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OfflineMelgo
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Registered: 02/11/16
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Re: Cultivation General Discussion [Re: A.k.a]
    #27466386 - 09/13/21 02:53 AM (2 years, 6 months ago)

Quote:

A.k.a said:
Those look done.


A lot of times the top layer doesn’t really colonize if there’s enough fae to dry them out.




A.k.a, thanks a bunch for the knowledge dropped here! I was wondering why some of my sclerotia jars looked like the top grains never wanted to colonize.

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InvisibleMtbromo
The Bro


Registered: 08/27/20
Posts: 186
Re: Cultivation General Discussion [Re: Melgo]
    #27466402 - 09/13/21 03:51 AM (2 years, 6 months ago)

How long until a spore Prnt is useless for agar work?


How long will a fully col jar of spawn be useable to make more jars?


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Cultivation General Discussion [Re: Mtbromo] * 1
    #27466404 - 09/13/21 03:55 AM (2 years, 6 months ago)

Prints are good for years. Probably 10 years is around the max.

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OfflineSpaceBaby
alchemist
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Registered: 11/01/20
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Re: Cultivation General Discussion [Re: A.k.a]
    #27466425 - 09/13/21 04:47 AM (2 years, 6 months ago)

Quote:

A.k.a said:
Those look done.


A lot of times the top layer doesn’t really colonize if there’s enough fae to dry them out.




Based on this, I plan to put this Enigma to a p9-style shoe later this morning:



:fuckyeahdance:


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: SpaceBaby] * 1
    #27466449 - 09/13/21 05:24 AM (2 years, 6 months ago)

Well my first masters mix bags seemed to come out ok. No bursting or melting bags so I’m happy.



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Offlinepeppel36
I'm a teapot

Registered: 03/02/21
Posts: 67
Loc: Svezia
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: Smoothcat]
    #27466582 - 09/13/21 08:33 AM (2 years, 6 months ago)

Anyone know why I got mini mushies? Look at the little guy on the left.
So far only one fully matured, so maybe the rest will grow bigger, but its about 1½ cm lmao.

Moisture has been fine throughout.


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: peppel36]
    #27466630 - 09/13/21 09:41 AM (2 years, 6 months ago)

just genetics by the look of it. is that MS? also, I will never vouch for unmodded tubs. there could probably be some more FAE going on in there.

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Offlinepeppel36
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Registered: 03/02/21
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Re: Cultivation General Discussion [Re: Munchauzen]
    #27466634 - 09/13/21 09:46 AM (2 years, 6 months ago)

Spawning more tomorrow. I guess I'll try a modded one this time.
Yes it is MSS to grain. And you before you judge me too hard, I have a2g jars coming up. I was just impatient and did this with a syringe I wasn't using while I was cleaning up my agar.

I have 2 more quarts of MSS that I will be spawning today or tomorrow. Will be interesting to see if they turn tiny as well.

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OfflineEnjoil
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Registered: 10/29/20
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Re: Cultivation General Discussion [Re: peppel36]
    #27466639 - 09/13/21 09:51 AM (2 years, 6 months ago)

just Wait til you see a big sucker and clone it.  Maybe the second flush will be bigger?

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Offlinepeppel36
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Re: Cultivation General Discussion [Re: Enjoil]
    #27466640 - 09/13/21 09:52 AM (2 years, 6 months ago)

Yeah I'm going to ride it out and see what happens. It's bittersweet. My first grow and its like this. But at the same time it was pretty funny seeing that tiny little thing in there today. I cut it off and ate it.

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OfflineEnjoil
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Re: Cultivation General Discussion [Re: peppel36]
    #27466647 - 09/13/21 09:58 AM (2 years, 6 months ago)

Dude you should be real happy! I grew mold and every color of the rainbow contamination before I got a mushroom! And it took a whole summer!  I built a damn room out of wood and plastic, quit smoking inside and eventually got it going.

Your off to a great start!

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Offlinepeppel36
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Registered: 03/02/21
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Re: Cultivation General Discussion [Re: Enjoil]
    #27466665 - 09/13/21 10:26 AM (2 years, 6 months ago)

Thanks! It was fun doing it, so I'm definitely going to keep trying.
I've been real confused about contams, because I have had almost none even though my sterile technique is pretty bad. I have had a single agar plate contam with bacteria, out of about 60. No jars so far that I could tell.

I've even gotten pissed off during transfers from failing and started stabbing the agar aggressively to get the damn transfer off. I have dropped plates upside down. I exhaled directly into an open agar plate from close range (5cm or so). No contams.

Makes me wonder how much environment actually plays a role, cause I live in a relatively new, super clean apartment with an air cleaner running 12 hrs a day.

Edited by peppel36 (09/13/21 10:28 AM)

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Offlinebagga
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Re: Cultivation General Discussion [Re: Enjoil]
    #27466671 - 09/13/21 10:33 AM (2 years, 6 months ago)

When mixing LC, how important is it not to splash the filter?

I have a single piece of tivek siliconed to the lid on a pint jar. I used bod's broken glass mixer idea.

The jar is only 1/2 full, but I can't seem to swirl it fast enough to get the glass moving without liquid running up the sides towards the filter.

Have a just over filled it? Or am I being too precious about wetting the filter?


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OfflineEnjoil
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Registered: 10/29/20
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Re: Cultivation General Discussion [Re: bagga]
    #27466686 - 09/13/21 11:03 AM (2 years, 6 months ago)

Well how long do you plan to keep it til you use it? I make a lc and then after about a week  fill up a couple syringes and throw those in the fridge and use the rest on a project.

Don’t be gross, practice good sterile techniques and keep stuff clean. I don’t want to see you back asking “ is this contamination!”? I want to see you posting big beautiful canopies!

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Offlinejomanda1990
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Re: Cultivation General Discussion [Re: Munchauzen]
    #27466687 - 09/13/21 11:05 AM (2 years, 6 months ago)

Quote:

Munchauzen said:
Prints are good for years. Probably 10 years is around the max.




Do you know if refrigeration in a sealed container helps for sporeprints and swabs?


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OfflineEnjoil
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Re: Cultivation General Discussion [Re: jomanda1990]
    #27466698 - 09/13/21 11:15 AM (2 years, 6 months ago)

What? You do know that mushrooms spores can survive traveling through outer space right? I’m sure they will survive in your fridge for a while with or without plastic…

Edited by Enjoil (09/13/21 11:16 AM)

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Offlinebagga
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Re: Cultivation General Discussion [Re: Enjoil]
    #27466735 - 09/13/21 11:58 AM (2 years, 6 months ago)

I appreciate your feedback, but it didn't really answer my question.

Sterile technique is not a problem, I'm fussy about being clean. I normally use gtg, but I'm using slower cultures and also moving into gourmets.

Gtg is easy to keep clean, but I'd like to better understand LC.

I will use it grow out cultures, and also for limited time cold storage so I can cycle between cultures without starting over. I don't need/want slants right now.

Is it critical to keep the tivek filter from being splashed? Is tivek water proof/splash resistant?

I can't work out how to stir it properly without getting it wet.


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Edited by bagga (09/13/21 11:59 AM)

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OfflineOra
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Re: Cultivation General Discussion [Re: bagga]
    #27466758 - 09/13/21 12:18 PM (2 years, 6 months ago)

you can just use completely sealed containers next time since it doesnt need extra air. or you could wait until right before your going to use it to shake it real hard.

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Offlinebagga
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Re: Cultivation General Discussion [Re: Ora]
    #27466771 - 09/13/21 12:29 PM (2 years, 6 months ago)

... Wait... unmod lids are ok for LC?!

Why do nearly all the teks use breathers?

I would much prefer unmod, but I assumed contam risk or some other good reason for the extra fuckery.

:blush:


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Cultivation General Discussion [Re: Ora]
    #27466772 - 09/13/21 12:29 PM (2 years, 6 months ago)

you can make LC without a GE filter? does somebody have a write-up for that?

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