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Markamello
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Aerial mycelium on agar
#27464987 - 09/12/21 03:32 AM (3 years, 4 months ago) |
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I'm not sure if it's easily visible in these pics but the myc is growing aerial on most of my plates

 
I've transferred 3 times and the myc persists to grow aerial as the plate fully colonises.
Is this simply due to a lack of fea in the petri or is it bacteria piggy backing on the myc?
Edited by Markamello (09/12/21 03:47 AM)
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kanemush
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Re: Aerial mycelium on agar [Re: Markamello]
#27464993 - 09/12/21 04:19 AM (3 years, 4 months ago) |
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nothing wrong with aerial mycelium not everything is rizomorphic. I can't tell from the picture what your saying though so you mean it like has levels and is more 3d? what i do with plates depending what mushroom I have on agar so time varies 3-10 days i remove my cling wrap don't use parafilm just cost so much I just remove the wrap. this seems to make the growth explode where if I leave them fully wrapped there slower to colonize. hardly scientific study this is just what I have noticed so good luck.
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Lostkeys
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Re: Aerial mycelium on agar [Re: kanemush]
#27464995 - 09/12/21 04:28 AM (3 years, 4 months ago) |
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Some cultures just do this, particularly aggressive ones I've found.
-------------------- So heavy I fell through the earth...

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Markamello
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Re: Aerial mycelium on agar [Re: kanemush]
#27465015 - 09/12/21 05:31 AM (3 years, 4 months ago) |
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I yep the growth is 3D. It doesn't stick flat with the agar.
Good to hear that it can be normal but it's got me worried because I'm getting around 20% contam rates atm and I'm trying to reel things in.
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Markamello
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Re: Aerial mycelium on agar [Re: Markamello]
#27467591 - 09/14/21 03:19 AM (3 years, 4 months ago) |
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I've sacrificed a plate to get better pictures of the mycelium growing off the plate and I guess reposted to bump the thread and get further conformation that this plate looks good and it wouldn't be a waste of time putting a plate that looks like this to grain.
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shuna


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Re: Aerial mycelium on agar [Re: Markamello]
#27467707 - 09/14/21 06:59 AM (3 years, 4 months ago) |
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I'm struggling with the same type of 3D mold contamination.
Its fuzzy and disorganized growth, not the straight 2D mycelium growth it should be. Pretty sure its not right and its been driving me nuts too, especially since I'm new to this game.
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InsultingLizard
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Re: Aerial mycelium on agar [Re: shuna]
#27467786 - 09/14/21 08:47 AM (3 years, 4 months ago) |
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Post pictures. It may very well be normal. I've seen both flat and fluffy mushroom mycelium, and flat and fluffy mold mycelium. You have to look at everything: mycelium color, presence of spores, small-scale shape, large-scale shape
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Lostkeys
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Re: Aerial mycelium on agar [Re: Markamello]
#27467820 - 09/14/21 09:22 AM (3 years, 4 months ago) |
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Quote:
Markamello said: I've sacrificed a plate to get better pictures of the mycelium growing off the plate and I guess reposted to bump the thread and get further conformation that this plate looks good and it wouldn't be a waste of time putting a plate that looks like this to grain.

Looks good to me, just floofy, although I would probably take one more transfer to get more even growth.
-------------------- So heavy I fell through the earth...

Edited by Lostkeys (09/14/21 09:23 AM)
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milkboy
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Re: Aerial mycelium on agar [Re: Lostkeys]
#27467829 - 09/14/21 09:31 AM (3 years, 4 months ago) |
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Quote:
Lostkeys said: Some cultures just do this, particularly aggressive ones I've found.
So true. I have an Amazonian that if I posted the pic of the agar plate im sure people would have screamed white mold but threw that baby on grain and its growing beautiful, even, fast rhizo.
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shuna


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Quote:
InsultingLizard said: Post pictures. It may very well be normal. I've seen both flat and fluffy mushroom mycelium, and flat and fluffy mold mycelium. You have to look at everything: mycelium color, presence of spores, small-scale shape, large-scale shape

These are from a spore print, but look nothing like the mycelium I had previously used to get the fruit/spores. 
Got a microscope I can use at work. Molds produce spores, whereas the mycelium will only produce spores after fruiting?
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Edited by shuna (09/14/21 09:52 AM)
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InsultingLizard
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Re: Aerial mycelium on agar [Re: shuna] 1
#27467859 - 09/14/21 10:13 AM (3 years, 4 months ago) |
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That looks like it could be mushroom mycelium. How long since inoculation? If you have a microscope, use it. Mold is easy to distinguish under the microscope.
Mold (with spores):

Mushroom:

Mold has a sort of tree-like shape, while mushroom mycelium is more like a web, with the hyphae laid out in straight lines.
Quote:
Molds produce spores, whereas the mycelium will only produce spores after fruiting?
Well, no. It's the fruits themselves which produce the spores. That's their function.
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shuna


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Quote:
That looks like it could be mushroom mycelium. How long since inoculation?
8 days ago, which seems a little fast. But there's lots of variables I guess, temperature, agar mixture... Thanks for the mold pics.
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InsultingLizard
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Re: Aerial mycelium on agar [Re: shuna] 1
#27467892 - 09/14/21 10:34 AM (3 years, 4 months ago) |
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It's probably not mold, then. Mold would germinate within a couple days. One week is typical germination for cubensis.
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Markamello
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So the way to be absolutely certain there is no bacteria is to check under a microscope.
Do you know what magnification these images are at? Or what is suitable to view mycelium/bacteria?
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InsultingLizard
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Re: Aerial mycelium on agar [Re: Markamello]
#27469542 - 09/15/21 03:51 PM (3 years, 4 months ago) |
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Forget it, you won't be able to see bacteria on agar. On a slide with a cover glass and at 1000X magnification with oil between the lens and the cover they're barely visible.
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shuna


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Re: Aerial mycelium on agar [Re: Markamello]
#27469614 - 09/15/21 05:05 PM (3 years, 4 months ago) |
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Here's some microscope pics of my suspect petris.
x40

x100

x400
  
There is clusters of possibly spores throughout the hyphae. On second thoughts that could be my agar not being perfectly clear... 
Markmello, you could maybe try putting a wedge to a jar of brf+verm mix, heard that will allow the mycelium to outrun the bacteria if present.
If there is mold mixed in with the mycelium then keep making transfers. Not sure what else to do but start over from scratch. A josex poke maybe worth a try.
Good read about hidden contams: https://www.shroomery.org/forums/showflat.php/Number/22020260
-------------------- Moopers nat guide
Edited by shuna (09/15/21 07:37 PM)
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