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Offlinebepositivesmile
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Registered: 09/13/21
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Last seen: 1 month, 7 days
LC and BOD's unmodified monotub TEK
    #27466341 - 09/13/21 03:30 AM (1 month, 8 days ago)

Hello dear colleagues !

I am sorry for newbie questions but the more i read forum, the less i understand what i have to do. Please have some minutes of your precious time to explain me some things.

I have 20 pieces of 25ml liquid cultures which are made from B+ spore vials. In the end of process i would like to use BODs unmodified monotub TEK, but i want to be sure i make the start of process correct!

Is it fine i do BODs easy AF oat TEK and inoculate it with my liquid culture and then mix with substrate to do umnodified monotub TEK ? How much of a culture should be injected if using mason jars ? I have hypodermic syringes for that. How much milliliters i should inject in what amount of grain or what size of jars ?

Thank you in advance.


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OfflineBiskop
Fokken Prawns
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Registered: 12/09/17
Posts: 176
Loc: Vietnam 🇻🇳
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Re: LC and BOD's unmodified monotub TEK [Re: bepositivesmile]
    #27466371 - 09/13/21 04:17 AM (1 month, 8 days ago)

Hi and welcome to the Shroomery!

Quote:

bepositivesmile said:
I have 20 pieces of 25ml liquid cultures which are made from B+ spore vials. In the end of process i would like to use BODs unmodified monotub TEK, but i want to be sure i make the start of process correct!





By 20 pieces I’m assuming you mean 20 syringes. Did you make the LC yourself?
If you start from spores you better put it through agar first. If you just dropped the spores into a LC solution it’s probably contaminated and won’t do much good. If you can’t do agar at the moment check out The PF TEK.

I’m a newbie myself, I’m just repeating a few things that I learned on here. Hope it helps!


Edited by Biskop (09/13/21 07:22 AM)


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