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longbow72
Stranger


Registered: 05/26/21
Posts: 365
Last seen: 23 days, 14 hours
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Spore print to liquid culture?
#27469455 - 09/15/21 02:24 PM (3 years, 4 months ago) |
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Ok so I have made around 20 spore prints from a really good strain I like. Is it possible to just scrape a bunch of spores into the LC and go from there? Or do oi need to make a syringe first?
Thanks
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Freefrom


Registered: 08/20/21
Posts: 45
Last seen: 2 years, 6 months
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Re: Spore print to liquid culture? [Re: longbow72]
#27469461 - 09/15/21 02:29 PM (3 years, 4 months ago) |
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I think that’s what syringes are right
-------------------- 💙 “The thief comes only to steal and kill and destroy; I have come that they may have life, and have it to the full.”
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 8 months
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Re: Spore print to liquid culture? [Re: longbow72]
#27469464 - 09/15/21 02:30 PM (3 years, 4 months ago) |
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You can do it but chances of success are very low in my experience. Spores aren't always clean and scraping things into LC provides a lot of opportunities for contamination. Best is to put spores on agar, do at least 1 transfer to see clean growth, then put a small piece of colonized agar into your LC jar using sterile technique.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,788
Loc: A thought
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Re: Spore print to liquid culture? [Re: Freefrom] 1
#27469467 - 09/15/21 02:32 PM (3 years, 4 months ago) |
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No. Don't go spore to LC. It's stupid. Sorry for sounding rude but it is. Spores to agar, agar to LC. Even then it can be fucked up easily. Spores to LC is a fail even if you succeed.
Edit: Got beat to it again.
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I'm a fungal white supremacist.
Edited by Smartattack (09/15/21 02:33 PM)
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Re: Spore print to liquid culture? [Re: Smartattack]
#27469509 - 09/15/21 03:24 PM (3 years, 4 months ago) |
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Quote:
Smartattack said: No. Don't go spore to LC. It's stupid. Sorry for sounding rude but it is. Spores to agar, agar to LC. Even then it can be fucked up easily. Spores to LC is a fail even if you succeed.
Edit: Got beat to it again.
This.
I did spores in Syringes, packaged as good as you can get, to LC for years! 99% failure rate. Spawning grain to monotubs with Liquid culture being the inoculant of the grain jars, ALWAYS ended in GREEN.
I can't tell you how many times I tried this. In hind sight it taught me never to try the same thing expecting different results. After I did Agar wedge > Grain > Mono or shoe boxes, I ALWAYS got mushrooms. Even if it contaminated on the first, second or third flushes.
My goal was to get clean spawn that could flush until the cake was spent. A shriveled up hunk of mycelium barely resembling the huge fluffy cake it originally was. I never got to completely clean spawn though. Looking back I think my agar plates themselves were a source or contamination because after the first inoculation of a plate I would store it in open air with parafilm. Handling it with unclean or semi clean hands. I never wiped down the plates before make new transfers or inoculating a grain jar. At some point I will pick it up again and those are some of my goals; clean culture/spawn.
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Looking for Trichocereus Cacti
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