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Offlineshr00mFer
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Worth transfering or not
    #27469400 - 09/15/21 01:36 PM (3 years, 4 months ago)

Hi. Recently I made some agar and drop some Ecuador spores from print. After few days I spot thin transparent white film on all area except middle where most of the spores are droped.

But few days later I got this what you see on the pictur. Question is should I make a transfer to another agar, from the middle of this one. Is this mycelium (obviously fluffy) or what?

I dont have any print left (it was small one not much visible so..


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OfflineInsultingLizard
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Re: Worth transfering or not [Re: shr00mFer]
    #27469410 - 09/15/21 01:51 PM (3 years, 4 months ago)

I can't quite tell what I'm looking at. Does it look like a cloud when you look at it with your own eyes? It looks like it rises a full 5 mm off the agar.

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Offlineshr00mFer
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Re: Worth transfering or not [Re: InsultingLizard]
    #27469519 - 09/15/21 03:33 PM (3 years, 4 months ago)

Quote:

InsultingLizard said:
I can't quite tell what I'm looking at. Does it look like a cloud when you look at it with your own eyes? It looks like it rises a full 5 mm off the agar.




It looks like a cloud and it raises. But is just centered. Around area look ok, probably will transfer and see how it goes

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OfflineInsultingLizard
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Re: Worth transfering or not [Re: shr00mFer]
    #27469559 - 09/15/21 04:09 PM (3 years, 4 months ago)

That's not mushroom mycelium then. It never looks like that on a germination plate.

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Offlineshr00mFer
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Re: Worth transfering or not [Re: InsultingLizard]
    #27470298 - 09/16/21 08:03 AM (3 years, 3 months ago)

What is it then? If its fluffy, it could look like this?

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OfflineInsultingLizard
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Re: Worth transfering or not [Re: shr00mFer]
    #27470312 - 09/16/21 08:16 AM (3 years, 3 months ago)

I would guess it's mold.

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Offlineshr00mFer
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Re: Worth transfering or not [Re: InsultingLizard]
    #27470670 - 09/16/21 01:49 PM (3 years, 3 months ago)

Of all the things, I think mold be ruled out. Look at the last few posts on Post your cultivation pic of the day. User QM33 posted similar picture...

I'll transfer it anyway and will report the results.

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InvisibleSmartattack
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Re: Worth transfering or not [Re: shr00mFer]
    #27470683 - 09/16/21 02:03 PM (3 years, 3 months ago)

Looks weird but if it doesn't sporulate or grow quickly it could just be turbo tomentose.


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InvisibleQM33
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Re: Worth transfering or not [Re: shr00mFer]
    #27470688 - 09/16/21 02:09 PM (3 years, 3 months ago)

My picture was alot different, mine that I posted in CPotD was thick mycelium, that of a fruitbody, similar to mycogone.

If you had better pictures it would help, esspecially from the top.

If I understand you correctly, there's a film or discoloration everywhere besides where the mycelium is..? My guess is it's bacterial. But because it seemed to pop up over your whole plate you might not have sterilized your agar appropriately... usually it spreads.
Usually you want to store your petris after they're prepped for 2 weeks in a sterile/sanitized container/ziplock.
But the white stuff is mycelium I would say, stressed aerial spore plate mycelium.
Do a few transfers, one from the center, and a couple from the best looking edges, but try to stay away from thay film you were talking about.
And take the tiniest sample you can, literally, if you can see it it's more than enough.


If you need spores I might be able to send you some sometime.

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InvisibleSmartattack
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Re: Worth transfering or not [Re: QM33]
    #27470694 - 09/16/21 02:16 PM (3 years, 3 months ago)

Yeah could just have a bacterial mote around it and nowhere to grow but inward and upward.


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* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.

Edited by Smartattack (09/16/21 02:16 PM)

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Offlineshr00mFer
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Re: Worth transfering or not [Re: Smartattack]
    #27470727 - 09/16/21 02:43 PM (3 years, 3 months ago)

Quote:

Smartattack said:
Yeah could just have a bacterial mote around it and nowhere to grow but inward and upward.




That was what I was thinking but remained speechless somehow:) since its not spreading wide (2 days already almost same circle) probably its fighting bacteria (thin layer that was surounding it) and grows more upwards than its is supposes to. If transfering will take just small piece of center.

I know, should have waited with agar until I see if its contaminated. I rushed it and it was not necessary to do so. I dont have flow hood, just home made SAB and try to maintain sterile conditions as tight as  possible.

QM33, just wanted to compare pics to state that its possible for fluffy mycelium to grow upwards a bit.

Funny thing with these equadors. Two times me and my friend took them and they were mild. Made a mistake, took em third time and went for drinks in the bar near my house. It blew me off with visuals cartoon style, couldnt stand up and went for a piss cause everything was waaay off. I did eventually later but it was not an easy walk:) Strongest trip to this date, others not even close. Dont make same mistake as I did guys.

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