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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Cultivation General Discussion [Re: bagga]
    #27467614 - 09/14/21 03:58 AM (2 years, 5 months ago)

Making 100ml LCs...

How much LME do you recommend per 100ml? I see 2-4% a lot, which would be 2-4g, but then I see much lower numbers or "just a pinch"..


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Being pissed on does not make you a real man.

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InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 4,091
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Cultivation General Discussion [Re: Guerrilla]
    #27467639 - 09/14/21 04:38 AM (2 years, 5 months ago)

I started doing 0.2% after reading the posts below and it works great, will be testing 0.05% next.

Quote:

Mateja said:
That's why it's a good idea to always make a broth with LME and to not add more than ~0.2% i personally make my broths with 0.05%LME and that gives me almost transparent broth which becomes super easy to inspect. I've don't tests with lots of broths ranging from 2% all the way down to 0.05% and I never noticed any noticeable difference in colonization times.





Quote:

Mateja said:
Here's how they grew without stirring:

Quote:

Mateja said:
I made 5 liquid cultures in varying strengths of ELME at 0.1%, 0.2%, 0.4%, 0.8%, and 1.5% strength.

Updates

12 days after inoculation Jan 3
                0.1%                                0.2%                                  0.4%                                0.8%                                1.5%                                                                   

18 days after inoculation Jan 9

25 days after inoculation, Jan 16

31 days after inoculation, Jan 22

38 days after inoculation, Jan 29

43 days days after inoculation Feb 12



Other experiments:
WBS hydration methods
WBS Hydration/Colonization Times
Water absoprtion in BRF cakes




All LC's colonied the surface and pinned on the surface pretty much around the same time. 



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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Cultivation General Discussion [Re: Baba Yaga]
    #27467651 - 09/14/21 05:07 AM (2 years, 5 months ago)

Thanks for the reply and sourcing those posts for me bro, appreciate it. I’ll go with your suggestion of 0.2%


--------------------
Being pissed on does not make you a real man.

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OfflineEnjoil
I'm a teapot


Registered: 10/29/20
Posts: 2,383
Last seen: 12 hours, 3 minutes
Re: Cultivation General Discussion [Re: Baba Yaga]
    #27467844 - 09/14/21 09:51 AM (2 years, 5 months ago)

I would love to see pictures of your LC’s pinning on the surface if you have any.

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OfflineOra
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Registered: 07/09/21
Posts: 734
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Re: Cultivation General Discussion [Re: Enjoil]
    #27467937 - 09/14/21 11:06 AM (2 years, 5 months ago)

If you got metabolites all over the surface, do pins not like to form around those areas? I'm getting lots of side and bottom pins, but surface has very few. I've compressed the substrate down when spawning so I think the only reason would be the surface has all these metabolites and yellow enigma looking things (not enigma, just looks a little similar)

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InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 4,091
Loc: Hyperspace Chicken Coop
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Re: Cultivation General Discussion [Re: Enjoil]
    #27467941 - 09/14/21 11:10 AM (2 years, 5 months ago)

that comment about pinning LC is still part of the quote, never happened to me but I've
seen photos on here somewhere

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InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 4,091
Loc: Hyperspace Chicken Coop
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Re: Cultivation General Discussion [Re: Ora]
    #27467947 - 09/14/21 11:17 AM (2 years, 5 months ago)

Quote:

Ora said:
If you got metabolites all over the surface, do pins not like to form around those areas? I'm getting lots of side and bottom pins, but surface has very few. I've compressed the substrate down when spawning so I think the only reason would be the surface has all these metabolites and yellow enigma looking things (not enigma, just looks a little similar)




Got any photos? Apart from that, I don't think compressing the substrate alone is a magic bullet against side pins.

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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
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Re: Cultivation General Discussion [Re: Baba Yaga] * 1
    #27467979 - 09/14/21 11:53 AM (2 years, 5 months ago)



--------------------

Crack's Tek's and Shit


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OfflineOra
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27468098 - 09/14/21 01:13 PM (2 years, 5 months ago)

I took them out of the shoeboxes and put into a mono. The sides were pinning so much I assumed I'll get a better flush this way than chancing it and waiting for the surface to pin more.


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 5 months, 17 days
Re: Cultivation General Discussion [Re: Baba Yaga]
    #27468177 - 09/14/21 02:25 PM (2 years, 5 months ago)

Quote:

Baba Yaga said:
Quote:

Ora said:
If you got metabolites all over the surface, do pins not like to form around those areas? I'm getting lots of side and bottom pins, but surface has very few. I've compressed the substrate down when spawning so I think the only reason would be the surface has all these metabolites and yellow enigma looking things (not enigma, just looks a little similar)




Got any photos? Apart from that, I don't think compressing the substrate alone is a magic bullet against side pins.




it isn't, impe


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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OfflineOra
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27468192 - 09/14/21 02:33 PM (2 years, 5 months ago)

i dont get too many sidepins usually until the substrate shrinks so the only thing i can think of for why theres sidepins and almost no pins on the surface is the metabolites. i guess well see what happens in a few days. i almost just want to flip the whole substrate upside down and let it pin from the bottom since the bottom already has a few pins.

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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,343
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Re: Cultivation General Discussion [Re: Ora]
    #27468199 - 09/14/21 02:37 PM (2 years, 5 months ago)

IME, theres a good chance a bunch will abort if you flip the cake. Just let them grow from the bottom as is.

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OfflineOra
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Re: Cultivation General Discussion [Re: Munchauzen]
    #27468207 - 09/14/21 02:41 PM (2 years, 5 months ago)

gotcha, thanks!

i just checked other shoeboxes and it does seem i have side pins on those too, just a little less. ive been avoiding liners because im lazy but it may be time... is there really any benefit to compression then? i think i like not compressing it that way at least if sidepins grow they have more room to push out, whereas when compressed the pins just get completely smashed against the walls.

Edited by Ora (09/14/21 05:07 PM)

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OfflineOra
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Re: Cultivation General Discussion [Re: Ora]
    #27468590 - 09/14/21 07:35 PM (2 years, 5 months ago)

huh initial testing from a couple people of my GT and APE say they experienced more of a body high and slight dizzyness from lower doses sub 1g. they said their previous experiences they got more of a "head high" and they didnt have this dizzyness/body high. they dont know anything about varieties its just whatever others gave them.

i capped some APE in size 0 caps at 180mg each and they produce way more of an effect than microdose level haha. i dont have smaller caps and these are too strong. or i just need to cut it with something to dilute it i guess.

anyway whats your guys' experience?

Edited by Ora (09/14/21 07:37 PM)

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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 5 months, 17 days
Re: Cultivation General Discussion [Re: Ora]
    #27468673 - 09/14/21 09:03 PM (2 years, 5 months ago)

Quote:

Ora said:
huh initial testing from a couple people of my GT and APE say they experienced more of a body high and slight dizzyness from lower doses sub 1g. they said their previous experiences they got more of a "head high" and they didnt have this dizzyness/body high. they dont know anything about varieties its just whatever others gave them.

i capped some APE in size 0 caps at 180mg each and they produce way more of an effect than microdose level haha. i dont have smaller caps and these are too strong. or i just need to cut it with something to dilute it i guess.

anyway whats your guys' experience?




i micro at 0.325-0.385 g, lately. i got a tolerance. don't feel it.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,501
Loc: where?
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OG Cultivator
Re: Cultivation General Discussion [Re: SpaceBaby] * 1
    #27468719 - 09/14/21 09:54 PM (2 years, 5 months ago)

ive been microdosing 1g of ape everyday. i feel it man.

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OfflineOra
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Re: Cultivation General Discussion [Re: mushboy]
    #27468724 - 09/14/21 10:04 PM (2 years, 5 months ago)

i think we need an intervention

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InvisibleWall.E
Bacteria's Bitch
Male


Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
Re: Cultivation General Discussion [Re: mushboy]
    #27468731 - 09/14/21 10:15 PM (2 years, 5 months ago)

Quote:

mushboy said:
ive been microdosing 1g of ape everyday. i feel it man.




:spaceman:


--------------------
Life’s shit, but I’m loving it


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OfflineFailboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
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Re: Cultivation General Discussion [Re: mushboy]
    #27468744 - 09/14/21 10:51 PM (2 years, 5 months ago)

Quote:

mushboy said:
ive been microdosing 1g of ape everyday. i feel it man.



Member that last time I drank that Tamp tea and got excited!? I was like "ALL the Bioassays:rockon:!"... that was my most recent dose.:smoker:

I got 16 bags lined up to spawn tomorrow. Got the liners cut, just need to do the rest. Got like 3 dozen bags ready to noc and some damn sexy growth on some MYA :cool:

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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,501
Loc: where?
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OG Cultivator
Re: Cultivation General Discussion [Re: Failboat] * 1
    #27468748 - 09/14/21 11:01 PM (2 years, 5 months ago)

thats always a good answwer. how much did you dose man?

all of it.
:nonoyes:

i got 4 big lanky apes in the fridge i need to clone them but im afraid of them.

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