Home | Community | Message Board

Cannabis Seeds UK
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
OfflineCarmeloLabadie
Stranger
Registered: 09/13/21
Posts: 1
Last seen: 1 month, 13 days
No pour agar sterilization methods
    #27466296 - 09/13/21 02:40 AM (1 month, 13 days ago)

I'm wanting to get into agar work but the first two attempts didn't go very well. I watched loads of videos and did the sterilize and pour method first. Even though I cleaned the SAB, gloves and sleeves I still got about 70% contaminated. So I tried the no pour method next. After taking everything out of the PC I had loads of excess water in everything even after carefully wrapping with foil. I also had what appeared to be algae in the agar but attribute that to letting the agar boil over on the stir plate, that I can fix.



I'm using a mixture of 60 and 90 mm glass petri dishes for doing culture samples and condiment cups for making agar to grain transfers. I have some jars I can put the 60 mm dishes in but the 90's are too big for them or most other jars for that matter. On a side note the 90's stack better than the 60's and that makes a difference when pouring the agar. I'm pushing it at 5 tall with the 60's before they want to topple. I've looked into the Ziploc twist n lock containers but the 60's want to tip over and the 90's are to big.



What methods do you use to keep moisture infiltration to a minimum during PC'ing?


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineCarmodycaramel
Caramelized
Male User Gallery


Folding@home Statistics
Registered: 04/03/17
Posts: 1,183
Loc: United States Flag
Last seen: 2 hours, 41 minutes
Re: No pour agar sterilization methods [Re: CarmeloLabadie]
    #27466312 - 09/13/21 02:55 AM (1 month, 13 days ago)

Hard for me to understand but what do your no pour containers look like exactly? Are you placing pre poured petri dishes inside of jars and pressure cooking?

EDIT : Maybe I'm just a dunce.

My method for no pour involves 4oz mason jars with a single hole poked in the lid covered in micropore tape. I prepare the agar in the microwave, pour into the jars, screw the lids on and then cover the lids in tinfoil and pressure cook.


--------------------
/0---0---0---0---0---0---0---0---0---0\

\0---0---0---0---0---0---0---0---0---0/


Edited by Carmodycaramel (09/13/21 02:57 AM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
OnlineCreonAntigone
Stranger
Registered: 05/31/21
Posts: 512
Last seen: 17 seconds
Re: No pour agar sterilization methods [Re: CarmeloLabadie]
    #27466315 - 09/13/21 02:57 AM (1 month, 13 days ago)

It's not entirely clear - what kind of container are you doing the no-pours in? Are you just PCing the glass Petri dishes? For how long?

If algae germinates on your plates then they aren't sterile. A proper PC cycle will definitely kill the algae spores. Perhaps you aren't sterilizing long enough or perhaps your PC isn't functioning correctly.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinealaskappalachian
peakbagger

Registered: 10/22/19
Posts: 1,369
Loc: The 49th Dimension
Last seen: 5 hours, 33 minutes
Re: No pour agar sterilization methods [Re: CreonAntigone]
    #27466319 - 09/13/21 03:09 AM (1 month, 13 days ago)

When pouring your agar,  let it cool to about 120 before pouring and that will take care of a lot of your problem with moisture.  With prepours,  prep your agar and pour,  then leave those lids off until the dishes cool THEN lid the plates/dishes and PC.  I have almost zero condensation in mine without any additional work.  Also,  sanitizing things with isopropyl is good,  but won't in the least bit compensate for improper technique.  Not an insult at all; just you are new and need a lot of practice.  Go back and read Bohdi's agar teks and some of the prepour teks n the sticky threads.  They cover all of this and more.  Best of luck.  You do a few runs and actually read those teks CAREFULLY and you'll be transferring to your heart's content in no time.  You got this.


--------------------
It is all one vast awakened thing. I call it the golden eternity. It is perfect.
                                -Kerouac


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineDERRAYLD
Constructus
Male

Registered: 05/13/02
Posts: 3,425
Loc: South Africa
Last seen: 4 hours, 42 minutes
Re: No pour agar sterilization methods [Re: alaskappalachian]
    #27466323 - 09/13/21 03:15 AM (1 month, 13 days ago)

No pours have to be cooled slowly to avoid condensation or you have to wrap the pc or similar to allow the agar to cool slowly.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinealaskappalachian
peakbagger

Registered: 10/22/19
Posts: 1,369
Loc: The 49th Dimension
Last seen: 5 hours, 33 minutes
Re: No pour agar sterilization methods [Re: DERRAYLD]
    #27466335 - 09/13/21 03:25 AM (1 month, 13 days ago)

Untrue.  As I just mentioned,  just let the prepours cool before lidding and you're gravy.  I keep about 150 at a time in rotation and pull those right out after PCing.  Every time.


--------------------
It is all one vast awakened thing. I call it the golden eternity. It is perfect.
                                -Kerouac


Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Temp for pouring agar? oscill8 2,188 1 09/19/01 06:34 PM
by oscill8
* Microwave sterilization method, have to use plastic? SixCee 1,365 6 01/14/03 03:18 AM
by SixCee
* agar
( 1 2 all )
mikearch 2,061 21 10/05/02 03:52 PM
by mikearch
* Pour agar into petri dishes before pc angelko 4,639 5 05/18/03 07:05 PM
by Prisoner#1
* Did my friends sterilization method work? BongHit 779 2 07/21/01 04:35 PM
by Anno
* sterilizing pre poured agar?? Visigoth 1,690 3 04/09/03 03:21 PM
by tripndicular
* Re: Alternate Sterilization Methods? Anonymous 580 3 04/13/00 06:53 PM
by Druggie
* Post deleted by Moe Howard
( 1 2 all )
merk227 3,278 26 07/26/02 12:23 PM
by bluhoney

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta, Tormato
122 topic views. 33 members, 138 guests and 40 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 13 queries.