4 days lme [Re: Slugster]
    #27472602 - 09/18/21 05:57 AM (2 years, 7 months ago)

Not in a rush at all, when  stirred the pieces are covering the whole jar decently dence. Thanks I just had no clue if their technically done or not

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OfflineAnaxus
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Re: LC 4 days lme [Re: Littledii]
    #27474301 - 09/19/21 11:20 AM (2 years, 7 months ago)

Very cool, what did you used to inoculate? Agar or spores?

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InvisibleLittledii
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Re: LC 4 days lme [Re: Anaxus]
    #27474851 - 09/19/21 07:19 PM (2 years, 7 months ago)

Agar wedge, from MS it was 3rd transfer the plate was kept to keep an eye out for contamination.

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Offlinewickedglass
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Re: LC 4 days lme [Re: Littledii]
    #27477591 - 09/21/21 10:11 PM (2 years, 7 months ago)

That's some really nice growth going on at that rate.
I always put 3.5% nutrients in my LCs, which would be 21g to 600ml of liquid, your ratio is 0.22% at 1.3g per 600ml. I may have to try using less nutrients in my next LC session as the growth/time seems very similar at your low dose compared to my much higher level.

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Invisiblecoversall
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Re: LC 4 days lme [Re: wickedglass]
    #27481461 - 09/25/21 07:22 AM (2 years, 7 months ago)

That LC has lots of growth left in it:

Mine are pretty full of myc when I use them:




btw, this sort of post is more suited to the standard mush cult forum. nothing super advanced going on here.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

Edited by coversall (09/25/21 07:23 AM)

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InvisibleLittledii
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Re: LC 4 days lme [Re: coversall]
    #27482573 - 09/26/21 09:46 AM (2 years, 7 months ago)

How long does that take to get that dence, I suppose it has to do with how much nutrients are in the solution? My bad I just guessed which one this would go under and the usual I’m wrong lol

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Invisiblecoversall
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Re: LC 4 days lme [Re: Littledii]
    #27483491 - 09/27/21 07:48 AM (2 years, 7 months ago)

Quote:

Littledii said:
How long does that take to get that dence, I suppose it has to do with how much nutrients are in the solution? My bad I just guessed which one this would go under and the usual I’m wrong lol




No stress, in the grand scheme of things posting something to the wrong part of a mushroom forum is pretty unimportant :wink:

My broths are pretty low in nutrients, 1g LME to 500ml water. All of the ones pictured are pan cyan LCs which grow a fair bit faster than cubes imo. Most of those LCs were near complete in a week or so.

I recently run my first batch of cube LC and am just wracking my brains as to how long they took.

Looks like my cube LCs took about two weeks to finish up. Mind you they didn't have a stirrer and the media bottles have unmodified lids. I swirl my LCs once or twice a day. I don't know how much of a time diff there would be between my non-stirrer approach and an LC on a proper stir plate.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

Edited by coversall (09/27/21 07:50 AM)

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InvisibleLittledii
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Re: LC 4 days lme [Re: coversall]
    #27484343 - 09/27/21 08:12 PM (2 years, 7 months ago)

Quote:

coversall said:
Quote:

Littledii said:
How long does that take to get that dence, I suppose it has to do with how much nutrients are in the solution? My bad I just guessed which one this would go under and the usual I’m wrong lol




No stress, in the grand scheme of things posting something to the wrong part of a mushroom forum is pretty unimportant :wink:

My broths are pretty low in nutrients, 1g LME to 500ml water. All of the ones pictured are pan cyan LCs which grow a fair bit faster than cubes imo. Most of those LCs were near complete in a week or so.

I recently run my first batch of cube LC and am just wracking my brains as to how long they took.

Looks like my cube LCs took about two weeks to finish up. Mind you they didn't have a stirrer and the media bottles have unmodified lids. I swirl my LCs once or twice a day. I don't know how much of a time diff there would be between my non-stirrer approach and an LC on a proper stir plate.



Quote:

coversall said:
Quote:

Littledii said:
How long does that take to get that dence, I suppose it has to do with how much nutrients are in the solution? My bad I just guessed which one this would go under and the usual I’m wrong lol




No stress, in the grand scheme of things posting something to the wrong part of a mushroom forum is pretty unimportant :wink:

My broths are pretty low in nutrients, 1g LME to 500ml water. All of the ones pictured are pan cyan LCs which grow a fair bit faster than cubes imo. Most of those LCs were near complete in a week or so.

I recently run my first batch of cube LC and am just wracking my brains as to how long they took.

Looks like my cube LCs took about two weeks to finish up. Mind you they didn't have a stirrer and the media bottles have unmodified lids. I swirl my LCs once or twice a day. I don't know how much of a time diff there would be between my non-stirrer approach and an LC on a proper stir plate.




Great info. Mind you they did get thicker not like the ones you posted, when broken up it clouds the whole jar can’t even see through 🤷‍♂️

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Offlinewickedglass
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Re: LC 4 days lme [Re: Littledii]
    #27500475 - 10/11/21 07:44 AM (2 years, 7 months ago)

Had some time today and just finished inoculating 1:500 solution. Thanks for the tips, first time I've seen this ratio. I feel like I've had my head under a rock all this time. Of course now I see it everywhere.
I'm curious to see how these small sample tubes go. 👍


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Invisiblecoversall
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Re: LC 4 days lme [Re: wickedglass]
    #27500605 - 10/11/21 10:10 AM (2 years, 7 months ago)

Quote:

wickedglass said:
Had some time today and just finished inoculating 1:500 solution. Thanks for the tips, first time I've seen this ratio. I feel like I've had my head under a rock all this time. Of course now I see it everywhere.
I'm curious to see how these small sample tubes go. 👍






I love small batch LCs. I was looking at grabbing some smaller media bottles, but like having the head room to swirl without the broth getting near to the rim.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Invisiblec10h12n2o
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Re: LC 4 days lme [Re: coversall]
    #27501054 - 10/11/21 04:31 PM (2 years, 7 months ago)

Quote:

wickedglass said:
That's some really nice growth going on at that rate.
I always put 3.5% nutrients in my LCs, which would be 21g to 600ml of liquid, your ratio is 0.22% at 1.3g per 600ml. I may have to try using less nutrients in my next LC session as the growth/time seems very similar at your low dose compared to my much higher level.




holy shit thats a lot!

i usually use 1.67g to a whole liter

i like this ratio because i get fast colonization and crystal clear broth when its done colonizing. plus its pretty obvious when it is done colonizing

still gets surprisingly thick

Quote:

coversall said:
Quote:

wickedglass said:
Had some time today and just finished inoculating 1:500 solution. Thanks for the tips, first time I've seen this ratio. I feel like I've had my head under a rock all this time. Of course now I see it everywhere.
I'm curious to see how these small sample tubes go. 👍






I love small batch LCs. I was looking at grabbing some smaller media bottles, but like having the head room to swirl without the broth getting near to the rim.




me too! i usually use a stir bar on a plate though so no need for much headroom

small batch LCs is my favorite way to test cultures, speeds things up so much. LC is typically ready way before the first grain master would be, and  then you can expand it so much faster. i like running test grows from an LC, and then by the time i know if its something i want to work with some more, all i have to do is use that same LC to make more spawn (compared to going back to a slanted or plated culture, or even saving a master in the fridge)


--------------------

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Invisiblecoversall
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Re: LC 4 days lme [Re: c10h12n2o]
    #27501814 - 10/12/21 10:16 AM (2 years, 7 months ago)

Quote:

c10h12n2o said:
Quote:

wickedglass said:
That's some really nice growth going on at that rate.
I always put 3.5% nutrients in my LCs, which would be 21g to 600ml of liquid, your ratio is 0.22% at 1.3g per 600ml. I may have to try using less nutrients in my next LC session as the growth/time seems very similar at your low dose compared to my much higher level.




holy shit thats a lot!

i usually use 1.67g to a whole liter

i like this ratio because i get fast colonization and crystal clear broth when its done colonizing. plus its pretty obvious when it is done colonizing

still gets surprisingly thick

Quote:

coversall said:
Quote:

wickedglass said:
Had some time today and just finished inoculating 1:500 solution. Thanks for the tips, first time I've seen this ratio. I feel like I've had my head under a rock all this time. Of course now I see it everywhere.
I'm curious to see how these small sample tubes go. 👍






I love small batch LCs. I was looking at grabbing some smaller media bottles, but like having the head room to swirl without the broth getting near to the rim.




me too! i usually use a stir bar on a plate though so no need for much headroom

small batch LCs is my favorite way to test cultures, speeds things up so much. LC is typically ready way before the first grain master would be, and  then you can expand it so much faster. i like running test grows from an LC, and then by the time i know if its something i want to work with some more, all i have to do is use that same LC to make more spawn (compared to going back to a slanted or plated culture, or even saving a master in the fridge)




One of the first things I am going to get when I move to a less cramped place is a proper stirring set up.

I haven't tried expanding LCs yet, the idea seems wicked!


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Offlinewickedglass
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Re: LC 4 days lme [Re: coversall]
    #27502472 - 10/12/21 08:43 PM (2 years, 7 months ago)

I put LC culture medium straight into my slants via a 0.22µm filter to make LC to expand from. I've put blue dye in my agar in those pics, it's obviously better to not dye the agar if you want to more easily see your mycelium growing out, because it'll leach into your solution 😄




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Offlineyas champ
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Re: LC 4 days lme [Re: wickedglass]
    #27538867 - 11/11/21 07:11 AM (2 years, 6 months ago)

Hello everyone,
what do you mean by LME? honey? white sugar?
I would like to do a liquid culture trial of the species "agaricus bisporus" but I really don't know where to start, can someone please help me?

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OfflineTas75
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Re: LC 4 days lme [Re: yas champ]
    #27539724 - 11/11/21 07:06 PM (2 years, 6 months ago)

Quote:

yas champ said:
Hello everyone,
what do you mean by LME? honey? white sugar?
I would like to do a liquid culture trial of the species "agaricus bisporus" but I really don't know where to start, can someone please help me?




LME usually means Light Malt Extract.

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Offlineyas champ
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Re: LC 4 days lme [Re: Tas75]
    #27542693 - 11/14/21 07:03 AM (2 years, 6 months ago)

merci pour la réponse :rire:
avez vous déjà cultivé l’espèce agaricus bisporus ?

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OfflineDERRAYLD
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Re: LC 4 days lme [Re: yas champ]
    #27543715 - 11/14/21 11:51 PM (2 years, 5 months ago)

Quote:

yas champ said:
merci pour la réponse :rire:
avez vous déjà cultivé l’espèce agaricus bisporus ?




https://www.shroomery.org/forums/postlist.php/Board/13

Post a new thread there, it's for growing gourmet mushrooms.

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