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Offlinebagga
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Registered: 05/19/21
Posts: 334
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Re: Cultivation General Discussion [Re: Munchauzen]
    #27466785 - 09/13/21 12:39 PM (2 years, 6 months ago)

Haha thanks munchauzen :smile:

I read your Tek, but it sounded like you use it straight away / don't store it or grow it out?

I was planning to use it with speedy culture and fully colonized plates.

Does your approach work with wedges or 1/4 plates?

...Also, I went to all the fuckery of making lids with holes in them just for LC :p

Edited by bagga (09/13/21 12:40 PM)

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OfflineOra
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Re: Cultivation General Discussion [Re: bagga]
    #27466794 - 09/13/21 12:53 PM (2 years, 6 months ago)

thats what i read in the josex poke thread.

i made these like 10 days ago for fun just to try it. I've stopped shaking it like 4 days ago. It's filtered brf lc with agar.



Made them with the intention of one and done. If I opened them I'd just use em all at once.

Edited by Ora (09/13/21 12:57 PM)

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OfflineFailboat
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Re: Cultivation General Discussion [Re: Munchauzen]
    #27466796 - 09/13/21 12:53 PM (2 years, 6 months ago)

Quote:

Munchauzen said:
you can make LC without a GE filter? does somebody have a write-up for that?



I just use media bottles and I tighten em down  to shake. Maybe that's why my LC are slow to colonize, but I also don't swirl em enough. Took a day off to help someone move yesterday and now I'm pretty beat.

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OfflineEnjoil
I'm a teapot


Registered: 10/29/20
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Re: Cultivation General Discussion [Re: Failboat]
    #27466805 - 09/13/21 01:01 PM (2 years, 6 months ago)

These little baby bottles are sweet to use! Just a blob of silicone in the nipple and it’s done.

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OfflineEnjoil
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Registered: 10/29/20
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Re: Cultivation General Discussion [Re: bagga]
    #27466880 - 09/13/21 01:56 PM (2 years, 6 months ago)

Oh, I meant if you splash the filter it might start growing mycelium on it and could be an issue, if your not going to be using it soon…. My bad I should of explained better.

I’m stoned  stupid half the time. And the rest of the time I’m sleeping.

Ha ha. I had to edit cuz my it said I’m stupid stupid! Lol

Edited by Enjoil (09/13/21 03:13 PM)

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Offlinebagga
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Re: Cultivation General Discussion [Re: Enjoil]
    #27466926 - 09/13/21 02:40 PM (2 years, 6 months ago)

Thanks for the ideas and feedback everyone.

I'm gonna abandon the modded lids and give the baby bottle idea a go. I'll report back progress :wink:

Wide mouth, heat safe, easy to pour, cheaper than a media bottle. Awesome idea 😎

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OfflineFailboat
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Re: Cultivation General Discussion [Re: bagga]
    #27466949 - 09/13/21 03:05 PM (2 years, 6 months ago)

You get what you pay for:smoker:

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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Failboat] * 1
    #27466959 - 09/13/21 03:11 PM (2 years, 6 months ago)

I love goooooooooooooooooold



Half turn unscrewed and fixed with parafilm to keep neck clean for free pour & ge.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineEnjoil
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Registered: 10/29/20
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Re: Cultivation General Discussion [Re: Failboat]
    #27466962 - 09/13/21 03:14 PM (2 years, 6 months ago)

Yeah these are like 13 bucks a pop.

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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Enjoil] * 1
    #27466980 - 09/13/21 03:35 PM (2 years, 6 months ago)

Worth every damn penny.

Of course you don't have to spend da coin, these are upcycled mrs braggs bottles with just a foil lid



--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineOra
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Re: Cultivation General Discussion [Re: sandman420]
    #27467113 - 09/13/21 05:24 PM (2 years, 6 months ago)

interesting, the heinz white vinegar glass bottles are the same ones and its like a buck a piece.

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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Ora]
    #27467129 - 09/13/21 05:40 PM (2 years, 6 months ago)

The foil lids work but you have to take them off once to inoculate with agar and they are fragile. Easy to mess it up accidentally. I was using o-rings I had that just happened to fit snug on the neck to kinda seal them but you could use rubber bands after PCing and transferring or probably nothing.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineOra
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Re: Cultivation General Discussion [Re: sandman420]
    #27467131 - 09/13/21 05:41 PM (2 years, 6 months ago)

id assume the original lids would be good too.

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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Ora]
    #27467133 - 09/13/21 05:42 PM (2 years, 6 months ago)

I wouldn't assume that, pc some in a foil pack to test it.

The problem is the inside liner of the lid that actually is the seal melts even if the lid itself is OK.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineOra
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Re: Cultivation General Discussion [Re: sandman420]
    #27467139 - 09/13/21 05:48 PM (2 years, 6 months ago)

usually they use this blue colored silicon liner. isnt silicon really high temp resistant? my sparkling water lids are like that too and when i shake it nothing leaks. if plastic mason jar lids work i dont see why this wont.

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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Ora]
    #27467144 - 09/13/21 05:52 PM (2 years, 6 months ago)

I just tested the lid with a soldering iron that has digital temp control and it totally melts like butter at 250f.

That liner on water bottles is definitely not high temp I promise.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineOra
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Re: Cultivation General Discussion [Re: sandman420]
    #27467146 - 09/13/21 05:53 PM (2 years, 6 months ago)

dang that sucks

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Offlinejomanda1990
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Re: Cultivation General Discussion [Re: Enjoil]
    #27467192 - 09/13/21 06:21 PM (2 years, 6 months ago)

Quote:

Enjoil said:
What? You do know that mushrooms spores can survive traveling through outer space right? I’m sure they will survive in your fridge for a while with or without plastic…




Yes, I do know that, but it's irrelevant to my question. I want to know how to plan for long-term storage of varieties that I don't plan on growing every year. If parameters like refrigeration and air-tight containment help increase the half-life of spores from, say, 2 years to 4 years, then you bet I will keep them like that.


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Offlinebagga
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Registered: 05/19/21
Posts: 334
Last seen: 10 months, 16 hours
Re: Cultivation General Discussion [Re: sandman420]
    #27467221 - 09/13/21 06:41 PM (2 years, 6 months ago)

Wife just hosed the baby bottle idea. Sensitive topic :/

Ended up finding these ($5/ bottle)
https://www.amazon.com/dp/B08XYBDFRL?smid=A29JIFH4ZXPNL8&ref_=chk_typ_imgToDp&th=1


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Offlinebagga
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Re: Cultivation General Discussion [Re: jomanda1990]
    #27467243 - 09/13/21 07:09 PM (2 years, 6 months ago)

When I looked into this, it seems cool stable temp, dry, no air flow, and dark.

Your home fridge is probably bad as temp fluctuation likely worse than a cool dark cupboard in a sealed container.

If you want reliably longer than a few years, there's a research paper that said longest preservation of cultures was achieved by refrigerating myc in distilled water (it goes dormant if prepared properly). I think 14yrs in the paper was the longest.. I could be wrong about that number, but it was a long time.


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_______________________
For personal religious use only. Embrace infinity for a healthier mind.

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