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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 2 days, 12 hours
Re: Cultivation General Discussion [Re: TedsDead]
    #27461091 - 09/08/21 10:50 PM (2 years, 6 months ago)

Why are there more than one sector?  And what does a monoculture do when u finally achieve one?  Still have never taken a clone.  Very interested.  :takingnotes:


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Offlinef 25 or6to4
Stranger
Registered: 09/04/21
Posts: 3
Last seen: 2 years, 3 months
Re: Cultivation General Discussion [Re: Mad Season]
    #27461094 - 09/08/21 10:53 PM (2 years, 6 months ago)

Noob here. Recently had bruising as a result of using a fan directly.

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Offlinewahhwahh
stupid idiot


Registered: 07/19/21
Posts: 296
Last seen: 2 years, 1 day
Re: Cultivation General Discussion [Re: Crackatoa]
    #27461101 - 09/08/21 11:02 PM (2 years, 6 months ago)

Quote:

Crackatoa said:
Quote:

wahhwahh said:
Oh fuck. That sucks. He's been the most influential poster on what I've been doing :frown:




What you need brother?





I appreciate it!, but I don't really need help, just have been emulating a lot of his tek. It's going really good!

I think I really just need an FFU and I'm golden. SAB hasn't given me much contam, but holy shit it's a pain to use. I fucking dread pouring agar, and I need to, I'm out of plates.

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OfflineParacosm
Female User Gallery

Registered: 07/04/21
Posts: 808
Loc: Floating in Space
Last seen: 5 months, 24 days
Re: Cultivation General Discussion [Re: wahhwahh]
    #27461121 - 09/08/21 11:22 PM (2 years, 6 months ago)

P9 will be back. From what I understand he took a break for a month.


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My Grow Journal

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InvisibleTedsDead
 User Gallery


Registered: 01/03/17
Posts: 5,003
Trusted Cultivator
Re: Cultivation General Discussion [Re: AtmozFear]
    #27461123 - 09/08/21 11:24 PM (2 years, 6 months ago)

Quote:

AtmozFear said:
Why are there more than one sector?  And what does a monoculture do when u finally achieve one?  Still have never taken a clone.  Very interested.  :takingnotes:





more than one sector could indicate different strains.  a monoculture will express the genes of a single strain.  that could be good or it could be not so good.  if you have say a clone plate and you make a transfer to a new plate and see a tiny bit of sectoring but you take it to grain and it kicks ass then I wouldn't worry about isolating it further.  if it sucked tho I wouldnt attribute that to there being different sectors.  in that case I would just go back to ms and look for a new clone candidate to take and test


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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OfflineParacosm
Female User Gallery

Registered: 07/04/21
Posts: 808
Loc: Floating in Space
Last seen: 5 months, 24 days
Re: Cultivation General Discussion [Re: TedsDead]
    #27461124 - 09/08/21 11:27 PM (2 years, 6 months ago)

Thanks, Ted. :takingnotes:


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My Grow Journal

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Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Cultivation General Discussion [Re: Paracosm]
    #27461142 - 09/09/21 12:14 AM (2 years, 6 months ago)

Yeah most clones show some sectoring ime

Not necessarily a bad thing though, most people dont really need a true isolate

Sometimes clones (or even ms) with multiple strains present will be just as organized as an isolate, to a point where its hard to distinguish it from an isolate. 

I have isolated a few cool strains from clone sectors though, but its not really useful for anything but strain hunting


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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InvisibleTedsDead
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Registered: 01/03/17
Posts: 5,003
Trusted Cultivator
Re: Cultivation General Discussion [Re: c10h12n2o]
    #27461168 - 09/09/21 01:23 AM (2 years, 6 months ago)

Exactlty. And If the strains perform well together theres no need to seperate em


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Cultivation General Discussion [Re: TedsDead]
    #27461179 - 09/09/21 01:59 AM (2 years, 6 months ago)

as far as i understand, might even be problematic to separate them

i really wish i knew more about the dynamics of multistrain vs isolated strains. the way strains interact in a colony is pretty fascinating but i dont even know where to begin to learn more about it, besides experimentation

if anyone knows any good resources on this topic, for any mushrooms not just actives, please share


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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InvisibleGuerrilla
Bumbaclart

Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Cultivation General Discussion [Re: c10h12n2o]
    #27461181 - 09/09/21 02:03 AM (2 years, 6 months ago)

Quote:

c10h12n2o said:
as far as i understand, might even be problematic to separate them

i really wish i knew more about the dynamics of multistrain vs isolated strains. the way strains interact in a colony is pretty fascinating but i dont even know where to begin to learn more about it, besides experimentation

if anyone knows any good resources on this topic, for any mushrooms not just actives, please share





I'm just about to experiment with this myself. I have taken 7 different transfers from one clone plate and I'll see how the results differ.


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Being pissed on does not make you a real man.

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Invisiblec10h12n2o
serial dilutor
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Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Cultivation General Discussion [Re: Guerrilla] * 2
    #27461185 - 09/09/21 02:22 AM (2 years, 6 months ago)

you gotta have your variables VERY standardized and run everything with lots of redundancy to even be able to tell, its kinda a pain in the ass

id say at least 3 of each thing you are testing, ideally way more

if you have something that performs well, it really isnt worth the effort unless you just enjoy strain hunting

the VAST majority of deviation in grows is more to do with environmental/prep factors than genetics. this is why even when working with the exact same genetics you will see vastly different results from different growers (in terms of fruit morphology, yields, canopies, etc). even when its the same grower subtle differences can make a huge difference. standardizing things to a point where you can get meaningful data on genetics is pretty difficult

i had a pretty in depth post here for anyone interested in my personal methods for strain hunting: https://www.shroomery.org/forums/showflat.php/Number/27421903#27421903


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 6 months, 14 days
Re: Cultivation General Discussion [Re: BrownBear]
    #27461255 - 09/09/21 05:07 AM (2 years, 6 months ago)

Quote:

BrownBear said:
I think he banned himself.




I think some "noob bullshit" sent him round he twist and he took a break. Noticed the fuckery and not the love. :shrug:

I look forward to his return. His TEKs have been bery bery good to me.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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OfflineBrownBear
Warrior-Traveler
 User Gallery


Registered: 06/05/20
Posts: 1,539
Last seen: 10 months, 4 days
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27461265 - 09/09/21 05:19 AM (2 years, 6 months ago)

Quote:

SpaceBaby said:
Quote:

BrownBear said:
I think he banned himself.




I think some "noob bullshit" sent him round he twist and he took a break. Noticed the fuckery and not the love. :shrug:

I look forward to his return. His TEKs have been bery bery good to me.




P9 is a very knowledgeable member and very helpful. I like when he is around.

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InvisibleGuerrilla
Bumbaclart

Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27461267 - 09/09/21 05:25 AM (2 years, 6 months ago)

Quote:

SpaceBaby said:
Quote:

BrownBear said:
I think he banned himself.




I think some "noob bullshit" sent him round he twist and he took a break. Noticed the fuckery and not the love. :shrug:

I look forward to his return. His TEKs have been bery bery good to me.




Spot on mate. Exactly that lol


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Being pissed on does not make you a real man.

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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: BrownBear]
    #27461317 - 09/09/21 05:48 AM (2 years, 6 months ago)

Quote:

BrownBear said:
Quote:

SpaceBaby said:
Quote:

BrownBear said:
I think he banned himself.




I think some "noob bullshit" sent him round he twist and he took a break. Noticed the fuckery and not the love. :shrug:

I look forward to his return. His TEKs have been bery bery good to me.




P9 is a very knowledgeable member and very helpful. I like when he is around.




Got booty tickled because he thought it was the worst idea in the world to use a sanitized poodle for spawn.
I guess I should test that on petris since primal didn't, yet.


I miss him too.

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OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,898
Loc: Gaming the system
Last seen: 3 hours, 55 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: QM33]
    #27461394 - 09/09/21 07:37 AM (2 years, 6 months ago)

I think he self bans on a regular basis.


Do people think sectoring is bad?? I’ve never gotten that from anything I’ve seen here.


I just think of each good culture like a voltron. If you remove a sector it’s like cutting a leg off.


Now if you’ve got a culture that needs some work removing a sector could be like cutting a huge weight off and now it can really move.


:pipesmoke2:


--------------------
LAGM2020

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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: A.k.a]
    #27461430 - 09/09/21 07:57 AM (2 years, 6 months ago)

Well I hope he's back soon. I'm going to make some petris in actually petris, not Togo cups, so I can test sanitized items on it. Also im going to test some containers, like Togo cups, coke bags, ziplocks. Rather than pouring directly in them tho I'll take transfers from petris and put them in to different containers to test their sterility.

I've also wondered that aka, was going to ask the other day if if isolate is preferable. I wouldn't see any reason so except maybe on terms of vigor, maybe an isolate would grow fast because it all "working together" idk.

But I've had some culture I could keep transferring and they always seem to sector a little...

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OfflineTheBoJim
Strangest
 User Gallery

Registered: 07/23/20
Posts: 449
Last seen: 1 year, 13 days
Re: Cultivation General Discussion [Re: QM33]
    #27461443 - 09/09/21 08:10 AM (2 years, 6 months ago)

P9 is still posting videos on his YouTube if you need a fix :smile:


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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: TheBoJim]
    #27461471 - 09/09/21 08:41 AM (2 years, 6 months ago)

Has anyone adding agar to LC noticed anything about identifying contams?
Like, it makes it hard to see if it's turbid...
Also im wondering if I can expect to see present molds or bacteria growing on the surface if there is a contam?

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OfflineOra
Stranger
 User Gallery

Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 3 months
Re: Cultivation General Discussion [Re: QM33]
    #27461549 - 09/09/21 09:43 AM (2 years, 6 months ago)

Isn't lc impossible to know until you test on agar? (Besides really obvious things like green growth)

Anyone know why APE get all soft? Is it just ripening/rotting? Does it lose potency or something?

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