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Offlinejpchatx
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Best time to do agar transfer?
    #27467070 - 09/13/21 04:51 PM (2 years, 4 months ago)

I’m on my first agar grow, second transfer. I have one plate one plate growing faster and more rhizomous than the others, and was curious if there’s an optimal time to do the next transfer. Eg do I need to wait til it reaches the edges, or does it matter? This is 5 days after transfer, and it’s growing at about 2x the rate of my others (golden teacher from a syringe). Given how healthy it is, I’m wondering if I abandon the other plates and just do another transfer with this one (o started with 3, which became 5 for the first transfer).

Also any segments anyone would recommend in particular?



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OfflineArthurFungarelli
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Re: Best time to do agar transfer? [Re: jpchatx]
    #27467116 - 09/13/21 05:25 PM (2 years, 4 months ago)

I don't do much isolation myself, but if it were my plate (and I were trying to isolate as it sounds like you're trying to do), I'd let it grow another couple of days, transfer a couple wedges from the 9 o'clock area, then chop up the rest and drop it into grain jars.


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Offlinejpchatx
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Re: Best time to do agar transfer? [Re: ArthurFungarelli]
    #27467162 - 09/13/21 06:06 PM (2 years, 4 months ago)

Oh that’s super interesting, I hadn’t considered just going straight to jars. I was planning on a bulk tub grow so was thinking of isolating just so that fruiting is consistent. Is there a chance that going straight to jars at this point will give me a bunch of different growth rates vs waiting for an isolate?

Also any opinion on doing a grain master then g2g transfer vs just going straight to several? I have 10 qt jars with oats ready after a pc.


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OfflineArthurFungarelli
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Re: Best time to do agar transfer? [Re: jpchatx]
    #27467181 - 09/13/21 06:17 PM (2 years, 4 months ago)

There is a chance going straight to jars could give you a bunch of uneven growth, but it's also possible for an untested isolate not to fruit at all. 

If you're after a good canopy, I'd put wedges from this into 10 jars after taking a couple transfers (you've already done some work on the way to isolating it, and you don't have to give that up).  Spawn those jars to some coir, and get yourself some clones started.  Take your clone and run some UnBODified tubs, and you'll have more mushrooms than you know what to do with.

I'd go straight to the 10 jars - drop a large-ish piece of agar (~5mmx20mm) into each jar and shake them just enough that the oats cover the agar.


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Invisiblefungusul
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Re: Best time to do agar transfer? [Re: ArthurFungarelli]
    #27467264 - 09/13/21 07:22 PM (2 years, 4 months ago)

In my opinion, the best time to do a transfer is as soon you see some mycelium growth, maybe the size of a dime. The more you let it grow, the more there is chance for contaminants to catch up and cover the healthy mycelium. Margins tend to be contaminated from water pooling, so it's not good to wait until it gets there.

I would take a transfer from 8-9.


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OfflineOra
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Re: Best time to do agar transfer? [Re: fungusul]
    #27467300 - 09/13/21 07:51 PM (2 years, 4 months ago)

i wouldnt put that to 10 jars. i think a couple more transfers would be better first.


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InvisibleSmartattack
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Re: Best time to do agar transfer? [Re: Ora] * 1
    #27467457 - 09/13/21 10:16 PM (2 years, 4 months ago)

Some of us prefer getting to grain with a new culture sooner rather than later. Once the plates are clean, getting it to grain will give the most genetic variety. This is good for thinking ahead as you may end up with more fruit variety from which to choose a clone to focus on. It's really no more than aesthetically pleasing to carry cultures out many transfers looking for museum grade looking rhizomorphic plates. That's good for Instagram but not the best practice for hunting a winning phenotype from which to clone and ideally slant for ongoing use.

If you are going to take many transfers, many, myself included, like to sample an area where the mycelium has reached furthest from center of the transfer. This way you are at least selecting for something functional like growth speed rather than just what is pretty or "ropey", which unfortunately means nothing for how it will perform.


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Offlinejpchatx
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Re: Best time to do agar transfer? [Re: Smartattack]
    #27467537 - 09/14/21 12:35 AM (2 years, 4 months ago)

Thanks for all these tips. I like the sound of getting to grain faster then sampling a clone from good fruit. I don’t have any need to get a true isolate if it’s not necessarily any better. I’d like to get to my unBODified tub ASAP.

Is there any benefit to saving this plate after transferring to jars, given the real thing I’m after is to find the best fruiter? My wife was more fine with my hobby before she knew I’d need to steal some fridge space :tongue:


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Offlinejpchatx
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Re: Best time to do agar transfer? [Re: jpchatx]
    #27467570 - 09/14/21 02:30 AM (2 years, 4 months ago)

Argh, I just realized something. I have all my supplies, EXCEPT for SFDs. Not sure how I missed this (I used micropore tape with my last grow, but want to do it the "proper" way this time).

It's looking like they won't arrive til next week, at which point I expect this plate to have grown to the edges. Can I just stick it in the fridge to slow it down until my jars are sterilized? Anything I need to watch out for?


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InvisibleGuerrilla
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Registered: 01/30/21
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Re: Best time to do agar transfer? [Re: jpchatx]
    #27467578 - 09/14/21 02:49 AM (2 years, 4 months ago)

Quote:

jpchatx said:
Oh that’s super interesting, I hadn’t considered just going straight to jars. I was planning on a bulk tub grow so was thinking of isolating just so that fruiting is consistent. Is there a chance that going straight to jars at this point will give me a bunch of different growth rates vs waiting for an isolate?

Also any opinion on doing a grain master then g2g transfer vs just going straight to several? I have 10 qt jars with oats ready after a pc.





If you want consistent growth in your tubs then search Josex Poke. You take a microscopic sample, so the genetics are narrowed down much further, whereas with either sending this plate to grain or even isolating further can have mixed results, there's a whole bunch of genetics here.


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Being pissed on does not make you a real man.


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